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Articles by M. Zamri-Saad
Total Records ( 8 ) for M. Zamri-Saad
  A.B.Z. Zuki , A. Fadilah , M. Zamri-Saad , M.Y. Loqman , Y. Norimah and H. Asnah
  The present study was designed to evaluate and compare the natural coral and Calcium Phosphate Cement (CPC) post-implantation in sheep femoral bone. Twenty one adult, male sheep (weight 15-20 kg) were used in this study and were divided into two groups. Group one consists of 12 animals and implanted with natural coral while group two consists of 9 animals and implanted with CPC material. The large cortical defect (2.5x0.5x0.5 cm) was created surgically on the left proximal femur and replaced by the implant. Radiographs and ultrasonographs were obtained immediately after surgery and at 2, 4, 8 and 12 weeks post-implantation. Both ultrasonographs and radiographs taken at 8 and 12 weeks showed that the implants had been resorbed and left the space that much reduced in size. There was no sign of implant rejection observed in all animals. The sheep were euthanased at 2, 4, 8 and 12 weeks post-implantation and the bone examined grossly. Samples of the implant were taken for histological examination. Microscopically, natural coral exhibited rapid resorption and progressively replaced by new bone. At 8 weeks post-implantation, there was no more coral implant present and by week 12 the implant site was almost completely closed and filled by mature bone. Meanwhile, CPC implant was clearly seen and demonstrated only marginal bone formation at the end of 12 weeks study. The coral implant exhibited good bone substitute, but it has fast resorption rate. Thus, it may suitable for less compact bone with small defect.
  M.Y. Sabri , M. Zamri-Saad , R. Son and Sharifah
  Whole-cells DNA of Mannheimia haemolytica A2, A7, A9 and Pasteurella trehalosi, isolated from goats were subjected to plasmid screening and random amplified polymorphic DNA (RAPD) analysis. The plasmid DNA screening revealed the presence of plasmid only in P. trehalosi isolated from goat. The plasmid was 4825 bp. The RAPD analysis indicated similarities and differences among isolates M. haemolytica and P. trehalosi. However, OPA16 primer revealed the DNA fingerprinting pattern that was able to clearly distinguish the various isolated while OPA14 primer revealed less distinguishable pattern. OPA20 revealed a fairly distinguishable DNA pattern.
  A. Ernie , M. Zamri-Saad , Z. Zunita , A. R. Omar , B. Siti Khairani and M. Y. Sabri
  The fimbrial gene of Pasteurella multocida B:2, isolated from cattle with haemorrhagic septicaemia was amplified, cloned, sequenced and expressed in a vector. Cloning of the fimbrial gene revealed a 435 bp band while sequencing revealed similar nucleotide sequencing to the PtfA gene of P. multocida A:3 isolated from chicken, except the five nucleotide changes at residues 171, 282, 363, 387 and 414. The fimbrial gene was found to encode a deduced protein of 144 amino acids. The expressed fimbrial protein was detected from 4 h post-induction with a molecular weight of 18-kDa.
  Khairani-Bejo , S., A. R. Bahaman , M. Zamri-Saad and A. R. Mutalib
  The survival time of Leptospira interrogans serovar hardjo in water, soil and cattle urine in Malaysia were evaluated. The survival time of serovar hardjo in the six types of water samples varied considerably. The longest survival time recorded was for 264 hours (11 days) in river water with between pH 6.7 and pH 7.3 placed in shaded area. The shortest survival time was recorded in seawater pH 6.5 to pH 6.8, with dissolved solid salt content between 3.78% and 3.85%. The leptospires were killed almost immediately after inoculation into seawater. The survival time of serovar hardjo in the three types of soil with three different water content, kept under either direct sun or shaded area were variable. The minimum survival time was 2 hours and maximum was 144 hours (6 days). Serovar hardjo survived in undiluted urine for only 2 hours under direct sun and 6 hours under shaded area. The survival time of serovar hardjo was longer in urine diluted with distilled water compared to undiluted urine. In diluted urine under direct sun, serovar hardjo can survive for up to 48 hours (2 days), under shaded area for up to 72 hours (3 days) and in refrigerator (4 C) for 984 hours (41 days). Under experimental conditions serovar hardjo survived in water, soil and urine remained infective up to 1 to 7 days post inoculation. These findings suggested that survived organisms would provide a source of infection for human and animals exposed to the contaminated environment.
