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Articles by L. FENG
Total Records ( 2 ) for L. FENG
  Z.-P. WEN , X.-Q. ZHOU , L. FENG , J. JIANG and Y. LIU
  A 9-week feeding trial was carried out with juvenile Jian carp to study the effect of dietary pantothenic acid (PA) on growth, body composition and intestinal enzyme activities. Semi-purified diets with seven levels (4.0, 15.5, 25.6, 36.1, 45.9, 56.1 and 65.9mg PAkg−1) of supplemental calcium d-pantothenate were fed to Jian carp (13.0±0.0g). PA improved specific growth rate (SGR), protein productive value (PPV), protein efficiency ratio (PER) and lipid production value (LPV) (P<0.05). Fish fed the control diet had significantly lower feed efficiency (FE) than that in any other group (P<0.05). Body protein content increased with increasing PA levels (P<0.05), but moisture, lipid and ash of fish carcasses were negatively related to the graded PA levels (P<0.05). Intestine protein content (IPC), hepatopancreas protein content (HPC) and activity of α-amylase, lipase, trypsin, Na+,K+-ATPase, alkaline phosphatase (AKP) and gamma-glutamyl transpeptidase (γ-GT) were all positively affected by the dietary PA levels (P<0.05), while intestine index (ISI) and hepatopancreas index (HSI) decreased with the increment of supplemental levels of PA (P<0.05). These results suggested that PA could enhance fish growth and intestinal enzyme activities. The dietary PA requirement of juvenile Jian carp, Cyprinus carpio var. Jian (13.0–73.0g), for optimal growth estimated by the broken-line analysis was 23.0 mgPAkg−1 diet.
  L. TANG , G.-X. WANG , J. JIANG , L. FENG , L. YANG , S.-H. LI , S.-Y. KUANG and X.-Q. ZHOU
  An 8-week feeding experiment was conducted to determine the effect of dietary methionine supplementation on intestinal microflora and humoral immune of juvenile Jian carp (initial weight of 9.9±0.0g) reared in indoor flow-through and aerated aquaria. Eight amino acid test diets (350gkg−1 crude protein, CP), using fish meal, soybean-condensed protein and gelatin as intact protein sources supplemented with crystalline amino acids, were formulated to contain graded levels of methionine (0.6–22.0%) at a constant dietary cystine level of 3gkg−1. Each diet was randomly assigned to three aquaria. Growth performance and feed utilization were significantly influenced by the dietary methionine levels (P<0.05). Maximum weight gain, feed intake occurred at 12gkg−1 dietary methionine (P<0.05). Methionine supplementation improved hepatopancreas and intestine weight, hepatosomatic and intestine index, intestinal γ-glutamyltransferase and creatine kinase activity, Lactobacillus count, Bacillus count, lysozyme activities, lectin potency, sim-immunoglobulin M content, addiment C3,C4 contents and serum total iron-binding capacity and declined Escherichia coli and Aeromonas counts. Quadratic regression analysis of weight gain against dietary methionine levels indicated that the optimal dietary methionine requirement for maximum growth of juvenile Jian carp is 12gkg−1 of the dry diet in the presence of 3gkg−1 cystine.
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