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Articles by J.B. Milliere
Total Records ( 2 ) for J.B. Milliere
  A. Mbawala , D. Roger , F.N. Tchuenguem Fohouo and J.B. Milliere
  Antibacterial activity of fifteen samples of Ethanol Extracts of Propolis (EEP) prepared from propolis collected in two different geographic regions of Cameroon were investigated. Those antibacterial properties were determined by the well diffusion method on agar medium and by evaluating the Minimal Inhibitory Concentration (MIC) according to the macrodilution method. The activities of EEP were tested against seven strains of bacteria including four gram positive strains and three gram negative strains. All the samples of EEP studied were active only against gram positive bacteria. The most active samples were the EEP1 and EEP12 (p<0.05). Considering the MIC values, the most susceptible strains to the most active EEP tested were Listeria monocytogenes, Staphylococcus aureus and Bacillus subtilis with a MIC≤18.60 μg mL-1, the least susceptible strain was Enterococcus faecalis to the EEP11 with a MIC value equal to 36.20 μg mL-1. Considering the Principal Component Analysis (PCA), the areas of the minor and the major peaks of the phenolic compounds obtained by HPLC showed a relationship with antibacterial activities of the EEP. The EEP antibacterial properties were also linked to their geographic origins.
  A. Mbawala , F.N. Tchuenguem Fohouo , D. Roger and J.B. Milliere
  Fifteen samples of Promax-C, ethanolic extracts of propolis collected from different hives situated in two localities of the Adamaoua Province of Cameroon were tested each against seven strains of bacteria namely Samonella enterica, Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, Listeria monocytogenes, Pseudomonas fluorescens and Bacillus subtilis. The aim of this study was to evaluate the antibacterial activity of those Promax-C samples. Antibacterial activity essays were investigated by the determination of the zones of growth inhibition using the well diffusion method on agar medium and the evaluation of the Minimal Inhibitory Concentration (MIC) using the macrodilution method. All the Promax-C samples were active against the Gram positive bacterial strains except E. faecalis. On the other hand, there was no activity of those samples on the Gram negative bacterial strains studied. Considering the diameter of the inhibitory zones and the MIC values, the susceptibility of bacterial strains to the Promax-C samples decreased as follows: L. monocytogenes>S. aureus>B. subtilis. The most active sample was Promax-C8 from the Martap locality and the most susceptible bacteria was L. monocytogenes. The areas of the minor and major peaks of the phenolic compounds obtained by HPLC analysis were more important for the Promax-C8 sample, showing that the greatest activity of these antimicrobial components was probably linked to their higher contents in the samples.
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