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Articles by Laila A. Nasser
Total Records ( 3 ) for Laila A. Nasser
  Suaad S. Alwakeel and Laila A. Nasser
  The occurrence of harmful aflatoxins from agricultural products varies with geographic location, farming practices and processing. To date, no data was reported from Saudi Arabia on mycotoxin content of nuts and edible seeds. Forty samples of edible nuts and dried seeds were randomly collected from different locations in Al-Riyadh, Saudi Arabia. Fungi were detected by seed-plate and dilutions plate method and were cultured on glucose-Czapek's agar, sucrose-Czapek's agar and starch yeast agar. Purified fungal isolates were identified morphologically. Mycotoxins were extractedusing chloroform and detected by thin layer chromatography. Bacterial analysis was done using total plate count method. There was a predominance of A. niger and A. flavus in all medium types. Aflatoxin B1 (8.5 μg mL-1) was detected in peanuts containing A. flavus. Aflatoxin B1 (1.7 μg mL-1) and B2 (1.7 μg mL-1) was detected in sunflower seeds containing A. terreus. T2 toxin (2.8 mg mL-1) was detected in pumpkinseeds containing Stachybotrys chartarum and DAS (2.4 μg mL-1) was detected in a salted peanut sample containing Trichthecium roseum. Four nut samples showed contamination with bacteria. Turkish pine seeds and American walnut had total plate counts of 12x10. Pakistani pine seeds and Iranian salted pistachio had TPC of 3x10. Listeria monocytogenes was isolated from American walnut samples. Government authorities for food safety consumption should continue to monitor and set appropriate guidelines and information initiatives for public knowledge on the safety of these agricultural products whole year round.
  Suaad S. Alwakeel and Laila A. Nasser
  Paper currency is used in exchange for goods and services and so the circulation of paper currency from one individual to another potentially spreads microorganisms. If pathogenic bacteria contaminate these currencies, the rate of infection and death rate from these infectious agents will continue to rise. This study was conducted to survey the bacterial and fungal contamination of paper money and cellular phones samples in Riyadh, Saudi Arabia in May 2010. Each bill and phone was prepared using standard procedures. Of the 390 currency notes, 282 (72.3%) were contaminated with bacteria which included Aspergillus niger, Aspergillus flavus, Candida spp., Penicillium spp. and Rhizopus spp. and bacteria which included Enterobacter cloacae, Klebsiella ozaenae, Cedecea davisae, Yersinia pseudotuberculosis, Acinetobacter iwoffii, Staphylococcus warneri and Enterobacter agglomerans. All isolated bacterial species were sensitive to ciprofloxacin, gentamicin, ticarcillin, tobramycin and trimethoprim-sulfamethoxazole. The use of commercial disinfectants was only effective against Aspergillus niger and Rhizopus spp. Cellular phones were contaminated with Micrococcus and Staphylococcus species and no fungal species were isolated from the sampled cellular phones. Prevention is the hallmark of reducing morbidity and mortality. An efficient health awareness campaign program should be fully implemented to inform the public of the hazards of contaminated paper currencies and even mobile phones.
  Laila A. Nasser
  Forty five species belonging to nineteen genera of terrestrial fungi were recovered on glucose and cellulose Czapek’s media from one hundred and forty drinking water samples which, were collected from different kids (2 schools), primary (77 schools), mediatory (33 schools) and secondary (28 schools) schools from different regions in Riyadh city, Saudi Arabia. Cellulose Czapek’s (39 species related to 19 genera) was rich than glucose Czapek’s (37 species belonging to 15 genera) with the species diversity. Water samples collected from primary schools were the most contaminant with fungal populations as compared with other tested samples. Both Aspergillus and Penicillium contributed the broadest spectra of the isolated terrestrial fungal species where they were represented by eight and six species, respectively on the media of isolation. The most prevalent isolated species of terrestrial fungi on the two isolation media were; Cladosporium cladosporoides, Trichoderma viride, Curvularia lunata, Aspergillus niger, Emericella nidulans, Mucor circinelloides and Rhizopus rhizopodiformis. The majority of the isolated genera and species were almost similar on both of isolation media although several species were recorded in only one isolation medium.
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