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Articles by J.H. Cheng
Total Records ( 7 ) for J.H. Cheng
  H.H. Musa , B.C.Li , G.H. Chen and J.H. Cheng
  Cytogenetic analysis always based on examination of chromosome. Before karyotype of an organism including their number, size, shape and internal arrangements, was determined from images taken from cells in metaphase. When banding techniques were developed, the individual chromosomes were identified. In 1960, in-situ hybridization utilized probes labelled with radioisotopes was used. Additionally, spectral karyotyping, multiplex fluorescence in-situ hybridization, comparative genomic hybridization and more recently array comparative genomic hybridization have proven to be useful for the characterization of structural chromosome aberrations found in conventional cytogenetics. In this review, we summarize the developments of chromosome analysis techniques and layout the applications for each technique in different area of science.
  H.H. Musa , G.H. Chen , J.H. Cheng , B.C. Li and D.M. Mekki
  Anka and Rugao chicken breed were reared under the same environment and management. During 12 weeks age the growth rate of Anka breed was found better than Rugao, and similarly within breeds Males were grow faster than females. The results shows that (live weight, carcass weight, dressing out percentage, semi-eviscerated weight, eviscerated weight, breast muscle weight, leg muscle weight, heart weight, liver weight and abdominal fat weight) were significantly (P< 0.01) different in Anka and Rugao breed, however the Abdominal fat % was statistically non significant (P>0.05) between breeds. Males compared to females shows significantly (P>0.01) higher live weight, carcass weight, semi-eviscerated weight, eviscerated weight, breast muscle weight, liver weight and abdominal fat weight within two breeds. However, Leg muscle weight, heart weight were non significantly different (P>0.05). In addition dressing out percentage was significantly (P< 0.05) different between males and females in Anka breed and non significantly in Rugao breed. In Anka chicken breed abdominal fat weight was positively correlated with live weight, carcass weight, breast muscle weight, and percentage of abdominal fat weight; and it was negatively correlated with leg weight. In Rugao breed abdominal fat weight was positively correlated with live weight, carcass weight, breast muscle weight, leg muscle weight and percentage of abdominal fat. However, the percentage of abdominal fat weight was negatively correlated with live weight, carcass weight, breast muscle weight and positively with leg muscle weight and abdominal fat weight.
  G.H. Chen , J.H. Cheng , E.S. Shuiep , W.B. Bao and H. H. Musa
  Total of 120 chickens from Anka and Rugao breed includes (60 individual per breed and 30 individual per sex) were taken as a representative sample and were slaughtered at Jiangsu Poultry Institute, China, and then the carcasses were dissected manually. Water holding capacity, color density, pH and tenderness were estimated from breast muscle. Breeds were found differed significantly (P< 0.05) in color density, pH and tenderness. Regarding to water holding capacity no significant (P>0.05) different were noted between breeds. Tenderness, the values for shear force were significantly (P>0.01) higher in males than females in two chicken breeds. In addition color density, pH and water holding capacity were non significantly difference (P>0.05). In Anka chicken breed color density was positive correlated with pH, tenderness and water holding capacity, and similarly pH was positively related with tenderness. While in Rugao all meat quality traits shows negative correlation with each others, specially tenderness was observed positive correlation with color density.
  H.H. Musa , J.H. Cheng , W.B. Bao , J.T. Shu , B.C. Li and G.H. Chen
  Tissues specimens include (liver, kidney, small intestine and abdominal fat) were excised from lean (Rugao) and fat (Anka) chickens, both breeds were raised under the same conditions. The expression of LDL receptor mRNA in various tissues of genetically fat and lean chickens were determined by semi-quantitative RT-PCR. The level of gene expression was determined as the ratio of integrated peak area for each individual gene PCR product relative to that of the co-amplified ß-actin internal standard. The results shows that the expression of low density lipoprotein receptor mRNA was significantly (p< 0.05) different between abdominal fat and liver tissues, while they were non significantly different between the other tissues. The levels of LDLR1 mRNA expression in intestine, kidney and abdominal fat tissues were none significantly different between genetically fat and lean, while it was significantly (p< 0.05) different in liver. In addition, the levels of LDLR5 mRNA expression in various tissues were non significantly different between genetically fat and lean chickens.
