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Articles by M. Siddique
Total Records ( 2 ) for M. Siddique
  M.A. Ilyas , I. Hussain , M. Siddique and M.S. Mahmood
  An inactivated vaccine was prepared from local isolates of egg drop syndrome (EDS) virus using aluminum hydroxide as an adjuvant. A total of 30 commercial layers at the age of 16 weeks were procured and divided in to two groups, A and B having 20 and 10 birds, respectively. The birds of group A were vaccinated with 0.5ml aluminium hydroxide gel adsorbed EDS vaccine. The birds of group B were kept as unvaccinated control. The serum samples were collected at a 14-day interval till 6 weeks post vaccination. At 22 weeks of age group A was again divided into two groups A1 and A2. Birds of group A2 and group B were challenged with a virulent EDS virus. The results revealed that birds of group A had HI titer ranging from 1:128 to 1:512 at different times of sample collection with a geometric mean titer 116. Highest antibody response appeared on 4th week post vaccination. Results also revealed that the vaccination protected the birds against challenge with the virulent EDS virus. Where there was a significant drop in egg production in unvaccinated challenged birds. The egg quality was also deteriorated as miss-shapened, soft-shelled and shell-less eggs were laid in EDS unvaccinated layers.
  M.S. Mahmood , M. Siddique and I. Hussain
  Isolation of infectious bronchitis virus (IBV) was conducted in asymptomatic layer flocks with a history of IB or IB vaccination at least from 5 to 20 weeks before the day of sampling. A trypsin-induced hemagglutination (THA) assay to detect IBV in allantoic fluid of embryonated eggs was used. Detection of IBV through THA showed that group A [5 wks post-inoculation (PI)] was 100%, group B (10 wks PI) was 86%, group C (15 wks PI) was 74% and group D (20 wks PI) was 28% positive for IBV. Virus titre was found negatively correlated with the PI duration.
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