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Articles by Kun Yang
Total Records ( 2 ) for Kun Yang
  Jianbo Yang , Jia Yao , Kun Yang , Zichun Hua and Jie Yang
  The gene sequence of Decorsin which is extracted from a kind of North American leeches was synthesized. Two recombinant proteins, Annexin V plus Decorsin (AnnV-D39) and Annexin V plus the carboxyl terminal 27 amino acid residues of Decorsin(AnnV-D27), were constructed. And a 10 amino acids linker peptide of GGGGSGGGGS was inserted between Annexin V and Decorsin in AnnV-D39. Using pET-28(a+) as an expressing vector, both two recombinant proteins were expressed in E. Coli BL21(DE3) with high efficiency as inclusion bodies. The expression products were purified by DEAE-Cellulose 52 and Sepharose CL-4B chromatography under denaturing condition. Platelet Aggregation Assay (PAA) shows that AnnV-D39 has good anti-platelet aggregation activity. However, AnnV-D27 shows no such activities in any PAA test. AnnV-D39 shows good anti-platelet aggregation activity as a new antagonist of fibrinogen receptor, while Annv-D27 needs re-modification.
  Hamada Hassan , Mingtao Fan , Tingjing Zhang and Kun Yang
  Total phenolics and flavonoids contents in Chinese wild rice were predicted using Near Infrared (NIR) spectroscopy as a rapid method. A Partial Least Square (PLS) algorithm was applied to perform the calibration. The models were calibrated by cross-validation and the chosen number of PLS factor was achieved according to the lowest Root Mean Square Error Cross-Validation (RMSECV) in calibration set. The correlation coefficient (R) and Root Mean Square of Error Prediction (RMSEP) in the test set were used as the evaluation parameters for the optimal model as follows: R = 0.985; RMSEP = 2.41 and the Residual Predictive Deviation (RPD) = 6.06 for total phenolics contents prediction by Multiplication Scatter Correction (MSC) model. For flavonoids contents prediction, R = 0.978, RMSEP = 1.23 and RPD = 4.81 by non preprocessing model. It could be concluded that NIR spectroscopy has significant potential in the nondestructive determination of total phenolics and flavonoids.
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