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Articles by Jianhua Wang
Total Records ( 5 ) for Jianhua Wang
  Jianzhou Shi , Guirong Sun , Yadong Tian , Ruili Han , Guoxi Li , Yanqun Huang , Jianhua Wang and Xiangtao Kang
  In this study, screening and identification differentially displayed genes related to breast blisters (keel cysts) in the “Gushi chickensxAnkao” F2 resource population. Delta Different Display Reverse Transcription-Polymerase Chain Reaction (DDRT-PCR) in combination with Single Strand Conformation Polymorphism (SSCP) is a very sensitive tool for analyzing gene expression of isolated liver tissue from birds. The special primer pair was designed to identify the differential displayed band and a semi-quantitative Reverse Transcription-Polymerase Chain Reaction (RT-PCR) was performed simultaneously. We now are able to directly characterize genetic differences in breast blisters and the healthy birds. The new ESTs was submitted to GenBank (Accession number: GW317166, GW317167, GW317168, GW317169, GW317170, GW317171, GW836603). Several of the differentially expressed fragments were messenger RNA. Six fragments showed interesting homologies to known sequences from the databases. One fragment did not show any homologies to known sequences. This lays a foundation for further study on the mechanism of differential gene expression in breast blisters and the healthy chickens. It is the first time to demonstrate in this study that Tumor Susceptibility Gene 101 (TSG101) is related to breast blisters (keel cysts) of poultry. Furthermore, GW317167 is suggested to contribute to the development of breast blisters (keel cysts), thus may be candidates of new targets of tumor susceptibility genes.
  Haili Li , Yuanying Yu , Rui Gao , Jianhua Wang , Guodong Yang , Zijun Yang , Deanna Baucom and Rebecca Creamer
  The locoweed plant (Astragalus) is a widely distributed toxic plant in many rangeland regions around the world. It is well known that locoweed plants can produce the alkaloid swainsonine which inhibits α-mannosidases and causing neurological poisonings problems through the consumption of locoweed. Locoweed poisoned grazing animal’s exhibit symptoms of locoism. Locoism was caused by locoweed is one of the most destructive disease of rangeland. Recent studies shown that swainsonine was produced by endophytic Undifilum cinereum which was isolated from Astragalus locoweed (Astragalus mollissimus and Astragalus lentiginosus sp.) and responsible for locoism in grazing animals. The toxicosis effect of U. oxytropis fungi on rats is indistinguishable from locoweed toxicosis on rats. The mechanisms of swainsonine underlying U. cinereum and locoweed are poorly understood. To gain a better understanding of the swainsonine biosynthesis in U. cinereum and to facilitate management of locoweed poisoning problems, two-dimensional gel electrophoresis (2-DE) was performed. The 2-DE is a promising tool to study the protein expression profiling and metabolic pathway. To researchers knowledge the present study was the first proteomic reference map using immobilized pH gradients of U. cinereum. To identify proteins in U. cinereum, proteins extracted from mycelial were separated by 2-DE and IEF, digestion and Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS) with an LTQ ion trap mass spectrometer (Thermo Scientific, Waltham, MA). Samples were analyzed by LC-MS/MS and identified using MASCOT MS/MS search in protein databases.
  Jianzhou Shi , Yadong Tian , Jianhua Wang and Xiangtao Kang
  Breast blisters of poultry are usually more of a management problem than an infectious disease problem. The serum biochemical parameters of 90 breast blister chickens and 98 healthy chickens were tested. The determination of 17 kinds serum biochemical parameters including alkaline phosphatase, glucose, high-density lipoprotein, γ-glutamyl transpeptidase, amylase, total protein, glutamic-pyruvic transamineas, creatinine, cholinesterase, lactic dehydrogenase, triglyceride, glutamic-oxalacetic transaminease and so on. Results showed that serum Triglyceride (TG) and Cholinesterase (CHE) were statistically significantly difference between the healthy chickens and he chickens with breast blister (p<0.01). The serum Triglyceride (TG) and Cholinesterase (CHE) content were significantly higher in the chickens with breast blister than the healthy chickens.
  Haili Li , Keling Wang , Limin Lang , Yali Lan , Zihua Hou , Lixian Zhang , Wenhao Zhu , Qi Yang , Qinfan Li and Jianhua Wang
  This study discuses the effect use of feeding Urea Molasses Multinutrient Block (UMMB) as an alternative supplements on the pica symptom of Limousin cattle. Thirty indigenous pica cattle were chosen from Ningnan mountainous district Guyuan City on the basis of similar bodyweight. The Ningnan mountainous district cattle produce was limited by harsh environments and nutritional factors. Especially the forage quality and availability are affecting nutrient intake. Forage was reduced during winter and early spring of the year. A background of these blocks manufacturing process and their effect on pica symptom of cattle are reported. Sixty cattle were randomly divided equally into control and UMMB treatment supplemental groups. Both groups have the same forage except the treatment group was free access to supplement with UMMB. The experiment lasted for 30 days. The content of mineral elements (Se, Zn, Cu, Co, I, Ca, P) in blood in the experiment group were significantly increased contrast with control group (p<0.05) after feeding the UMMB. The activity of Ceruloplasmin (CP), Superoxide Dismutase (SOD) and Lactate Dehydrogenate (LDH) in the serum of experimental group were significantly increased contrast with control group (p<0.05). The content of mineral elements (Se, Zn, Cu, Co) on feather in the experiment group were significantly increased contrast with control group (p<0.05). According to the field observation and stock owner reflect, the pica symptom was disappeared in the treatment group after feeding UMMB 3 days and the intake and drinking have significantly increased. Cattle have the symptom of pica and the hair was coarseness and lackluster before experiment in the experiment group. The symptom of pica was gradually disappeared and the appearances of cattle were dramatically changed with feeding the UMMB. Furthermore, the color of hair was brightness and bushy and the cattle was in good condition in the treatment group after feeding UMMB. But the hair of the cattle in the control group was sparseness and dirty. The symptom of pica was all disappeared which means the UMMB was an effectively in treated cattle pica. So, the UMMB can be as an effective way mineral supplement and treatment in cattle pica.
  Jianhua Wang , Yusuke Shiozawa , Jincheng Wang , Yu Wang , Younghun Jung , Kenneth J. Pienta , Rohit Mehra , Robert Loberg and Russell S. Taichman
  Several reports have recently documented that CXCR7/RDC1 functions as a chemokine receptor for SDF-1/CXCL12, which regulates a spectrum of normal and pathological processes. In this study, the role of CXCR7/RDC1 in prostate cancer (PCa) was explored. Staining of high density tissue microarrays demonstrates that the levels of CXCR7/RDC1 expression increase as the tumors become more aggressive. In vitro and in vivo studies with PCa cell lines suggest that alterations in CXCR7/RDC1 expression are associated with enhanced adhesive and invasive activities in addition to a survival advantage. In addition, it was observed that CXCR7/RDC1 levels are regulated by CXCR4. Among the potential downstream targets of CXCR7/RDC1 are CD44 and cadherin-11, which are likely to contribute to the invasiveness of PCa cells. CXCR7/RDC1 also regulates the expression of the proangiogenic factors interleukin-8 or vascular endothelial growth factor, which are likely to participate in the regulation of tumor angiogenesis. Finally, we found that signaling by CXCR7/RDC1 activates AKT pathways. Together, these data demonstrate a role for CXCR7/RDC1 in PCa metastasis and progression and suggest potential targets for therapeutic intervention.
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