  M. Zamri-Saad and M.S. Shafarin
  Haemorrhagic Septicaemia (HS) is an important tropical disease of cattle and buffaloes caused by Pasteurella multocida B:2. It usually leads to outbreaks causing acute deaths. This study was conducted to determine the response shown by goats following different routes of infection by P. multocida B: 2. Twenty healthy local goats were selected and divided into four equal groups before they were injected with dexamethasone at the rate of 1 mg kg 1 for four consecutive days. On day 4, goats of groups 1, 2 and 3 were inoculatedintratracheal, subcutaneous and intranasal, respectively with 1 ml of inoculum containing 109 cfu mL of live P. multocida B: 2. Goats of group 4 were the negative control receiving no treatment. During the 4-week experimental period, two (40%) goats of group 1 were killed for humane reason; one within 24 h and the other at day 4 post-infection. Three (60%) goats of group 2 were killed, each on days 2, 5 and 14post-infection while two (40%) goats of group 3 were killed on days 20 and 32 post-infection. Post-mortem examination revealed that goats that were killed within the first 2 days post-infection showed lesions typical of HS. Goats that were killed between days 3 and 7 showed evidence of acute pneumonia while those that were killed between days 8 and 14 p.i. showed subacute pneumonia affecting 25% of the lungs. P. multocida B: 2 was successfully isolated from the lungs, lymph nodes, spleens, tonsils, heart blood, livers and subcutaneous fluid of goats of groups 1 and 2 that were killed peracutely. Isolation was successful from the heart blood and subcutaneous fluid of goat of group 2 that were killed acutely on day 5. However, isolation was unsuccessful from goats that were killed after day 14 of infection.
  Saw Po Po , A. B. Z. Zuki , M. Zamri-Saad , A. Rahman-Omar and A. W. Effendy
  The distribution and morphological features of bronchus associated lymphoid tissue (BALT) were evaluated in three months old calves. Five types of lymphoid tissue were found but their distribution varied between regions and individuals. Scattered lymphoid cells were most predominant form. Dense lymphoid aggregates were frequently seen in bronchioles. Follicular lymphoid tissues were seen in bronchi. Small numbers of intraepithelial lymphoid cells were always located throughout the epithelium of mucosa. The intraluminal lymphocytes were observed mainly in bronchi and large bronchioles. The follicular development of BALT was less frequent and only occurred in the large bronchi of anterior cranial lobe in calves 3. The lymphoepithelium invagination of bronchi can be found in calve 1 as in that of duodenum of gut. The occurrence and morphology of BALT were different within the individual.
  S. Nor-Satinati , A.B.Z. Zuki , M. Zamri-Saad , A.J. Awang-Hazmi and Saw Po Po
  The present study was conducted with the aim to investigate the response of GALT following oral administration of P. multocida type B2. A total of 12 adults, Spraque-dawley rats, supplied by the animal breeding unit, Faculty of Veterinary Medicine, UPM, were used in this study. The rats were divided into 2 groups, which consist of 6 rats per group. 0.5 mL of 104 CFU of P. multocida B2 was given to the rats in first group through intranasal route by using 1 mL syringe while the second group was given 0.5 mL normal saline using the same method. Both groups were kept in cages separately for 2 weeks before being euthanased. The number and size of Peyer’s patches of the intestine were counted and measured macroscopically upon euthanasation. The intestine was then divided into 14 portions; proximal, middle and distal of each the duodenum, jejunum, ileum and colon and one each for caecum and rectum for histological evaluation. The Hematoxylin and Eosin staining was used to count the number of lymphocytes follicles and intraepithelial lymphocytes. This stain also used to measure the size of lymphoid follicles. The Methyl Green Pyronin was used to count the number of plasma cell. The results showed that intranasal administration of Pasteurella multocida B2 has slight influence on the development of GALT. However, the different in the parameters study between control and infected groups were not significant (p>0.05).
  S.O. Sarah , M. Zamri-Saad , Z. Zunita and A.R. Raha
  Pasteurella multocida has been recognised as an important pathogen of animals that causes a wide spectrum of diseases, collectively termed as pasteurellosis. The bacteria possess several housekeeping genes that maintain the viability of the bacteria. One of the housekeeping genes is the gdhAgene that serves as a major link between carbon and nitrogen metabolism. The full length gdhA gene of P. multocida B:2 was found to be 1180bp while the functional fragment of the gene was 652bp. The gene was successfully sequenced and later cloned into E. coli pCR 2.1-TOPO vector.
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