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Articles by Ikram-ul- Haq
Total Records ( 11 ) for Ikram-ul- Haq
  Umar Farooq Awan , Kiran Shafiq , Shazia Mirza , Sikander Ali , Asad-ur- Rehman and Ikram-ul- Haq
  Solid-state fermentation for lipase production was carried by Rhizopus oligosporous ISUUV-16. Almond meal was used as basal substrate with distilled water as moistening agent at substrate to diluent ratio of 1:0.7. Fermentation was carried out at 30oC and culture was incubated for 48 h. Maximum lipase activity under solid-state condition was obtained when Tween 80 at the level of 0.5 % was used as additional carbon source. Best organic nitrogen sources for optimal lipolytic activity was ammonium sulfate (4 %). This study would improve the further optimization steps on the bioprocess development track.
  Asad-ur- Rehman , Sikander Ali and Ikram-ul- Haq
  The effect of different temperatures on citric acid fermentation by Aspergillus niger ANABt was studied in a 15 L stirred fermentor. Ferrocyanide treated (200 ppm) cane molasses medium containing sugar 150 g l-1, was used as the basal fermentation medium. The citric acid yield ranged from 48.58-65.96 g l-1. Maximum amount of anhydrous citric acid obtained during the course of study was 65.96 g l-1 at 30°C. The sugar consumption was 98 g l-1 while mycelial dry weight was 14.55 g l-1. Mycelia were intermediate round pellets.
  Ikram-ul- Haq , Roheena Abdullah , Hamad Ashraf and Athar Hussain Shah
  This study is concerned with the isolation and screening of mould cultures for the biosynthesis of alpha amylase. Forty mould culture were isolated from soil and tested for the production of alpha amylase. Of all the culture tested, the Saccharomyces cerevisiae-20 and Aspergillus niger-34 gave maximum production of alpha amylase. These strains were tested for the screening of culture media and M1 containing yeast extract, peptone, soluble starch ammonium sulphate, CaCl2, MgSO4, FeSO4 in 100 ml of phosphate buffer gave maximum 2 4 4 production of alpha amylase by Saccharomyces cerevisiae. However, Aspergillus niger-34 gave insignificant result. Thus, Saccharomyces cerevisiae -20 was selected for the production of alpha amylase. The production of alpha amylase was reached optimum 72 h after inoculation at pH 5.5.
  Ikram-ul- Haq and Muhammad Umar Dahot
  The establishment of a micro-propagation protocol for banana (Musa sp.) cv Basrai, was carried out by using meristematic stem cuttings, as an explant. Upto 60% micro-propagation efficiency was increased, when organogenesis (MS; 10.0 μM BA; 15.0 μM IAA) was carried out on medium solidified with 3.60 g L-1 phytagel for 3 weeks, while shoot induction and its multiplication (MS; 10.0 μM BA) were obtained on the medium with 1.0 g L-1 phytagel for 10 days and 2.0 g L-1 phytagel for 20 days, respectively. One culture (20.0 μM BA and 4.0 μM NAA or/and 6.0 μM TDZ) somewhat callus formation was observed but later on was proceeded to death, instead of multiplication. The developed plantlets were cultured on MS basal medium supplemented with IBA (0.50 mg L-1) for root induction. Through this method, complete micro-propagated plantlets were obtained within 3 months.
  Sikander Ali , Asad-ur- Rehman , Amna Ehsan , Ikram-ul- Haq and Javed Iqbal
  The present investigation deals with the effect of vegetative inoculum on submerged citric acid fermentation by Aspergillus niger using stirred bioreactor. All fermentations were carried out following growth on 15% raw molasses sugars for 144 h. Ferrocyanide (200 ppm) was used to control the trace metals present in the molasses medium. The maximum production of citric acid was obtained when 1.0% vegetative inoculum was used. The dry cell mass and sugar consumption were 18.5 and 96.55 g l-1, respectively. The mycelia were intermediate round pellets in their morphology. The specific productivity (qp = 0.074±0.02a g g-1 cells h-1) was several folds higher than many other workers.
  Sikander Ali , Asad-ur- Rehman and Ikram-ul- Haq
  The present investigation deals with the time course study of three different mutant strains of Aspergillus niger (IFS-5, IFS-6 & IFS-17) during citric acid production by surface culture technique. The mutant IFS-17 was found to be the best producer of citric acid (76.22 g l-1, 7 days after inoculation) having high production yield coefficients (i.e., Yp/s 0.492 g g-1 & Yp/x 1.992 g g-1). The product is low volume and high cost product. Thus the use of mutant IFS-17 in citric acid fermentation is economically more feasible due to larger citric acid production vs. shorter incubation period.
