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Articles by Legesse Adane
Total Records ( 2 ) for Legesse Adane
  Mulugeta Tesemma , Legesse Adane , Yinebeb Tariku , Diriba Muleta and Shiferaw Demise
  The main objective of this study was to isolate compounds from root wood of Moringa stenopetala and evaluate antibacterial activities of the isolated compounds. Crude gradient extracts were obtained from five solvents (petroleum ether, chloroform, acetone, methanol and water) with increasing solvent polarity using cold maceration technique. The in vitro antibacterial activity evaluation of gradient extracts and isolated compounds was done on four different pathogenic bacterial strains (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Salmonella Typhimurium) using agar disc diffusion technique. Among the five crude extracts evaluated, the acetone extract was found to be the most active against the tested strains. Thus, this extract was subsequently subjected to column chromatographic separation which led to isolation of four compounds (MS-1, MS-2, MS-3 and MS-4). The chemical structures of the compounds were found to be cholest-5-en-3-ol, palmitic acid, n-octacosane and oleic acid, respectively, based on physical properties and spectroscopic (IR and NMR) data as well as literature reports. Three of the compounds namely cholest-5-en-3-ol, palmitic acid and oleic acid showed highest activity against E. coli. The observed antibacterial activities of the crude extract and the isolated compounds could justify the traditional use of the plant for the treatment of different bacterial infections. Thus, further test is recommended on large number of bacterial strains to decide their potential as candidates in development of antibacterial drugs.
  Mulugeta Teshome , Legesse Adane and Yinebeb Tariku
  Background and Objective: Different parts of Moringa stenopetala are traditionally used to treat several human diseases and treatment of dirty water. We have revealed the bacterial activity of the root wood of M. stenopetala previously. This study was conducted to investigate the phytochemical constituents of root bark extracts of this plant and evaluating its antibacterial activity. Materials and Methods: The powdered plant material was subjected to extraction using solvent systems such as petroleum ether, chloroform and acetone involving and the maceration technique. After yield calculation, the crude extracts obtained were subjected to phytochemical screening and antibacterial activity tests. The antibacterial activity test was carried on four bacterial strains viz. S. aureus, E. coli, P. aerugenosa and S. thyphimurium. Results: The extraction gave 1.34 (0.54%), 2.26 (0.91%) and 3.80 g (1.54%) crude extracts from n-hexane, chloroform and acetone extracts, respectively. Phytochemical screening tests revealed the presence of alkaloids, saponin, terpenoids, anthraquinones, flavonoids, polyphenols and phytosterols in the various extracts. The extracts of M. stenopetala revealed antibacterial effects against the tested bacterial strains with zone of inhibition 15-25 mm and acetone extract was the most active. Conclusion: The findings of the study indicated that the plant has a great potential as a source of modern antibacterial agents against selected bacterial strains and potential role in disinfection of dirty water.
 
 
 
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