Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the
coverage of the most important and influential journals
to meet the needs of the global scientific community.
The fungal flora with special attention to Trichoderma
in 23 soil samples collected from Al-Jabal Al-Akhdar Region, Libya was studied
using different culture media. Trichoderma Selective Medium (TSM), Martin's
medium (MT) and Potato Dextrose Agar (PDA) were the superior media for isolating
Trichoderma. Martin's medium (MT) and Malt Extract medium (ME) were the
most valuable for isolating the greatest number of total fungal count. Trichoderma
Selective Medium (TSM) supplemented with 100 μg mL-1 PCNB was
the most effective medium for counting Trichoderma and recorded 120-140%
efficacy of re-isolation. Trichoderma occurred in moderate frequency
in the tested soil and was isolated from 5 soil samples. Trichoderma counted
0.5-1x103 CFU g-1 dry soil and five Trichoderma isolates
were identified as Trichoderma harzianum. Aspergillus and
Penicillium sp. were the most frequent fungi isolated from the tested soil
and were averaged 8.3-5.5 CFU mg-1 soil, respectively. Further studies
are needed to clarify the distribution of fungal flora especially Trichoderma
sp. in the Libyan soil.