  Ikram-ul- Haq , Hamid Mukhtar , Zahid Ali and Nadia Riaz
  The present study was conducted on proteases biosynthesis by Penicillium griseoroseum and its application to cheese formation. Thirty fungal strains of Penicillium griseoroseum were screened for the production of protease using solid substrate fermentation. Results indicated that Penicillium griseoroseum HUV-21 gave the best results for the biosynthesis of acidic protease. Different cultural conditions were employed to enhance protease production in the fermented mash. The enzyme synthesis was maximum (61.85 U g-1), when soybean meal was used as substrate. The optimum temperature, incubation period and depth of substrate were found to be 30°C, 72 h and 20 mm, respectively. It was concluded that protease concentration of 6.0 U g-1 was the best for milk clotting.
  Ikram-ul- Haq , Amna Ehsan , Waseem Ahmad Butt and Sikander Ali
  The optimization of cultural condition was studied for the production of xylanase by mutant strain of Aspergillus niger GCBMX-45. Submerged fermentation was carried out in 250 ml Erlenmeyer flasks. Medium containing (%w/v) NaNO3, 0.1; Tween-80, 0.2; NH4Cl, 0.1; KH2PO4, 0.1; MgSO4.7H2O, 0.03; CaCl2.2H2O, 0.1; wheat bran, 2 was used for xylanase production. The enzyme production was maximum (195 U ml-1) at pH 4.5. One percent meat extract was added in medium as an additional organic nitrogen source.
  Ikram-ul- Haq , Kiran Shahzadi , Uzma Hameed , Muhammad Mohsin Javed and M.A. Qadeer
  The present study deals with isolation, screening and optimization of cultural conditions for biosynthesis of cellulases by Trichoderma harzianum using agricultural byproduct. Twenty different strains of Trichoderma harzianum were isolated from different soil samples by serial dilution method. Of all the strains tested, KM07 gave maximum production of cellulases by solid-state fermentation. Different agricultural byproducts such as wheat bran, wheat straw, rice bran, rice husk and soybean meal were tested for the production of cellulases and wheat bran was found to be the best substrate. The production of enzyme was significantly improved as the wheat bran was moistened with mineral salts CMC solution (ratio 1:1). The cultural conditions such as temperature 28°C and pH 6.5 were also optimized. The production of cellulases was maximum 72 h after the inoculation.
  Muhammad Mohsin Javed , Tehmina Saleem Khan and Ikram-ul- Haq
  In the presented research, lipase production was carried out using consortium of Aspergillus niger and Trichoderma viride (simultaneously) in shake flasks. The effect of three factors on the production rate of lipase was studied. The selected three factors were including different concentrations of rapeseed oil (1.0 and 1.5%), pH (5.0 and 6.0) and incubation temperature (25 and 30°C). These factors and their optimized levels were analyzed by using 23 level factorial designs. Rapeseed oil concentration and pH were found to have positive effects on the production and secretion rate of lipase from consortium. However, the effect of pH was being larger than concentration of Rapeseed oil. Temperature was also effective but with minor importance than the other two factors. Results indicated that optimum conditions for the production of lipase from A. niger and T. viride consortium are 30°C with 1.0% Rapeseed oil as carbon source and medium pH 5.0.
  Muhammad Mohsin Javed , Tehmina Saleem Khan and Ikram-ul- Haq
  Solid-state fermentation is the method of choice for generating the industrially important enzymes. Carbon containing compounds in the agricultural wastes are broken down by the microorganisms, which produce the value added commercial enzymes. Thus, the present study describes the extracellular enzymes production like carboxymethyl cellulase (CMC-ase), avicelase, β-glucosidase and xylanase by Aspergillus niger and Trichoderma viride. Agricultural wastes like wheat bran, sugarcane bagasse, corncob, dried grass and rice bran were used under solid-state fermentation conditions. It was found that both the fungal strains have potential to utilize these wastes efficiently as carbon sources and secrete the extracellular proteins possessing hydrolytic ability for the breakdown of these substrates. Culture extract analysis of both strains showed that excreted protein has CMC-ase, avicelase, β-glucosidase and xylanase activity. However, their rate and profile was somehow different, indicating the preference of these strains towards substrate degradability. Additionally, the effect of mineral salts solution with different ratios (v/w) was also investigated and compared with distilled water. Results indicated that Eggins and Pugh minerals salt solution (2:1 ratio) in the presence of corncob as carbon source was best for the bio-synthesis of extracellular hydrolytic enzymes. Moreover, CMC-ase activity 2.24 U mL-1 min-1 in the culture filtrate of T. viride, while avicelase (1.64 U mL-1 min-1), β-glucosidase (4.47 U mL-1 min-1) and xylanase (164.6 U mL-1 min-1) were found to be more active, when A. niger was grown on corncob.
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