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Articles by J.P. Zhou
Total Records ( 2 ) for J.P. Zhou
  Y. Liu , X. Lu , Y.R. Luo , J.P. Zhou , X.Y. Liu , Q. Zhang and Z.J. Yin
  In this study, IRF1 gene was chosen as a candidate gene to evaluate its effect on porcine cytokine traits in serum. A SNP in exon2 was demonstrated by sequencing and PCR-RFLP analysis. Cytokine traits include IFN-γ and IL10 concentrations in serum were measured when the pigs were at 20 and 35 days of age, respectively. The further association analysis between SNP genotype and cytokine traits were conducted in three pig breeds including Large White, Landraces and Songliao Black pig, one Chinese indigenous breed. The results indicated that the SNP of IRF1 gene had highly significant effect on level of IFN-γ (day 20; day 35) in serum (p = 0.0001; p = 0.0001) and ratio of IFN-γ to IL10 (day 35) in serum (p = 0.0083). The study suggested that the IRF1 gene could be regarded as a molecular marker gene for genetic selection of cytokine traits in the further disease resistance breeding.
  Y. Liu , Y.R. Luo , X. Lu , X.T. Qiu , W.X. Fu , J.P. Zhou , X.Y. Liu , Q. Zhang and Z.J. Yin
  In this study, haematological traits, lysozyme concentration and T lymphocyte subpopulation as markers of innate immunity were detected and compared among Landrace, Large White and a chinese indigenous breed, Songliao Black pig. The animals were of the same age and kept under the same environmental conditions to reduce non-genetic variation in immune traits; they were all apparently healthy and were vaccinated by CSF live vaccine at 21 days of age. Except LY%, MO%, CD4CD8% and RDW, the other detected immune traits were significant difference between before (20 days) and after vaccination (35 day). While the values of MCV, MCH, MCHC, MO%, CD4+CD8+% and CD4+CD8+/CD4CD8+ values decreased after vaccination, the others increased. The values of WBC, GR, LY, MO, CD4+CD8% and CD4+CD8+% were significant difference among Large White, Landrace and Songliao Black pig (p<0.05). Our analysis confirms that Songliao Black pig has better innate immune level than Landrace and Large White. The animal resource population was suggested to be appropriate to investigate further the QTL and genes contributing to differences on these innate immune traits in pigs.
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