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Articles by J.H. Lee
Total Records ( 5 ) for J.H. Lee
  H.K. Lim , K. Choi , P.K. Mandal , O. Baatartsogt , C.H. Lee , J.H. Lee and H.B. Kim
  This study was carried out to investigate the differentially expressed genome between S. gallinarum infected and uninfected control in the spleen lymphocytes of Ross broiler chicks using microarray analysis. GeneChip Chicken Genome Array containing 32,773 transcripts corresponding to over 28,000 chicken genes for simultaneous expression was used. The signal intensity of each gene was normalized and expressed in fold change. A large numbers of genes were found with differential expression majority of which are still unknown in chicken genome. Thirty one known genes were found to have differential expression of which, 25 were up-regulated and 5 were down regulated. Majority of the up-regulated genes belong to immune response system viz., IL8, IL1B, IL10, IL18, IL17A, IL15, transferrin, IFNg, TLR2, TNFRSF1b, TNFRSF15 and the down regulated genes were B-FIV, B-LA, SDF1, B-LBI, belonging to MHC-I and II and CD1d. To validate the expression of these genes RT-PCR was done using primers of 12 selected genes′ with total mRNA isolated from spleen lymphocytes which has confirmed the similar pattern of expression of all the genes as in microarray. The findings in this study have lead to the identification of novel genes which may be useful in further studies to understand the patho-physiology of fowl typhoid towards development of diagnostics and therapeutics.
  J.H. Lee , J.Y. Lee , S.B. Yoo and J.W. Jahng
  This study was conducted to examine if intraperitoneal lithium at high dose, sufficient to induce conditioned taste aversion learning, increases expression of Hippocampal Cholinergic Neurostimulating Peptide (HCNP) and choline acetyltransferase (ChAT) in the brain septo-hippocampal system. Quantitative real-time RT-PCR analysis demonstrated that an intraperitoneal injection of lithium chloride (0.15 M, 12 mL kg-1) acutely increases HCNP mRNA levels in the hippocampus. ChAT immunoreactivities in the medial septal nucleus and the hippocampus, which were determined by immunohistochemistry and western blot analysis, also increased with intraperitoneal lithium. These results suggest that intraperitoneal lithium may acutely activate the septo-hippocampal cholinergic system, via increasing expression of HCNP and ChAT. Additionally, we suggest that activation of the septo-hippocampal cholinergic system with increased HCNP expression may be a part of lithium`s action as unconditioned stimulus in conditioned taste aversion learning.
  Y.J. Hwang , J.Y. Lee , J.H. Lee and J.W. Jahng
  We have examined the proteome patterns of the Nucleus Tractus of Solitarius (NTS) of rats shortly after an intraperitoneal injection of lithium chloride at a conventional dose widely used as unconditioned stimulus inducing the formation of conditioned taste aversion. The intraperitoneal lithium induced an up-regulation of A-Kinase Anchoring Protein (AKAP), which binds to cAMP-dependent protein kinase A (PKA) and regulates the phosphorylation of various proteins implicated in synaptic plasticity and memory consolidation. Expressions of cAMP/PKA signaling related genes and proteins in the NTS were analyzed by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and Western blot and phosphorylation of cAMP response element-binding protein (CREB) was increased in the brainstem NTS by the intraperitoneal lithium. These results suggest that up-regulation of AKAP and CREB activation may be involved in the lithium-induced c-fos expression in the rat NTS. In addition, tentative implications of tight junction protein, cadherin protein and acetyl-Co A carboxylase in the lithium-induced synaptic plasticity and memory formation are discussed.
  F.J. Mussini , C.A. Coto , S.D. Goodgame , C. Lu , A.J. Karimi , J.H. Lee and P.W. Waldroup
  The possibility of improving digestibility of nonstarch polysaccharides present in broiler diets by the use of different carbohydrase enzymes appears as an opportunity to enhance feed utilization by the birds. In this study, the effect of a beta-mannanase product on nutrient digestibility in corn-soybean meal diets was investigated. One-day-old chicks received a nutritionally complete corn-soybean meal for 19 days. At that time birds were randomly allocated to four treatments, each of which had six replicates of five birds housed in battery brooders with wire floors. Aliquots of the basal diet were supplemented with four levels of CTCZYME (CTC Bio Inc., Seoul Korea): 0%, 0.025%, 0.05% (recommended level) and 0.10%. Chromic oxide was used as an indigestible marker. Feed was analyzed for gross energy, chromium and amino acid content. After eight days of acclimation to the test diets, birds were sacrificed and ileal contents collected. Analysis of the ileal contents indicated that digestibility of Lys, Met, Thr, Trp, Arg, Leu, Ile, Cys and Val were significantly (p<0.0001) improved in a linear manner for each increment of enzyme. Ileal apparent metabolizable energy increased with each increment of CTCZYME level. These results show that the enzyme improves feed digestibility by making amino acids more available for the bird and increases energy utilization from the feed. These results suggest that lower levels of protein and energy could be used with the same results but further studies are required to estimate potential levels.
  F.J. Mussini , C.A. Coto , S.D. Goodgame , C. Lu , A.J. Karimi , J.H. Lee and P.W. Waldroup
  The effect of a commercial beta-Mannanase enzyme (CTCZYME; CTC Bio Inc., Seoul, Korea) on broiler performance and dry matter output in corn-soybean meal diets was investigated. One hundred and twenty one-day-old male chicks of a commercial broiler strain were fed for 19 d on a nutritionally adequate diet based on corn and soybean meal. At that time birds were randomly allocated to four treatments, each of which had six replicates of five birds each. Aliquots of the basal diet were supplemented with four levels of the CTCZYME: 0%, 0.025%, 0.05% (recommended level) and 0.1%. Chromic oxide was used as an indigestible marker. After eight days of acclimation excreta was collected daily, weighed and dried at 130°C for 24 h to obtain the dry matter output. After seven days of excreta collection the birds were weighed and the experiment was terminated. There were no significant differences for body weight gain, feed conversion or feed intake. The addition of CTCZYME at the inclusion level of 0.05% and 0.1% significantly reduced (p<0.002) the daily dry matter excreta output per bird. Analysis of the excreta showed a reduction of the nitrogen level as the level of CTCZYME increased, indicating an improvement in nitrogen utilization by the bird. Gross energy of the excreta decreased as the inclusion level of the enzyme increased. When the inclusion levels of the enzyme increased, calcium and phosphorus levels increased, possibly due to a concentration effect. Higher levels of chromium in the excreta were observed with the increment of the enzyme, suggesting an improvement on the digestibility of the feed product of the effect of the beta-mannanase. These data indicate that nutrient digestibility is enhanced by the effect of CTCZYME. The reason for the increasing digestibility not affecting the broiler performance may be due to the short time the enzyme was included in the diet. Another possibility is that a change in carcass composition may have occurred; more protein could have been deposited on the carcass instead of fat when the enzyme was included but further studies are required to confirm this assumption. Also, it has to be taken into account that the dietary protein levels provided the needs for the bird and they probably did not need to assimilate the now more available amino acids due to the effect of CTZyme.
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