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Articles by J. Stein
Total Records ( 2 ) for J. Stein
  S. Malla , A.M.H. Ibrahim , Y. Yen , W. Berzonsky , K.D. Glover and J. Stein
  Problem statement: Identification of Quantitative Trait Loci (QTLs) in durable resistant genotype is important for marker-assisted breeding. The study was conducted to identify QTLs linked to FHB resistance in ‘Tokai 66’, a Japanese genotype. Approach: A cross was made between Tokai 66 and Jagalene and the single seed descend was used to advance the population. The F2:4 and F2:5 populations were evaluated by artificially inoculating disease in a mist-irrigated nursery in 2006 and 2007. Disease incidence, severity, FDK and DON content were evaluated in the 128 RILs. Map Manager QTX was used to prepare the linkage map and QTL Cartographer 2.0 was used to identify QTLs for FHB responses from the field data respectively. Results: Four QTLs for FHB responses were detected, of which one QTL each for severity and FDK were stable across two years and were located at the 5B and 3BSc, respectively. Both QTLs conferred resistance to FHB. Two unstable QTLs were detected at the 5B and 3D for FDK and DON content, respectively using the field environment. The 5B QTL for FDK contributed to susceptibility, whereas the 3D QTL for DON content contributed to resistance. Conclusion: The 5B QTL associated with resistance to severity and 3B QTL associated with resistance to FDK could be utilized by winter wheat breeding programs selection to enhance FHB resistance.
  P. Gautam and J. Stein
  Systemic Acquired Resistance (SAR) is a mechanism of induced defense in unaffected parts of the plant by inoculating with microorganisms or other stress as an inducer that confers long-lasting protection against a broad spectrum of microorganisms. This experiment tries to detect the presence of SAR to Puccinia sorghi causing common rust in corn. Common rust susceptible corn plants, in the greenhouse condition, were inoculated by Pseudomonas bacteria; Exserohilum turcicum causing northern corn leaf blight (NCLB); toxin produced by Pyrenophora tritici-repentis (PTR), causing tan spot in wheat; Actigard® 50WG, a selective, systemic compound inducing host-plant resistance (AGD) and a control treatment using water as an inducers. Challenger (P. sorghi) was inoculated one week after inducer. Disease assessment was carried out 13th day after inoculation with P. sorghi by enumerating pustules numbers. Percent area leaf death of inducer-injected leaf was 100% in plants injected with PTR toxin and more than 80% in NCLB treatments. In AGD, PST and water injected plants percentage of dead leaf area was below 55% except two plants in Actigard® treated which were above 80%. Unlike previous findings, PTR toxin significantly decreased the pustules number by 70.52% than control. AGD and PST did not develop systemic resistance and developed pustules numbers comparable to control (PST:~105, AGD: ~104, Control: ~138). Based on this experiment, it can be concluded that SAR can be activated in corn, however, widely adapted commercial like Benzothiadiazole may not be efficient against P. sorghi.
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