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Articles by K.W. Chen
Total Records ( 3 ) for K.W. Chen
  X.P. Liu , K.W. Chen , K.H. Wang , J.Y. Wang , G.J. Dai , X.Y. Zhang and G.B. Chang
  Xiaoshan chicken is a well-known breed in China. It has good meat quality and three yellow character. SR92A chicken and Xiaoshan chicken are parents. Sixty five offsprings were reared. DNA Fingerprint (DFP) of parental strains were conducted using one middle-repetitive DNA probe EAV (endogenous avian retroviral). Based on presence or absence of band J (3.48 kbp) in their parents, we carry out 4 mating combinations. -/- for absence of band J in both parents; -/+ for absence in sire, presence in dam; +/- for presence in sire, absence in dam; +/+ for presence in both parents. Quantitative traits recorded for offspring are body weight at 75 days and slaughter traits. The results show that the 75 days weight heterosis generated by -/- type is 74.1 g higher than generated by non-selected populations. We can infer that the allele may be corresponding with the different expression of genes between parents and offsprings. We find band J allele has a bearing on weight heterosis, which will lay a solid foundation for locating weight heterosis QTL.
  Q. Wang , H.F. Li , Y.L. Dai , K.W. Chen , B.L. Li , Z.Y. Wang and J. Zhang
  The study was undertaken to assess dietary CP and ME concentrations for optimum growth performance and carcass characteristics of Gaoyou ducklings. In a 3x3 factorial arrangement, three hundred and sixty zero-day-old Gaoyou ducklings were randomly assigned to experimental diets with 11.70, 11.90 and 12.12 MJ of ME kg-1 of diet; each contained 18.0, 19.0 and 20.0% CP, respectively, from 0-28 days of age. Each dietary treatment had 4 replicates. Body weight and feed consumptions were measured at every weekend, from 0-28 days of age and carcass characteristics were evaluated at 28 days of age. The result showed that ducklings on a diet with 11.70 and 11.90 MJ of ME kg-1 at 0-28 days of age exhibited greater BW gain than this diet with 12.12 MJ of ME kg-1 (p<0.01), though BW gain was not different among 11.70 and 11.90 MJ of ME kg-1 of diet. Mean BW gain of birds fed 18.0, 19.0 and 20.0% CP diets was significantly different (p<0.001). Feed intake was influenced by dietary ME levels (p<0.000) also. Feed intake of birds fed 18.0% CP diets was higher than those of birds on 19.0 and 20.0% CP diets (p<0.01). Feed conversion ratios of birds fed on 11.70 and 11.90 MJ of ME kg-1 of diet were better than those fed on 12.12 MJ of ME kg-1 (p<0.001). Feed conversion ratios of birds fed on 18.0% CP diets were better than those fed on 19.0 and 20.0% CP diets. In the study, there were significant interactions between dietary CP and ME on growth performance. There was a direct relationship between dietary ME levels and eviscerated carcass percentage (p<0.014), abdominal fat percentage (p<0.000) and breast weight (p<0.037). But the half-eviscerated carcass percentage, eviscerated carcass percentage, breast and leg meat percentage were not influenced by dietary CP concentration. Thus, diets with 11.70 MJ of ME kg-1 and 18.0% CP were used more efficiently from 0-28 days of age by Gaoyou ducklings.
  Y.F. Zhu , H.F. Li , W. Han , J.T. Shu , W.T. Song , X.Y. Zhang and K.W. Chen
  The objective of this study was to trace the route of avian Mx (myxo-virus resistance) gene evolution and knowledge of its structure and function variations. Mx gene cDNA sequences of six galliform and anseriform avians were used in this study. Datamonkey and DAMBE were used to detect recombination events and test nucleotide substitution saturation. PAML4b were used to test the selective pressure on amino acid sites. Saturation test did not indicate any sign of substitution saturation. Single breakpoint scanning and genetic algorithm scanning by Datamonkey found two breakpoints, located, respectively in 162 and 999 bp and divided the sequences into three nonrecombination split partitions. The selection test of site-specific mode showed that avian Mx gene sequences had suffered positive selection pressure. Likelihood Ratio Test (LTR) suggested M2a and M8 be more advantageous models,and in a total of eight positive sites with >95% posterior probability were identified in three nonrecombination split partitions. These detected positive sites distributed in N-ternimal and GTP-binding domain of Mx protein and might be important candidate marks for improving avian antiviral activity.
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