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Articles by Lily Syukriani
Total Records ( 5 ) for Lily Syukriani
  Jamsari , Lily Syukriani , Hashlin Pascananda Utami , Friedrich Herberg , Wolfgang Nellen and Istino Ferita
  Artificial inoculation is an important method for understanding the plant-virus interaction. Currently available techniques for virus artificial infection are whitefly based inoculation, grafting, mechanical, agroinoculation and particle bombardment. In fact, almost all the available inoculation methods do not result in ideal efficiency nor practicality. The purpose of this study is to establish an injection based technique for routine artificial inoculation of Geminivirus particle into chilli pepper plant. The following treatments were applied in this study: Different ratios of extract-buffer (0, 20 and 60% w/v) and injection at four different positions (no inoculation, shoot, middle of main vein and petiol). The presence of Geminivirus particles in plants whether for inoculum source and infection effectivity was confirmed using the polymerase chain reaction. Some morphological observations were also applied in measurement of infection effectivity. Overall, the incidence of infection was 18% and among these, the average intensity of disease was 53% in the four week post inoculation. Geminivirus transmission by injection technique described in this study could be a simple and effective method for geminivirus artificial infection. However, accuracy, simplicity and uniformity have to be accommodated for further application of this technique.
  Jamsari , Istino Ferita , Lily Syukriani , Helni Lalan , Friedrich Herberg and Wolfgang Nellen
  Whiteflies have attracted intensive attention from many agriculturists due to their direct feeding and plant virus transmission. The economic losses depend on certain species population existing on the field during chilli-pepper cultivation. More over, biotype characteristic will determine the effectivity of applied pest management. Based on this, detailed information on whiteflies in chilli-pepper cultivation is very important. We applied mitochondria cytochrome oxidase unit I gene sequence for detecting species and biotype of whiteflies existing in West Sumatera. Two species were successfully identified i.e., Bemisia tabaci and Trialeourides vaporariorum that are dominant in this region. Distribution of both species are separated by altitude, where B. tabaci exist predominantly from low altitude to medium altitude, whereas in high altitude it is not common. On the other hand, T. vaporariorum distribute mainly in high altitude and not in medium altitude. Furthermore biotype determination on B. tabaci identified the existence of B biotype in all areas studied.
  Siti Nur Aisyah , Sulastri Sulastri , Retmi Retmi , Rahmi Henda Yani , Elly Syafriani , Lily Syukriani , Fatchiyah Fatchiyah , Amri Bakhtiar and Jamsari Jamsari
  Background and Objective: Plant-associated bacteria, such as Phyllobacterium play a significant role in protecting plant from pathogenic fungal infection. These Phyllobacterium are known to be existed in such a microbial community with various microbes thus leading to elevated disease suppression. The aim of this study was to assess the fungal suppression activity of indigenous Phyllobacterium isolates and its strain/species compatibility with rhizobacteria. Materials and Methods: Two indigenous Phyllobacterium isolates were identified using 16S rRNA gene sequence and its antifungal activities were tested against several phytopathogenic fungus. Further antagonistic assay was performed to compare the efficacy of cell culture and cell-free supernatants. Its compatibility was assayed by performing the antifungal assay using the combination of these Phyllobacterium isolates with rhizobacteria ones. Data were statistically analyzed using one-way analysis of variance and the significance was further processed using Duncan’s new multiple range test with a p<0.05. Results: Both isolates (UBCF_01 and UBCF_13), identified as Serratia plymuthica, exhibited higher suppression activity against Colletotrichum gloeosporioides compared to Fusarium oxysporum and Sclerotium rolfsii. Both isolates revealed opposite trend in their activities resulted from cultured cells and cell-free supernatants. Furthermore, the better suppression efficacy of the culture supernatants was resulted from single cultured cells, instead of co-culture. However, both isolates displayed quite poor compatibility with rhizobacteria isolates. Conclusion: These indigenous Phyllobacterium showed promising ability to be used as biocontrol agents for anthracnose. The application of its culture supernatants offered the less hazardous option of biological control implementation. However, their poor compatibility, even with the same species (rhizospheric UBCR_12) might be occurred due to habitat differences.
  Jamsari Jamsari , Istino Ferita , Ade Noverta , Eko Dharma Husada , Friedrich W. Herberg , Wolfgang Nellen and Lily Syukriani
  Background and Objective: In order to elucidate pathogenic determining factor of pepper yellow leaf curl virus isolated from a lowland chili pepper cultivation field in West Sumatra, Indonesia, a whole genome analysis via primer walking strategy was conducted. Materials and Methods: The whole DNA A-like genome sequence of PepYLCIpsV was elucidated via five steps of primer walking, started with universal primers PAR1c715 and PAL1v1978 as the start point. Results: Whole genome comparison analysis identified only one single base insertion/deletion event located in the common region from a previously described isolate collected from the upland location (PepYLCItdV). The BLAST comparison on the nucleotide level showed 90.6% maximal homology with the existing DNA A-like genome from many pepper yellow leaf curl viruses deposited in public database so far. Further detail comparison each of six Open Reading Frames (ORFs) between PepYLCIpsV and PpepYLCItdV indicated that V1 and V2 displayed 94-95% homology, respectively, while C2 and C3 had homology in range of 99 and 97%, respectively. Interestingly C1 and C4 showed homology only 79 and 68%, respectively and the Common Region (CR) shared only 74% similarity. Conclusion: The C1 and CR could be the determining factors for the pathogenicity, therefore, characterizing of these two regions in the population could be used for management and controlling of the virus. The pathogenic isolate PepYLCIpsV (KT809345) appears to be derived by recombination from two isolates originating from different regions and hosts. Its existence seems to be more ancient than its currently mild dominant counterpart PepYLCItdV (KT809346).
  Siti Nur Aisyah , Jefri Maldoni , Irma Sulastri , Weni Suryati , Yuli Marlisa , Lissa Herliana , Lily Syukriani , Renfiyeni Renfiyeni and Jamsari Jamsari
  Background and Objective: Assessment of the plant growth promoting (PGP) properties of a bacteria in vitro required the adjustment of appropriate culture condition. This study was aimed to optimize the culture condition required to stimulate two PGP traits (antifungal activity and indole-3-acetic acid (IAA) production) of phylloplane Serratia plymuthica strain UBCF_13. Materials and Methods: Evaluation of UBCF_13 antifungal activity against F. oxysporum f. sp. glycine was conducted under various modifications (pH and addition of exogenous carbons, Nitrogens as well as metals). This strain IAA production was optimized under various culture durations and L-tryptophan (Trp) concentrations. The resulted IAA were then inoculated to several plant’s seed to evaluate its effect on plant root and shoot growth. Data were analyzed statistically and the significance was further assessed using Duncan’s new multiple range test with a p<0.05. Results: Application of 50 μL cells-free supernatants (CFS) exhibited the highest fungal suppression under pH 5 (23.02%), (NH4)2 SO4 addition (28.25%) and CaCl2 addition (27.14%). The highest IAA concentration (116.09 μg mL1) was obtained from the 48 h culture containing 0.2% L-Trp. The IAA-containing CFS of UBCF_13 stimulated longer growth in root and shoot of Solanaceous plants (chili, tomato and eggplant). Conclusion: Antifungal activity of UBCF_13 was predicted to be dependent on acidic condition and the availability of inorganic nitrogen as well as calcium, while its IAA could be produced in short time with the low amount of Trp.
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