  H.H. Musa , J.H. Cheng , X.S. Wu , H.P. Ju , D.M. Mekki and G.H. Chen
  Present study was focus to compare LDL receptor mRNA expression, total cholesterol, triglyceride, lipoprotien levels and abdominal fat weight in genetically fat and lean chickens. Genetically lean (Rugao) and fat (Anka) chickens were reared in the same environmental condition, at 12 weeks of age samples of liver tissue were collected and abdominal fat weight was determined. Similarly, total cholesterol, triglycerides and high density lipoprotein were assayed using a commercial enzymatic kit, very low density lipoprotein and low density lipoprotein were estimated using the Friedewald equation. Total RNA from liver tissues were isolated using the standard Trizol methods and then total RNA was reverse transcribed by moloney murine Leukemia virus. Semi-quantitative RT-PCR was developed to quantify the levels of LDL receptor mRNA expression. The level of LDL receptor expression was significantly (p< 0.05) difference between lean and fat chicken. In addition, lean and fat chickens were significantly differed on triglyceride, very low density lipoprotein and abdominal fat weight. The expression of LDL receptor mRNA in liver of fat chicken was negatively correlated with abdominal fat weight. However, in lean chicken was negatively correlated with total cholesterol, triglyceride, lipoprotein concentration, abdominal fat weight and percentage of abdominal fat weight. In addition, within two breeds LDL receptor mRNA expression in liver was negatively correlated with low density lipoprotein, abdominal fat weight and percentage of abdominal fat weight.
  H.H. Musa , G.H. Chen , J.H. Cheng , D.M. Mekki , S.H.Xu and J. Huang
  Lipogenesis plays a role in the maintenances of body composition in the growing animals. Lipogensis was regulated in the liver and adipose tissue by the composition of macronutrients in diet. High carbohydrates and little fat was elevated lipogenesis than rich fat and low carbohydrates. Serum samples were preferred because of cholesterol and triglyceride concentration is about 3-5% higher in serum than in EDTA plasma, although no significant serum plasma difference was observed for HDL. There are a variety of methods to measure the lipoprotein classes. All require separation of the classes before they can be measured and recently analysis was performed usingenzymatic kits. Hormonal regulation of lipogenesis has been investigated by measuring hormone levels in the blood as a function of dietary state and by ablating specific endocrine glands in the intact animals, following replacement therapy with specific hormone. Hormone identified in experiments with intact animals was then tested in cell culture systems. Similarly the effect of nutrition on lipoprotein metabolism was reviewed. In the species point of view, the turkey in contrast to chicken seems to be a bird in which muscle growth is more important than adipose tissue growth. This difference in rate of fat deposition in extra-hepatic tissue is due to inherited differences. On the other hands, atherosclerotic plaques were found more severe in the quail selected for high cholesterol than in that selected for low cholesterol.
  Musa H.H. , G.H. Chen , W.B. Bao , X.S. Wu and J.H. Cheng
  Meat quality such as tenderness, color Density (OD), pH and Water Holding Capacity (WHC) were estimated from breast muscle of genetically fat and lean chickens at 12 week of age. Mutation in lipoprotein lipase and apoVLDL-II genes was detected by PCR-SSCP techniques. Agreement of the genotype frequencies with the Hardy-Weinberg equilibrium expectations was tested using a chi-square goodness of -fit test. Lipoprotein lipase gene frequency was significantly different (p<0.01) in Rugao population, whereas in apoVLDL-II gene there are no significantly different between populations. The populations were differed significantly (p<0.01) within two genes. Lipoprotein lipase genotype was significantly (p<0.05) effect water holding capacity and meat tenderness. However, apoVLDL-II genotype was non significantly affected meat quality traits. The results also indicated that the interaction of Lipoprotein lipase and apoVLDL-II genotype was significantly (p<0.01) effected color density, pH and meat tenderness, whereas it was non significantly effects water holding capacity of breast muscle.
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