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Articles by K.F. Chang
Total Records ( 3 ) for K.F. Chang
  S.F. Hwang , H.U. Ahmed , B.D. Gossen , H.R. Kutcher , S.A. Brandt , S.E. Strelkov , K.F. Chang and G.D. Turnbull
  Impact of long-term crop rotations on populations of soilborne pathogens in the genera Fusarium, Pythium and Rhizoctonia and on canola seedling establishment and development was evaluated under controlled conditions. Soil samples were collected from two crop rotation experiments conducted at two sites in Saskatchewan, Canada. A part of the 2006-soil sample of each rotation was sterilized to compare canola seedling growth with and without soilborne pathogens. With 2007-soil, the sterilization treatment was replaced with a fungicide seed treatment (Apron Maxx) to assess the potential to reduce seedling diseases. Populations of Fusarium, Pythium and Rhizoctonia sp. were estimated in the soil of each rotation using dilution plating onto selective media for each fungus. Higher seedling emergence and increased growth of canola were obtained in the sterilized soil or due to seed treatment. Fusarium was the predominant genus followed by Pythium and Rhizoctonia sp. in the soil of both sites. This study suggests that diverse crop in the rotation may reduce the populations of Fusarium, Pythium and Rhizoctonia in the soil and may contribute to improve the overall growth of canola.
  K.F. Chang , S.F. Hwang , A.H. Khadhair , H.U. Ahmed , S.E. Strelkov , M. Deyholos , G.D. Turnbull and J. Feng
  During the summer of 2003 and 2004, a total of 58 isolates of Ascochyta rabiei were collected from chickpea plants grown in southern Alberta, Canada. RAPD analysis of genomic DNA extracted from these isolates was conducted using six short sequence primers (OPA-03, OPA-13, OPB-07, OPC-01, OPC-20 and OPJ-15) and analyzed to establish the genetic relationship and distance between isolates. A total of 75 bands were polymorphic. The 58 isolates were found to belong to five genotypes, indicating that the A. rabiei population of southern Alberta is genetically diverse. No relationship was found between the genotype groupings obtained through RAPD analysis and previously determined pathotype classifications of the same isolates.
  W.J. Li , J. Feng , K.F. Chang , R.L. Conner , S.F. Hwang , S.E. Strelkov , B.D. Gossen and D.L. McLaren
  To identify Quantitative Trait Loci (QTL) controlling root rot resistance of field pea (Pisum sativum L.), 213 Simple Sequence Repeats (SSR) were screened against a population of Recombinant Inbred Lines (RIL) derived from crosses between a moderately resistant cultivar Carman and a susceptible cultivar Reward. Phenotypic data were obtained following inoculation of pea plants with Fusarium avenaceum (Corda ex Fries) Sacc. in field experiments conducted during 2009 and 2010. Linkage analysis based on a single factor ANOVA indicated that four markers were associated with root rot resistance. QTL analysis based on these four markers identified a QTL on Chromosome VII that explained 21.7% of the variance in resistance. The microsatellite markers that are closely linked to this QTL may be useful for stacking QTLs from Carman and other resistance sources to develop cultivars with superior fusarium root rot resistance.
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