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Articles by K. Sri Rama Murthy
Total Records ( 2 ) for K. Sri Rama Murthy
  R. Kondamudi , V. Vijayalakshmi and K. Sri Rama Murthy
  This study was undertaken to evaluate the most suitable concentration of plant growth regulators and perfect explant (node, internode and thin cell layer explants-TCLs) for callus induction and subsequent organogenesis in an endangered medicinal Ceropegia pusilla. The best callus induction was found on the MS medium supplemented with 6-benzylaminopurine (BAP) 13.32 μM L-1 + 2, 4-dichlorophenoxy acetic acid (2,4-D) 0.45 μM L-1 from TCLs. After the initiation of the callus, it was immediately transferred to MS medium supplemented with BAP along with other auxins like 2, 4-D, Indol-3-Acetic Acid (IAA), Indole-3-Butyric Acid (IBA), Naphthalene Acetic Acid (NAA). The regenerative calli were raised on the MS medium supplemented with 1.13 μM L-1 of 2,4-D. Whereas, the organogenic calli was raised on the medium containing 22.7, 40.86, 45.4 μM L-1 of Thidiazuron (TDZ) induced 37.54±0.29, 37.12±0.18 and 34.32±0.17 shoots, respectively. On the media containing BAP 13.32 + IBA 0.49 to 1.23 μM L-1 micro shoots rooted best and 75% of the shoots were survived. The plantlets were established, acclimatized and thrived in green house conditions with 80%. The regeneration protocol developed in this study provides a basis for germplasm conservation and for further investigation of bio active constituents of this medicinal plant.
  K. Sri Rama Murthy and Vivek Babu Kandimalla
  Seeds of Rhynchosia hirta (Andr.) Meikle and Verdc. locally known as Adavi Kandulu in Telugu, used as food by local tribal in the Udayagiri forest of Nellore district of Andhra Pradesh of southern Peninsular India. The mature seed samples were analysed for proximate composition, total (true) seed protein fractions, amino acid composition, fatty acids profiles, mineral and antinutritional factors. The investigated seed samples contained higher amount of crude protein, crude fat, ash and nitrogen free extractives constitute 19.05, 8.73, 4.48 and 48.78 g/100 seed powder, respectively. The calorific value of seed material was 1690.9 kJ/100 g Dm. The essential amino acids, isoleucine, tyrosine and phenylalanine, were present in relatively large quantities. The fatty acid profiles revealed that the seed lipids contained higher concentrations of palmitic and linoleic acids. The seeds are rich in sodium, Phosphorous, calcium, zinc, manganese and iron. Anti-nutritional factors, such as total free phenols, (2.25%) tannins (4.75%), L-DOPA (0.65%), hydrogen cyanide (0.0654%) and phytic acid (1.32%) are, present in variable quantities. This paper presents the analytical data on the chemical composition and nutritional potential of Rhynchosia hirta seeds with regard to its suitability as a regular component in human diet.
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