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Articles by Iryna Smetanska
Total Records ( 2 ) for Iryna Smetanska
  Nay Min Min Thaw Saw , Heidi Riedel , Onur Kutuk , Kavitha Ravichandran and Iryna Smetanska
  Problem statement: Nowadays, plant cell suspension cultures and immobilized cells are being utilized for the higher yield and quality of the products than extraction of whole plants. Anthocyanins are compounds found in plants that have powerful antioxidant properties. They also provide some of the coloring or pigment of plants, flowers and fruits. Anthocyanin from grape cell cultures can be used as a natural alternative to synthetic dyes, particularly in light of their various health-promoting properties. To enchance the production of anthocyanin in grape (Vitis vinifera) cell culture, the effects of elicitation and precursor feeding on the colorant production were investigated. Approach: In this study, salicylic acid and ethephon were used as elicitors and, phenylalanine and skhikimic acids were used as precursors to improve the productivity of useful metabolites for archiving high concentration in Vitis vinifera suspension cultures. When the cells were elicitated with 50 μL/25 mL suspension salyclic acid, the anthocyanin concentration was increased to 0.03 μg mg-1 in 18 days culture as compared to that in the unelicitated cells. Results: As for precursors, both of the shikimic acid and phenylalanine could promote the synthesis of anthocyanin in the grape cell cultures. After 18 days of the treatment with shikimic acid, it had shown that the anthocyanin concentration in the treatment was 0.05 μg mg-1 more than the control in the cell cultures. Conclusion: In the cell cultures with phenylalanine showed that anthocyanin synthesis was 0.03 μg mg-1 higher than that of without phenylalanine.
  Hoda Bakr Mabrok , Doha Abdou Mohamed , Oksana Sytar and Iryna Smetanska
  Background and Objective: Anthocyanin is responsible for the red color of apple. Ultraviolet light plays a key role in activating the genes responsible for anthocyanin biosynthesis. However, the most important concern is using UV light irradiation on fruit to increase anthocyanins level and its nutritional quality. In this study, the accumulation of anthocyanin in green apple using UV-B and UV-C was investigated and its biological influence was evaluated in rats. Material and Method: Green Golden delicious apples were irradiated with doses of UV-C and UV-B light for a period of 3 h/day each for 3 days. Two Groups of rats were fed on balanced diet or balanced diet supplemented with 10% apple exposure to UV (AP-UV) for a month. Results: The HPTLC and spectrophotometric determination of anthocyanin revealed that color development was significantly increased by 90% in treated apple compared to the control apples. Histological difference was observed between the 2 groups. Plasma levels of uric acid, the activity of transaminases (ALT and AST) as well as malondialdehyde (MDA) were significantly elevated in AP-UV rats. Plasma total cholesterol, triglycerides and creatinine level did not differ among the 2 groups. Liver MDA and catalase levels were eminent in AP-UV rats compared to control. Gene expression of selected inflammatory cytokines (TNF-α, IL-6 and IL-1β) was significantly up-regulated in liver of AP-UV rats in comparison to control rats. Conclusion: The result revealed that there is a health-hazard linked to feeding rats on diet containing irradiated-apple with UV-B and UV-C, which represented by body weight reduction, inflammation development, liver function and oxidative stress elevation.
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