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Articles by Jianping Wu
Total Records ( 2 ) for Jianping Wu
  Juan Zhang , Shengguo Zhao , Zhaoming Lei , Xinrong Wang , Jianping Wu and Michael A. Brown
  The Paraoxonase 1 gene (PON1) encodes paraoxonase which hydrolyzes organophosphate pesticides and nerve gasses. In addition, PON1 has been shown to have significant antiatherosclerotic effects and is a major candidate gene associated with the reduction of atherosclerosis. Most of the literature reporting polymorphisms in PON1 has been in humans while less information about the polymorphism of PON1 gene has been reported in mammals, especially in bovine species. Polymorphisms in the PON1 gene were evaluated in Simmental x Chinese Yellow cattle (n = 32) and Longdong cattle (n = 37) using PCR-SSCP in exons 1-9 of the PON1 gene. Results demonstrated polymorphisms in the bovine PON1 gene at exons 4, 6, 8 and 9. DNA sequencing identified specific substitutions in exon 4, 6, 8 and 9 (g.338G>A, g.647C>T, g.896C>T and g.998T>C, respectively). Two genotypes were identified in exon 4 (GG and AG), exon 6 (TT and CT) and exon 9 (TT and CT) while 3 genotypes were identified in exon 8 (CC, CT and TT). The allelic frequency for the G allele was 92.03% and the A allele was 7.97% in exon 4. Allelic frequencies for the T and C alleles were 89.86 and 10.14%, respectively, in exon 6. In exon 8, frequencies for the C and T alleles were 94.93 and 5.07%, respectively. In exon 9, allelic frequencies for the T and C alleles were 88.41 and 11.59%, respectively.
  Jing Wang , Liping Zhang , Jianping Wu , Jianlei Jia and Qiang Ding
  Association between lambing performance (single lamb and twins) and reproductive hormones has been explored. Twenty four ewes divided into 4 groups of each 6 individuals were sampled according to the number of lambs in two breeds (Mongolian ewes and Poll Dorset ewes) blood samples from each individual ewe were collected at the three periods: estrus (day 0) estrus (day 11) and post-partum anoestrus. All the seven hormones concentration of the Poll Dorset ewes was higher than that of the Mongolian ewes in the three periods. And the hormones secretion shown different patterns in two breeds. Five hormones concentrations of ewes with twins were higher than that of ewes with single lamb, the reverse situation found in MT and T concentration even a few of them were statistically significant (p<0.05) at the three periods. Finally, the highly positive and negative significant correlations between hormones were detected, this relationship also shown the differences in two breeds.
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