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Articles by I Nyoman Pugeg Aryantha
Total Records ( 2 ) for I Nyoman Pugeg Aryantha
  Alfi Rumidatul , Endah Sulistyawati and I Nyoman Pugeg Aryantha
  Background and Objectives: Sengon Falcataria moluccana (F. moluccana) has the ability to synthesize protease inhibitors, such as trypsin inhibitors, that can inhibit the activity of an enzyme produced by Uromycladium tepperianum (U. tepperianum), a fungus that causes gall rust disease. The purpose of this research is to study the activity of the trypsin inhibitor, the total protein content and the protein profile in healthy and gall rust infected sengon. Materials and Methods: The samples used in this research were taken from sengon twigs consisting of healthy and gall rust infected bark and wood. In this research, three age classes of sengon were used: 1-2 years, 3-4 years and 5-6 years. The method used in this research was field sampling, trypsin inhibitor activity analysis, protein determination and protein profile analysis by SDS-PAGE (Sodium dodecyl sulfate-polyacrylamide gel electrophoresis). Results: The results showed that, in general the activity of trypsin inhibitors in the gall rust infected sengon twigs was higher than in the healthy sengon twigs. The highest protein content was found in gall rust infected bark twigs and the lowest protein content was in healthy wood twigs. The results of the SDS-PAGE analysis of healthy and gall rust infected bark showed that there was a protein with a molecular weight of 21 kDa, which was identified as a trypsin inhibitor. Conclusion: Falcataria moluccana infected with U. tepperianum fungus showed an increase in the activity of trypsin inhibitors and protein content.
  Yurnaliza , I Nyoman Pugeg Aryantha , Rizkita Rachmi Esyanti and Agus Susanto
  Ganoderma boninense is a major pathogen in oil palm plantations which easily infects basal stems and thus it is difficult to be eradicated. Fungal endophytes from healthy oil palm tissues can be used as biological control agents in host plants against G. boninense. For selecting the antagonist candidates against G. boninense, a simple method of dual culture assay was used. A total of 526 endophytic fungi were isolated from 531 oil palm segments in three of plantations location in Medan, North Sumatera province of Indonesia i.e. Kuala Bekala (KB), Medan Tuntungan (MT) and Medan Johor (MJ). Overall, fungal isolation rate in oil palm segments was 0.99 and colonization rate was 77.2% and the highest being found in the KB plantation. From different sample parts, we found that palm leaf midrib had the highest value of colonization and isolation rate. Based on six categories in qualitative dual culture assay, 53 isolates showed a high antagonism effect to G. boninense, twenty two of which were found to have a Colony Growth Inhibition (% CGI) value of more than 80%. Further studies showed that all 22 isolates exhibited a chitinolytic activity on Coloidal Chitin Bromcresol purple (CCBp) assay while only ten (crude extract of liquid culture) inhibited mycelial colony of G. boninense. From microscopic observation, fungal endophytes attached themselves to the hyphae of G. boninense at the interaction zone and causing hypae abnormalities to the pathogen. Almost all selected fungal endophytes potential to be biocontrol agents against G. boninense belong to the Genera of Trichoderma.
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