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Articles by Ibrahim M.S. Eldeen
Total Records ( 4 ) for Ibrahim M.S. Eldeen
  Ibrahim M.S. Eldeen , Jochen Ringe and Noraznawati Ismail
  Background and Objective: Bruguiera cylindrica is a mangrove tree species. It has various medicinal uses including usage for, lower blood pressure, bleeding, hemorrhage and ulcers. This study aimed to investigate the anti-inflammatory and anti-rheumatoid arthritis effects of the plant. Materials and Methods: Extracts obtained from the leaves and roots of the plant were evaluated for their inhibitory effects against the enzymes: Cyclooxygenase-2, 5-Lipoxygenase and Acetylcholinesterase using the in vitro bioassay models. The GC-MS was used to determine the major active constituents. Synovial fibroblast cells were induced using TNF-α and IL-1 β and MTT cell viability assay was used to determine the cell viability of both, the normal and the induced cells. Results: The DCM leave extract showed the highest inhibitory effects against the 3 enzymes tested. Fraction 6 (the main constituent was 1,2-Benzenedicarboxylic acid), possessed the strong activity against the 3 enzymes with inhibition percentage of 96% (IC50 = 56), 91% (IC50 = 44) and 89% (IC50 = 25) against 5-lipox, AChE and COX-2 enzymes, respectively. Fractions 1 and 3 (containing pyrrolidine related compound) showed inhibitory effects against the three enzymes with inhibition percentages ranging between 89 and 75%. The tested materials showed no cytotoxic effects at the highest concentration used (50 μg mL1) against the normal synovial cells but they possessed activities against the induced RA cell. The results indicated that the activities were dose-dependent. Conclusion: The metabolites obtained from B. cylindrica in this study possessed inhibitory effects on pro-inflammatory enzymes and cell viability of the induced RA cell line. These results provide scientific validation of the bio-efficacy of B. cylindrica as a source of potential bioactive substances for the treatment of inflammatory and arthritis-related diseases. Further study is needed to assess the observed bio-efficacy on an in vivo experiment.
  Ibrahim M.S. Eldeen , Habsah Mohamed , Wen-Nee Tan , Julius Y.F. Siong , Yosie Andriani and Tengku S. Tengku-Muhammad
  This study aimed to investigate inhibitory effects of methanolic root extract of Xylocarpus moluccensis against cyclooxygenase, 5-lipoxygenase and acetyl cholinesterase enzymes using in vitro models. Based on bioassay guided, the extract yielded two fractions A and B. The fractions were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS). The major component of fraction A was identified to be α-guaiene (98.54%). For fraction B (oil), 15 components were identified. The α-guaiene fraction showed strong activities against, AchE (IC50 21 μgmL–1), 5-lipox (IC50 27 μgmL–1) and COX-1 (IC50 43 μgmL–1). It was weaker against COX-2 with IC50 of 84 μgmL–1. The oil possessed strong activities against 5-lipox and AchE with IC50 of 33 and 25 μgmL–1, respectively. However, it was weaker against both the COX enzymes (IC50 values ≥125 μgmL–1). Indomethacin, zileuton and galanthamine were used as positive controls. Concentration responses of the α-guaiene and oil against the enzymes were obtained using 5 concentrations (25-125 μgmL–1). Means were plotted in a graph with error bars representing 95% confidence intervals. For the α-guaiene, the inhibition response increased significantly with the increase of concentrations from 25-100 μgmL–1 against the four tested enzymes. Both the isolates showed remarkable dual inhibitory effects against 5-lipox and AchE enzymes. The results indicated the potential therapeutic effects of the plant in the treatment of inflammatory related ailments and cognitive disorders. Further study is needed to verify mechanism of actions and effective doses. The isolation and the biological activities observed contribute to the novelty of this study.
  Ibrahim M.S. Eldeen , Mohd. A.W. Effendy and Tengku S. Tengku- Muhammad
  Ethno-science is a term used to encompass studies describe local people’s interaction with the natural surroundings. This including ethnoecology and ethnobotany among others. Ethnobotany has been constructed not to be limited only to plant but also to include studies of algae, lichens and fungi. It is strongly linked with taxonomy, pharmacognosy, phytochemistry, ecology and conservation biology. Ethnobotany with no doubt remains the main revenue contributing to both orthodox and traditional medicine. A number of useful drugs have been developed based on ethnobotanical approaches. However, for most of the investigated plant-derived molecules the mechanism of actions are unknown or merely assumed and specific structure activity relationships have rarely been determined. This situation suggest a focus on a new trend of studies to implement polypharmacological approaches on complex herbal mixtures and extracts. This imply that medicinal plant study should be deviated from targeting a single molecule policy in order to produce useful healthcare products. Issues regarding protection and biopiracy have emerged recently as a results of the growing economic focus of biological prospecting based on traditional knowledge. This including concerns about indigenous rights, cultural knowledge and traditional resources. End users of these information are often third parties who have no direct link with the indigenous communities. This situation fuel the need for socio-ethnobotany, a new sub discipline of ethnobotany that deals with the question of how indigenous people can be compensated for sharing their ethnobotanical knowledge. This article aims to highlight issues related to ethnobotany, current challenges and future perspectives.
  Ibrahim M.S. Eldeen , Abdul Hamid , K.C. Wong , M.A. Abdullah , Tengku S. Tengku-Muhammad , H.S. Abdillahi and J. Van Staden
  Background and Objective: The leaf of Homalomena sagittifolia was reported to have anti-inflammatory, antimicrobials, narcotic, violent intoxication and hallucinogen effects. This study highlights isolation, identification and biological activities of two compounds from the leaves of H. sagittifolia. Methodology: Two isolates were investigated for their inhibitory effects against cyclooxygenase and acetylcholinesterase enzymes. They were also tested for antimicrobial effects against five pathogenic bacterial strains using the micro-dilution assay. The structure of the two isolates were elucidated by interpretation of spectroscopic data and previous available reports in literature. They were identified to be trans-phytol (1) and diacylglyceroglycolipid (2). The compounds were investigated for their anti-inflammatory, anticholinergic and antimicrobial effects using the cyclooxygenase, the microplate and the antimicrobial micro-dilution assays, respectively. Results: Compound 2 possessed good activity against both COX-1 (IC50 = 38) and COX-2 (IC50 = 48). The IC50 values observed with the indomethacin were 4.1 and 181 μM against COX-1 and COX-2, respectively. The two compounds also inhibited activity of acetylcholinesterase with an IC50 values of 8.6 μM (2), 24 μM (1) and 3.3 μM (galanthamine). Compound 2 showed remarkable activity against Gram-negative bacteria Pseudomonas stutzeri and Klebsiella pneumoniae with an MIC value of 98 μM. The MIC values recorded for tetracycline were 87 and 175 μM against P. stutzeri and K. pneumonia, respectively. Conclusion: These results indicated the potential pharmacological properties of the leaves of H. sagittifolia and supported the traditional uses of the plant. Further studies are needed to understand its molecular interactions. This may lead to the development of standardized crude drugs and/or nutraceutical agents.
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