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Articles by I.M. AL-Shahwan
Total Records ( 3 ) for I.M. AL-Shahwan
  M.A. AL-Saleh , I.M. Al-Shahwan , O.A. Abdalla and M.A. Amer
  The goal behind this study was the identification, biological and molecular characterization of a virus infecting garden rocket (Eruca sativa Mill) and producing severe symptoms on it in Riyadh region, Saudi Arabia. Mosaic, stunting and deformation were observed on field grown garden rocket plants. Mechanical inoculation of twelve plant species with sap prepared from infected garden rocket plants resulted in infection of four of them. These were E. sativa Mill, Raphanus sativus L., Brassica rapa L. and Chenopodium ammaranticolor Cost and Reyn L. Symptoms on the first three plant species were systemic mosaic, stunting and deformation, whereas symptoms on C. ammaranticolor were local lesions. Brevicoryne brassicae L., transmitted the virus to E. sativa in a non-persistent manner. Electronmicroscopic examination of carbon-coated grids prepared by the leaf dip method revealed flexuous virus particles typical of potyviruses. Enzyme linked immunosorbent assay indicated that samples collected from symptomatic E. sativa plants in both Riyadh and Qassim regions were infected with turnip mosaic potyvirus (TuMV). Positive results were also obtained using a Reverse Transcription-Polymerase Chain Reaction (RT-PCR) method to detect and identify TuMV from nucleic acid extracts of the symptomatic garden rocket plants collected from that area, using a specific oligonucleotide primer for detection of TuMV-CP. Nucleic Acid Spot Hybridization Assay (NASH) using DIG labeled cDNA probe showed high levels of hybridization signal, whereas no hybridization was observed with uninfected tissues. The nucleotide sequence of the CP gene of the Saudi Arabian isolate of TuMV detected in garden rocket and tentatively denoted (TuMV-SA-Ro) was determined to be composed of 862 nucleotides in length.
  M.A. AL-Saleh , I.M. Al-Shahwan , O.A. Abdalla and M.A. Amer
  M.A. AL-Saleh , I.M. AL-Shahwan , M.A. Amer and O.A. Abdalla
  This study aimed at identifying the causal agent inducing virus-like symptoms on radish and lettuce plants in Riyadh Region, Saudi Arabia. Mottling, chlorosis and mosaic symptoms were observed on lettuce (Lactuca sativa Linn.) and radish (Raphanus sativus Linn.) plants in two areas in Riyadh region. Mosaic symptoms were observed on L. sativa, R. sativus, Eruca sativa Mill and Brassica rapa Linn., whereas local lesions were observed on Chenopodium ammaranticolor Coste and Reyn., which were mechanically inoculated with sap from infected radish and lettuce plants. Electron microscopy revealed filamentous flexuous particles typical of potyviruses. The aphid Brevicoryne brassicae transmitted the virus to lettuce and radish in a non-persistent manner. Turnip mosaic virus (TuMV) was the only detected virus by double antibody sandwich-enzyme linked immunosorbent assay among five viruses suspected of such disease symptoms. Amplification of total RNA extracted from infected lettuce and radish plants yielded 1-1.2 kbp using three degenerate primers which were designed for potyvirus group detection and 985 bp complementary DNA (cDNA) fragments using a specific oligonucleotide primer for TuMV detection. Nucleic acid sequence analysis of TuMV-L-SA revealed a range of 85.3 to 90.9% sequence identity with other TuMV isolates obtained from the GenBank. However, the TuMV-Ra-SA revealed a range of 87.7 to 94.1% sequence identity. The sequence similarity between TuMV-L-SA and TuMV-Ra-SA investigated in the current study was 89%. To our knowledge, this is the first report of TuMV on lettuce and radish in Saudi Arabia.
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