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Articles by I.F. Usoh
Total Records ( 2 ) for I.F. Usoh
  I.F. Usoh , E.J. Akpan , E.O. Etim and E.O. Farombi
  The antioxidant actions of 80% ethanolic extract of dried flowers of Hibiscus sabdariffa L. (HSE) on lipid peroxidation (LPO), reduced glutathione (GSH), glutathione-s-transferase (GST), catalase (CAT), superoxide dismutase (SOD) and vitamin C (VITC), were examined using a model of sodium arsenite (SA) - induced oxidative stress in rats. The oral administration of the extracts (200 and 300mg/kg body weight) significantly (P<0.05) decreased by 37% SA - induced malondialdehyde (MDA) formation in liver, suggesting the role of the extract in protection against pro-oxidant induced membrane damage. Pretreatment with the extracts prior to the intra-peritoneal administration of 10mg/kg body weight of SA reduced significantly (by 86%) and induced non-significantly (by 37%) the level of GSH depletion and GST activity respectively in a dose - dependent manner. The extract also attenuated SA - induced reduction in the serum level of VITC as evidenced by a significant (P<0.05) dose - dependent increase (by 60%) in serum VITC level. Pretreatment with the extracts showed a significant (P<0.05) increase in liver and decrease in whole blood activities of SOD (by 369% and 85%) and CAT (by 829% and 58%) respectively, hence revealing the hepatoprotective and antioxidant effectiveness of the extracts. Furthermore, the extracts, evaluated (in vitro) by their capacity of quenching 1, 1-diphenyl - 2-picrylhydraxyl (DPPH) free radical, showed strong scavenging effects on DPPH free radical at concentration of 0.20mg/ml (1C50 = 0.20mg/ml). The extracts at low and high concentrations showed no inhibitory effect on nitric oxide radical. These findings are suggestive of the possible chemopreventive and antioxidative role played by dried flower extract of Hibiscus sabdariffa L.
  E.J. Akpan , O.E. Etim , H.D. Akpan and I.F. Usoh
  The physical and chemical properties of the oil extracted from six (6) varieties of coconut fruits were determined. The oils were brownish, some yellowish when melted, and at times white when solidified. The oils had specific gravity ranging between 0.90 and 0.92. The yield of the oil (% contents) from the ether extract was also analyzed using soxhlet extraction technique. The results showed the oil contents of 88.54% in dry samples and 69.14% in fresh samples. Result of the findings showed a higher percentage of saturated fatty acid content across the entire samples (88.5±0.85% - 97.0±0.37%) in the dry samples, than the unsaturated fatty acid constituents (8.4±0.45% - 9.5±0.30%). The moisture content was higher in the fresh samples than the dry samples. These findings therefore suggest a high percentage oil yield in the dry samples. It is therefore advisable that the nuts be harvested dry if the oil is intended for commercial use.
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