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Articles by Joko Prastowo
Total Records ( 2 ) for Joko Prastowo
  Joko Prastowo , Kartika Yuga Wulandari , Yudhi Ratna Nugraheni , Dwi Priyowidodo and Ana Sahara
  Background and Objective: Culicoides spp. compose the most studied vector genera and contain the species that is a vector of Leucocytozoon, the parasite that causes leucocytozoonosis. One hundred species of Culicoides spp. inhabit Indonesia and are scattered among 19 provinces, with great diversity in each region. The aim of this research was to study the species diversity and the dominance of Culicoides on layer farms. Methodology: Midges were obtained from layer farms in Sentolo, Kulon Progo (altitude ±78 m); Pakem, Sleman (altitude ±371 m) and Cangkringan, Sleman (altitude ±745 m). Midges were collected using a light trap (New Jersey Standard #2858) for 12 h, from 6 pm-6 am. Identification was performed using dissecting microscope and classified using the identification key of Wirth and Hubert. Results: Eight species were found in this study: Culicoides arakawae, C. huffi, C. oxystoma, C. guttifer, C. sumatrae, C. peregrinus, C. palpifer and C. fulvus. There was diversity in the species collected from the three research areas. Culicoides arakawae was the dominant species found in Sentolo; in Pakem, the most collected species was C. huffi and in Cangkringan, it was C. fulvus. Conclusion: There was a difference in the number of midges collected from the areas with differing topography. The largest collection of Culicoides (270 midges) was obtained from Sentolo subdistrict, Kulon Progo Regency.
  Priyowidodo Dwi , Yudhi Ratna Nugraheni , Eryl Sri Rohayati and Joko Prastowo
  Objective: The objective of this study was to diagnosis canine babesiosis using conventional and molecular method. Materials and Methods: A one-month-old female dog was admitted to Soeparwi Veterinary Hospital in Yogyakarta, Indonesia at 21st September, 2016, with clinical signs and symptoms such as weakness, poor skin turgor, unable to drink and pallor in the conjunctiva and oral mucous membrane. This puppy weighed was 400 g. From physical examination, found ticks all over the skin. This puppy had never received antibiotics and ectoparasitic agents. There were twenty dogs in the owner house and all dogs were infected by ticks. To establish the diagnosis, laboratory examinations were performed such as complete blood count, peripheral blood smear and molecular examination. Polymerase Chain Reaction (PCR) examination was performed using primer 18S rDNA. Results: From blood smear, microscopic examination, parasite Babesia sp., were found inside the red blood cells. Complete blood count examination showed normocytic hypochromic anemia, thrombocytopenia and lymphocytosis. Conclusion: The amplification showed positive results of Babesia sp., DNA with amplification length of 490 bp and babesiosis caused severe infection in dogs especially puppies.
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