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Articles by J. Safiah
Total Records ( 2 ) for J. Safiah
  A.J. Memon , M. Ikhwanuddin , A.D. Talpur , M.I. Khan , M.O. Fariddudin , J. Safiah and A.B. Abol-Munafi
  The objective of the present study was to evaluate the effects of fresh natural foods on spermatophore quality and analysis by the way of sperm weight, count, viability as well as the proximate analysis of offered food and shrimp broodstock. The experiment was carried out with the three following treatments: fresh squid, polychaete and cockle up to 6 weeks. All parameters were measured by the starting of 3rd week (14 days) and at the end of 6th week (45 days) of the experiment. Spermatophore quality was evaluated by spermatophore weight, sperm count, viability and proximate analysis of the treatments and shrimps. Proximate analysis was performed by method described in AOAC in 1995 and spermatophore count and viability was determined by sperm suspension using modified eosin-nigrosin staining method. At the end of treatments the sperm viability and count, shrimp and spermatophore weight were significantly different among treatments and control (p<0.05). Lipids content in squid was 8.52±0.13% and it was significantly higher by 102.8% than cockle (4.2±0.3%). Whereas, polychaete with 6.86±0.07% which was 63.3% higher than cockle. Also, squid fed to shrimp had the highest level lipids (3.3±0.15%), it was significantly higher about 17.85% as compared to the control (2.8±0.15%). The present study concludes that the fresh squid diet is highly preferred over other diets due to its higher influence on increasing the spermatophore quality, therefore use of fresh squid only is recommended for the maturation of male P. merguiensis broodstock.
  A.J. Memon , A.D. Talpur , M.I. Khan , M.O. Fariddudin , J. Safiah , A.B. Abol-Munafi and M. Ikhwanuddin
  The spermatophore morphology of the P. merguiensis from Kedah, Malaysia is described. About 10 cryopreserved groups as 6, 12 and 24 h, 7, 30, 60, 90, 120, 150 and 180 days and fresh as control was examined from each of three replication were evaluated for sperm gross morphology evaluations. A fully mature male’s broodstock of P. merguiensis was taken with fresh spermatophores and evaluated for sperm morphologically. Cryopreserved spermatophore after the thawing 27°C/2 min (fresh and frozen) individually transferred into glass homogenizer (High speed variable speed reversible, Glas-col, Terre Havte In USA) with 200 μL of Ca-F saline. Fixation, dehydration by series of alcohol, Critical Point Dry (CPD) and mount specimens on to stubs using or carbon dots as well as using Auto Fine Coater and Sputter Coater moreover scanning by Model JEOL 6360LA scanning electron microscope. The cryopreserved spermatophore shows similarities with those of fresh, there were no significant differences (p>0.05) between the freshly spermatophore and spermatophorestored up to 90 days at -196°C liquid nitrogen.
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