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Articles by J. Khatiwada
Total Records ( 2 ) for J. Khatiwada
  D.S. Williams , M. Verghese , L.T. Walker , J. Boateng , L.A. Shackelford , M. Guyton , J. Jones , J. Khatiwada and C.B. Chawan
  This study was designed to evaluate the anticarcinogenic effect of Flax Seed Meal (FSM) (10 and 20%) and Flax Seed Oil (FSO) (7 and 14%) on Azoxymethane (AOM)-induced colon tumors in Fisher 344 male rats during initiation (I), promotion (P) and Initiation + Promotion (I+P) stages of carcinogenesis. After an acclimatization period of 1 week, 14 groups of Fisher 344 male weanling rats, 3-4 week old (15 per group) were assigned to 2 control groups fed AIN 93G diet and AIN 93G + 14% soybean oil (SBO) (high fat control). The remaining 12 groups were assigned to 10 and 20% FSM (I, P and I+P) and 7 and 14% FSO (I, P and I+P). All rats received 16 mg kg-1 body weight of AOM at 7 and 8 week of age. At 20 weeks of age all animals were switched to AIN-93 M diets and at 45 week of age all rats were killed by CO2 asphyxiation. Tumor incidence (%) in colon of rats fed C (7 and 14%) was both 100. Tumor incidences for rats fed FSO (7 and 14%) at I, P and I+P were: 100, 100, 61, 60, 58 and 61, respectively and 80, 80, 66.6, 66.6, 66.6 and 31, respectively for rats fed FSM (10 and 20%) at I, P and I+P. Tumors per tumor-bearing ratios for groups fed C; 10 and 20% FSM (I, P and I + P) were 3.86, 1.28, 1.70, 1.75 and 1.0, 0.94, 0.64, respectively. In rats fed C (7 and 14%) and 7 and 14% FSO (I, P and I + P) T/TBR ratios were 3.86, 5.96; 1.4, 0.6, 0.6; 1.90, 0.8, 0.8, respectively. Glutathione-S-Transferase activity (a phase II detoxification enzyme) was significantly (p<0.05) higher in rats fed 10 and 20% FSM and 7 and 14% FSO compared to controls. The results of this study indicate that bioactive phytochemicals such as dietary fiber and lignans such as secoisolariciresinol diglycoside (SDG) found in flax seed meal and essential fatty acids such as α-linolenic acid (ALA) found in flax seed oil suppress colon tumors, particularly at the promotion stage and flax seed products may therefore be effective chemopreventive agents.
  J. Khatiwada , S. Davis and L.L. Williams
  Colorectal cancer is sensitive to dietary intervention. Epidemiological data suggest that high intake of fruits and vegetables may decrease colorectal cancer risk. This experiment was designed to study the preventive effect of green tea catechins and phytic acid in cell culture against the colon tumors. Selected concentrations (0.25 to 25 mM) of green tea catechins and phytic acid were used to treat Caco-2 and HT29 cells maintained in Dulbecco’s modified Eagles Medium (DMEM) with 10% fetal bovine serum. For assay 5x105 cells/well/100 μL were seeded to a 24 well culture plate and incubated at 37°C and 7% CO2 until monolayer was developed, then 400 μL of fresh serum-free DMEM was added to the 24 wells. At the same time selected concentrations (0.25-25 mM) of green tea catechins and phytic acid made up to 100 μL with saline was added and incubated for 24 and 48 h to determine the cytotoxic effect in term of Lactate Dehydrogenase (LDH), Alkaline Phosphatase (AP) and apoptosis assay. The results of this experiment showed that, when Caco-2 and HT29 cells were treated with various concentrations (0.25-25 mM) of green tea catechins and phytic acid for 24 and 48 h, the LDH and AP release was dose and time dependent. When HT29 cells treated with 25 mM concentration of green tea catechins for 24 h and combination of green tea catechins and phytic acid at 5 mM concentration for 12 h showed signs of membrane blebbing which indicates that singly and/or in combination of green tea catechins and phytic acid showed the anti-tumorigenesis activity. Thus, green tea catechins and phytic acid makes it promising chemo-preventive agents of colon cancer.
 
 
 
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