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Articles by Li Pan
Total Records ( 6 ) for Li Pan
  Hua Tang , Xin-Sheng Liu , Yu-Zhen Fang , Li Pan , Zhong-Wang Zhang , Peng Zhou , Jian-Liang Lv , Shou-Tian Jiang , Wen-Fa Hu , Pan Zhang , Yong-Lu Wang and Yong-Guang Zhang
  The Foot-and-mouth Disease (FMD), which is the most devastating disease of livestock husbandry, drastically hinders the development of stock raising. At present the main FMD vaccine is still traditional inactivated vaccine, as its good performance in protective activity and immune efficacy, it is widely used in the world for preventing and controlling this disease, especially in developing countries. However, people have realized many disadvantages of inactivated vaccine. In order to resolve these problems, the scientists are conducting some new research about novel FMD vaccine to seek for more secure and effective vaccines. Nowadays, with the rapid development of molecular biology, researchers are paying high emphasis on novel FMD vaccines, such as, synthetic peptide vaccine, epitope-based vaccine, DNA vaccine, chimeric vaccine and so on. There is no doubt that these new-type FMD vaccines will make great contribution to the prevention and control of FMD. This study reviewed the international research progress on the traditional vaccines and novel vaccines of FMD.
  Hua Tang , Xin Sheng Liu , Yu Zhen Fang , Li Pan , Zhong Wang Zhang , Peng Zhou , Jian Liang Lv , Shou Tian Jiang , Wen Fa Hu , Pan Zhang , Yong Lu Wang and Yong Guang Zhang
  In recent years, as the genome sequencing technology and protein peptide chemical synthesis technology become mature, especially the proposal of epitopic biology, more and more Foot-and- mouth Disease Virus (FMDV) antigenic epitopes are revealed. Studies on epitopes have greatly promoted the development of new-type vaccines since the early 1980s. Thanks to the consecutive discovery of new epitopes, people can overcome the Major Histocompatibility Complex (MHC) restriction between species more easily. Researchers could also produce poly-vaccine containing epitopes of different serotypes. The selected epitope sequences are highly conserved in different serotypes and this is the key guarantee of cross immune response of vaccine. It is obvious that epitope will play a key role in elaborating new-type FMDV vaccines. This study summarized the epitopes of FMDV.
  Hua Tang , Xin-Sheng Liu , Yu-Zhen Fang , Li Pan , Zhong-Wang Zhang , Peng Zhou , Jian-Liang Lv , Shou-Tian Jiang , Wen-Fa Hu , Pan Zhang , Yong-Guang Zhang and Yong-Lu Wang
  The Z-curve is a geometrical tool for visualizing and comparing genomes. Since, the curve contains the information carried by the given sequence, DNA sequences could be analyzed systematically. In this study, the ORF5 gene of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) was analyzed by the Z-curve method and the DNAstar (DNASTAR Inc.) computer programs. The results revealed by these two methods were identical. The phylogenetic tree is more digitized and the Z-curve method is more picturesque. It turned out to be that, the Z-curve method shows a widely application prospect in phylogenetic relationships analysis. However, the Z-curve method is still in its premature stage. Novel algorithms are expected to be exploited to extract more information involved in the Z-curves.
  Xinmin Zhou , Lina Tan and Li Pan
  A robust text watermarking algorithm is proposed to solve the problem of copyright protection for Chinese text documents in this study which watermarking structure and embedding strategy are comprehensively considered. Structure types and strokes of Chinese characters can be obtained through Chinese characters mathematic expression and the whole document is divided into two blocks by utilizing structure types of Chinese characters, the position selected to embed is chose by Chinese character’s strokes and the watermarking bit in each block. Watermarking bits with a robust structure is generated by chaos encrypting and hamming checkout coding. Positions of the destroyed watermarking bits can be judged exactly and block-checkout and hamming-checkout are used to recover them. The experimental result indicates that the proposed algorithm is robust and transparent.
  Hua Tang , Xin-Sheng Liu , Yu-Zhen Fang , Li Pan , Zong-Wang Zhang , Peng Zhou , Jian-Liang Lv , Shou-Tian Jiang , Yong-Lu Wang and Yong-Guang Zhang
  The Z-curve is a geometrical tool for visualizing and comparing genomes. Since, the curve contains the information carried by the given sequence, DNA sequences could be analyzed systematically. In this study, the Foot and Mouth Disease Virus (FMDV) was analyzed by the Z-curve Method. This research concludes that all serotypes FMDV have a close consanguinity but each has its own characteristic by contrast, the three South African serotypes have a much closer affinity. FMDV have three A + T-rich regions and two G + C-rich regions. The most variable regions of FMDV lie in protein coding areas. And the non-coding region is very conservative. O serotype FMDV is most variable compared with A and Asia1 serotype. Those O serotype FMDV in buffalo and goat have a mutation trend. Compared with host, geographic location have a grater influence on FMDV mutation. The A serotype FMDV strains occurred in India after 2002 are more stable and have a closer phylogenetic relationships compared with the Asia1 strains which were prevalent before 2002. In general, Z-curve Method shows a widely application prospect in the areas of life sciences. Such as gene sequence, molecular epidemiology, homology, evolution, phylogenetic relationships and genetic diversity analysis.
  Li PAN , Yongguang ZHANG , Yonglu WANG , Jianliang LV , Peng ZHOU , Zhongwang ZHANG , Yuzhen FANG and Shoutian JIANG
  To explore the feasibility of developing a new type of plantderived foot-and-mouth disease virus (FMDV) oral vaccine, the plant seed-specific expression vector p7SBin438/VP1 carrying the VP1 gene of the FMDV strain O/China/99 was constructed and transformed into Agrobacterium tumefaciens strain GV3101. This strain was used for transformation of Arabidopsis thaliana via the floral-dip method. The kanamycin-resistant transgenic plants were selected, and the VP1 gene and protein expressions were confirmed by PCR and sandwich-ELISA, respectively. The ELISA positive plants were further confirmed by Western blot assay, and the VP1 gene in the transgenic progeny was confirmed by PCR. The results showed that the Agrobacterium-mediated floral-dip transformation method could produce transgenic Arabidopsis containing the VP1 gene. Therefore, this protocol provides a successful strategy to deliver viral genes to leguminous plants for genetic studies and for production of low-cost oral vaccines.
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