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Articles by I. Hussain
Total Records ( 7 ) for I. Hussain
  M.A. Ilyas , I. Hussain , M. Siddique and M.S. Mahmood
  An inactivated vaccine was prepared from local isolates of egg drop syndrome (EDS) virus using aluminum hydroxide as an adjuvant. A total of 30 commercial layers at the age of 16 weeks were procured and divided in to two groups, A and B having 20 and 10 birds, respectively. The birds of group A were vaccinated with 0.5ml aluminium hydroxide gel adsorbed EDS vaccine. The birds of group B were kept as unvaccinated control. The serum samples were collected at a 14-day interval till 6 weeks post vaccination. At 22 weeks of age group A was again divided into two groups A1 and A2. Birds of group A2 and group B were challenged with a virulent EDS virus. The results revealed that birds of group A had HI titer ranging from 1:128 to 1:512 at different times of sample collection with a geometric mean titer 116. Highest antibody response appeared on 4th week post vaccination. Results also revealed that the vaccination protected the birds against challenge with the virulent EDS virus. Where there was a significant drop in egg production in unvaccinated challenged birds. The egg quality was also deteriorated as miss-shapened, soft-shelled and shell-less eggs were laid in EDS unvaccinated layers.
  M. Sajjad , S.U. Rahman , I. Hussain and M.H. Rasool
  Pathogenic E. coli was isolated from the birds suffering from colibacillosis showing characteristic lesions. Two types of coliphages T1 and T4 were isolated from the sewage water collected from different areas of Faisalabad, Pakistan. These phages were separately tested for in vitro lysis of their host cell on tryptone agar plates, with equal (1:1)) concentration (107 CFU and 107 PFU) of E. coli and phages, which resulted in complete absence of colonies of bacteria after 10 minutes. When phage concentration was reduced (1:½ and 1::¼), colonies continued to appear even after 10 minutes. Later these phages were mixed and evaluated through in vivo trials in broiler chickens of two weeks age. A total of 100 broilers were divided into four groups (A, B, C and D) having 25 birds in each group. First three groups were administered with equal (1:1) concentration of phages as well as E. coli (107/ml) through oral, intraperitoneal and intramuscular routes. The control group (D) was further subdivided into two groups of D1 and D2, both of which were deprived of phages and E. coli respectively. The 13 birds of group D2 that were given only the phages did show neither the signs of disease nor mortality. The results indicated a good protection level using coliphages when compared with the control group. It was concluded that phages may be used effectively as good alternatives of those antibiotics to which bacteria have become resistant.
  M.S. Mahmood , M. Siddique and I. Hussain
  Isolation of infectious bronchitis virus (IBV) was conducted in asymptomatic layer flocks with a history of IB or IB vaccination at least from 5 to 20 weeks before the day of sampling. A trypsin-induced hemagglutination (THA) assay to detect IBV in allantoic fluid of embryonated eggs was used. Detection of IBV through THA showed that group A [5 wks post-inoculation (PI)] was 100%, group B (10 wks PI) was 86%, group C (15 wks PI) was 74% and group D (20 wks PI) was 28% positive for IBV. Virus titre was found negatively correlated with the PI duration.
  Masood Akhtar , M. Mazhar Ayaz , C.S. Hayat , Abdul Jabbar , M. A. Hafeez , M. Ashfaque and I. Hussain
  The involvement of secretary immunoglobulin system in the protection of mucous surfaces by locally produced antibodies lead to the demonstration of IgA. IgA in the intestine of immune birds may limit coccidian replication. Enzyme linked immunospot (ELISPOT) assay was employed to detect the mucosal immune response (IgA antibody secreting splenic cells) in chicken vaccinated with egg adapted gametocytes antigen. Twenty to twenty-five % (200,000 to 250,000/106 cells) of chicken splenic cells stimulated with gametocytes antigen were detected as IgA secretors. Results of the ELISPOT revealed that spleen is one of the major source of cells producing IgA in chickens. Such a high number of IgA antibody secreting cells indicate protection in birds vaccinated with gametocytes antigen via oral route against coccidiosis. ELISPOT assay is found to be an advantageous alternative to conventional haemolytic plaque technique, being simpler and of equivalent or even greater sensitivity.
  S. Mahmood , I. Hussain , M. Sarwar , T.H. Shah and L. A. Khan
  The effect of various levels of microbial culture (EM4) on nutrient digestibility and performance was studied in 120 male broilers. The birds using water supplemented with EM4 gained more weight and used their feed more efficiently than those of control group. However, feed consumption remained unaffected. The highest weight gain and the best feed efficiency was observed in the broilers using the lowest amount of EM4 in the water. The mean values being 1370, 1524, 1563 and 1569 g for weight gain and 3.0, 2.68, 2.65, and 2.55 for feed efficiency (g feed/g gain ) in groups A, B, C and D, respectively. Use of EM4 did not show any effect on dressing percentage and on the relative weight ( g/100g body weight ) of liver, heart, pancreas and gizzard indicating no anatomical changes in the internal organs. Digestibility of dry matter, crude protein and crude fibre contents of the rations also remained unaffected.
  Mahmood M.S. , A.N. Ahmed and I. Hussain
  Present study was performed on three hundred and twenty samples of poultry meat and poultry meat products, 40 samples each from fresh poultry meat, fresh chicken boneless, frozen poultry meat, frozen chicken nuggets, frozen chicken burgers, chopping boards, mincing machines and cleaning cloths collected from different poultry meat sale centers, supermarkets and shops at Faisalabad. Listeria species could be isolated from all the examined samples at different percentages ranging from 10 to 37.5%. Listeria monocytogenes (L. monocytogenes) was differentially identified from other Listeria species by colony characteristics, `beta haemolysis`, `cold enrichment procedure`, `Anton test`, `aesculin test`, characteristic `tumbling motility`, sugar fermentation tests and serotyping. L. monocytogenes could be isolated at variable percentages ranging from 2.5 to 17.5% of the examined samples from poultry meat and poultry meat products. From the 31 isolates of L. monocytogenes isolated from examined samples, 23 strains belonged to type 1 and 8 strains belonged to type 4. Study revealed that the incidence of L. monocytogenes was much higher in frozen poultry meat products and other related inanimates as compared to fresh poultry meat samples. The public health hazards as well as suggestive measures to reduce human Listeriosis have been discussed.
  I. Hussain , M. Asif , M. Ahmed , M. Khan and I. Shakir
  A 45 days storage experiment was conducted to investigate the effect of Uni-Packaging treatments on the shelf life of citrus fruits. Different treatments were polyethylene bags of 0.0254mm, 0.0508mm thickness and control. The result showed that the uni-packaging had no significant effect on the pH of citrus fruit. Weight loss increased significantly as storage increased. Maximum weight loss observed in control and minimum weight loss in thick packaging (0.0508mm). The T.S.S increased during storage but individual packaging had non-significant effect on the T.S.S. Ascorbic acid decreased from 1.59-0.63% during storage. The organoleptic properties evaluation revealed that individual packaging had significant effect on the external appearance, taste and texture. Thick packaging perform significant effect in prolonging the shelf life of citrus fruit.
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