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Articles by Jameel M. Al-Khayri
Total Records ( 4 ) for Jameel M. Al-Khayri
  Jameel M. Al-Khayri and Magda I. Hassan
  Consumers’ awareness and attitude towards GM food vary among different nations and thought to be affected by socio-demographic attributes. The present study was conducted to assess the consumer knowledge about GM food and to determine the level of acceptance of GM products in Saudi Arabia. A consumer questionnaire was designed to generate a demographic profile for participating consumers and assess their perception. Data were collected in Al-Hassa, Eastern Province, based on 420 randomly selected participants. The results indicated that distribution of information about GM food is limited. This was reflected in the percentage of subjects that were familiar with GM food which was only 65%. Though, their knowledge in basic biology was relatively higher, nearly 90%. Differences in knowledge and acceptance about GM food of males and females were not significant but females were slightly more opposed. The study has shown that education, income and age play a crucial role in consumers’ decisions regarding GM food. Overall, only about 46% of the population tested indicated acceptance of GM food; whereas, nearly 54% rejected consuming GM food. The vast majority of the studied subjects preferred GM food labeling. It can be concluded that the commercialization of GM foods may receive some resistance from over half of the Saudi consumers.
  Abdulaziz M. Al-Bahrany and Jameel M. Al-Khayri
  In vitro cultures subjected to salt-stress have been shown to exhibit unique characteristics that are useful for identifying stress status. The objective of this study was to investigate the physiological responses of date palm, Phoenix dactylifera L. cv. Barhee, callus to salinity stress. Callus were cultured on MS medium supplemented with NaCl, KCl, or CaCl2 at 0.8 MPa (-8 bars) equivalent osmotic potential concentrations. The exposure to salt stress resulted in reduction in callus dry weight as compared to the control. Sodium chloride caused the highest reduction in dry weight followed by KCl then CaCl2. In general, callus water content decreased in response to extending exposure durations regardless of the salt type used. Increasing the exposure duration up to 6 days caused increase in proline content compared to the control. Extending the exposure duration of KCl and CaCl2 to 9 days caused reduction in proline content, due to cell death as indicated by culture browning. Exposure to NaCl initially caused increase in Na+ content but at the ninth day, significant reduction in Na+ content was observed. Increasing salt exposure duration caused significant increase in K+ content as compared to the control, up to 3 days of exposure after which the content decreased but remained higher than the control cultures. The Na+/K+ ratio was also significantly affected by the salt type and the exposure duration. This study has enhanced the understanding of the influence of salinity on physiological aspects of date palm cell cultures.
  Abdulaziz M. Al-Bahrany and Jameel M. Al-Khayri
  Cryopreservation, the storage of materials at ultra-cold temperature, is a useful method for long-term storage of a wide variety of plant germplasm including buds, seeds, seed parts, twigs and cell and tissue cultures. The objective of this study was to define the optimal concentrations of key components of the cryoprotectant solution based on sucrose combinations with either dimethyl sulfoxide (DMSO) or glycerol for date palm (Phoenix dactylifera L.) cell suspension. Callus induced from shoot tip explants isolated from young offshoots of cv. Khalas, the most important commercial cultivars in Saudi Arabia, was used to establish cell suspension cultures. The cells were pretreated in sucrose solution and kept in a cryoprotectant solution containing sucrose at a concentration corresponding to that used in the pre-treatments (0.1, 0.25, 0.5, 0.75 and 1 M) and supplemented with either DMSO at 0, 5 and 10% or glycerol at 0, 1 and 2 M. After freezing in liquid nitrogen for 14 day, cell samples were revived and tested for survivability and competency in terms of colony formation, callus re-growth and subsequent somatic embryogenesis. The highest colony formation (21 colonies), greatest callus growth (0.12 g) and highest embryos number (11 embryos) was recovered from cell cryoprotected in 10% DMSO supplemented with 0.75 M sucrose. This study has resulted in cryoprotectant solutions suitable for date palm cells suspension; thus provided the necessary fundamental knowledge for establishing a germplasm bank based on cryopreservation approach for date palm conservation.
  Abdulaziz M. Al-Bahrany and Jameel M. Al-Khayri
  Cottonseeds (Gossypium hirsutum L.) from lines 54027 and 84033, characterized by enhanced salt tolerance, and their parental line DP62 were studied qualitatively and quantitatively to determine fatty acid composition and proximate analysis. Proportions in fatty acid composition were found to be modified as a result of the salt selection-breeding program. Linoleic acid (18:2) was higher in both salt tolerant lines while palmitic acid (16:0) was lower in line 84027. Significantly different unsaturatedisaturated fatty acid ratios, 3.13, 3.20, and 3.59 were obtained from genotype DP62, 84033 and 84027, respectively. Other parameters studied also reflected genotypic variability, including crude protein, crude oil, crude fiber, ash, nitrogen free extract, and carbohydrates content, This research may contribute to establishing a relationship between the unsaturated/saturated fatty acid ratio of a particular genotype and its ability to maintain physiological and biochemical functions under salt stress.
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