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Articles by Khalid Abd El Ghany
Total Records ( 1 ) for Khalid Abd El Ghany
  Khalid Abd El Ghany , Ragaa A. Hamouda , Hoda Mahrous , Ekbal Abd Elhafez , Fath Allah H. Ahmed and Hanafy A. Hamza
  Background: Lactic Acid Bacteria (LAB) have great therapeutic and nutritional benefits. Among these are: Improved nutritional value of food, control of intestinal infections, improved digestion of lactose, immune modulator effect, control of some types of cancer and control of serum glucose level. Objective: The aim of this study was carried out to evaluate the therapeutic effect of β-glucan extracted from LAB was isolated from different food sources in Egypt. Materials and Methods: The isolation was performed from local Egyptian boza, cider, cheese and yoghurt. Only seven species from 27 was identified as LAB. Only one showed to produce β-glucan and identified by VITEC® 2 as Pediococcus parvulus . The optimization of isolate growth was examined in different temperature, pH and media. Results: The study revealed that the optimum temperature was 37°C, pH was 6 and the most selective medium for growth was Pediococcus selective medium (PSM). Beta-glucan production was assessed by FT-IR analysis, HPLC and HNMR spectroscopy. The therapeutic effect of Pediococcus parvulus β-glucan as antioxidant against DPPH was more significant, cholesterol lowering effect of β-glucan gave valuable results compared to simvastatin drug, the antibacterial effect and control of cancer in vitro against Ehrlich Ascites Carcinoma (EAC) were also gave valuable results. Conclusion: The extracted β-glucan from Egyptian Pediococcus parvulus had different therapeutic effect in vitro including, antioxidative properties, antimicrobial, control of cancer and cholesterol lowering effect.
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