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Articles by Hui Liu
Total Records ( 13 ) for Hui Liu
  Duoyao Cao , Xiaoqi Jing , Xiaoyu Wang , Hui Liu and Dekun Chen
  Staphylococcus aureus is one of the major pathogen which can cause mastitis in dairy animal. CD4+ lymphocytes play a significant role in clearing this type of bacteria. To our knowledge, CD4+ lymphocytes change in mouse model of S. aureus mastitis is poorly understood. To investigate the response of CD4+ lymphocytes in mammary gland, the mice mastitis was induced with S. aureus in this study. New protocol was used to separate lymphocytes in mammary gland. The results showed that CD4+ lymphocytes increased significantly comparing with blank and saline mice (p = 0.0019, p<0.01) at 72 h post Intramammary Inoculation (IMI). At the same time, the infection mice mammary gland tissues were swelled and were suffering from hyperemia. From histological observation, massive inflammatory cells are infiltrated in the tissue. The results suggest that CD4+ lymphocyte is one of important cells in against S. aureus infection in mammary gland.
  Xiaoqi Jing , Duoyao Cao , Hui Liu , Xiaoyu Wang , Xuanduo Zhao and Dekun Chen
  Staphylococcus aureus is a contagious, mastitis-causing pathogen that is highly adapted to survive in mammary gland. This study focuses on γδ T cell populations in mammary gland involved in the immune response against S. aureus. The C57BL/6J mice in 10-15th day of lactation were intramammary challenge with S. aureus isolated from goat chronic mastitis or with PBS (control). γδ T1(interferon (IFN)-γ-producing) cells and γδ T17 (interleukin (IL)-17-producing) cells in the mammary gland during infection were evaluated by cytometry. The results showed that the percentage of γδ T1 in γδ T cells decreased following intramammary injection and significance was observed at 8, 36, 48, 60 and 72 h compared with control. At the same time, expression of IFN-γ mRNA was decreased. However, percentage of γδ T17 in γδ T cells was increased significantly at 18, 48 and 60 h post challenge, accompanied by an increase in the expression folds of IL-17, IL-23p19 and CCR6 mRNA following intramammary injection. Overall, these data suggest that γδ T17 cells, not γδ T1 cells, in mammary gland tissue were activated rapidly and played an important role in mediating mammary inflammation caused by S. aureus.
  Min Xu , Lijuan Zhou and Hui Liu
  There are many communities in the complex network usually and the communities having these overlapping nodes in many occasion. How to detect community structures in complex network with quick and accurate methods become a hot topic in computer science, system science and social science and other related fields. This study makes an intensive study in the complex network and proposes a practical parallel processing algorithm which is based on local modularity to find the overlapping community structures in complex network. In order to test the accuracy and efficiency of the algorithm, a series of experiments have done in different real-world networks and random networks. These experiment data results show that the proposed algorithm can detect the community structures which are overlapping in limited time and work well in practical cases.
  Siwei Peng and Hui Liu
  Due to the anisotropic network, the distribution of nodes is completely random, network topology is not uniform, through the centroid algorithm there are some errors between estimated position and actual position of nodes, "cluster effect" error is prone to made in the positioning process. In order to overcome the positioning error resulting, a modified centroid algorithm is proposed that adds the stage of a correction of node estimated position on the basis of the traditional centroid algorithm positioning completion.
  Xiaoqi Jing , Yansen Han , Duoyao Cao , Shan Mou , Hui Liu , Jing Yao , Lijuan Zhao , Yanqing Zhao , Chuanchang Shang and Dekun Chen
  IL-17 is a crucial mediator of mucosal inflammation for extracellular bacteria clearance. The production of IL-17 was associated with TGF-β and multiple proinflammatary cytokines, especially IL-6 and IL-1β. IL-17 can cooperate additively or synergistically with IL-6 or IL-1β to amplify of inflammatory processes. However, the roles of IL-17 and cytokines associated with IL-17 in dairy goat mastitis is poorly understood. In the present study, the concentrations of IL-17, IL-6, IL-1β and TGF-β were assayed in sera and milk of Guanzhong dairy goat mastitis induced with Escherichia coli using the Enzyme-Linked Immunosorbent Assay (ELISA) at several time points before and after challenge. The results showed that the levels of all cytokines varied slightly in sera while raised significantly in milk post intramammary inoculation with E. coli. The results suggest that IL-17 is one of important mediators in mammary gland inflammation for bacterial clearance in dairy goat mastitis and IL-6 and TGF-β play an important role or help for development in IL-17 producing cells in goat.
  Chun Xiao , Xing-Fei Zhang , Li-Jun Zhang , You-Xin Yuan and Hui Liu
  A new computational method of each component’s weight coefficient in Space-frame is presented based on structure system reliability theory. The key elements are sure which mainly affect structure reliability. The sensor positions are fixed in Spatial Latticed Structure (SLS) healthy monitoring system. Then a distributed safe early-warning system is designed and the related signal processing problem is discussed. The comparison slope law is used to distinguish the type of burr; Lagrange interpolation is applied to make up the missing data packet; data extent block and zero-phase filtering are adopted to rectify the edge distortion and phase shift. Then the accurate status information can be provided for the safe early-warning system. The natatorium’s roof SLS of Wuhan Sports Center is selected as engineering example and all the structures and methods are realized on it.
  Hui Liu , Jin-Lei Tian , Wen Gu , Ying-Ying Kou , Li Feng , Xiao-Fang Ma , Dong-Dong Li , Shi-Ping Yan and Dai-Zheng Liao
  An oxamato-bridged heterobinuclear NiIICuII complex [Ni(cyclam)Cu(opba)]2 · 3DMSO (1) has been synthesized, characterized by elemental analysis, infrared spectra and magnetic susceptibility, where cyclam is 1, 4, 8, 11-tetraazacyclotetradecane and opba is o-phenylenebis(oxamato). The complex crystallizes in the triclinic system, space group P1 with a = 12.006(3) Aring, b = 12.783(3) Aring, c = 20.977(5) Aring, agr = 76.634(4)°, β = 75.172(4)°, γ = 80.818(4)° and Z = 2. According to X-ray crystallographic studies, the four-coordinate copper(II) atom is a slightly distorted planar geometry and is linked to nickel(II) through the exo-cis oxygen atoms of [Cu(opba)]2-; the six-coordinate nickel(II) center lies in a highly distorted octahedral environment. Magnetic susceptibility measurements of the complex in the temperature range 2-300 K indicate that the heterobinuclear NiIICuII units are coupled antiferromagnetically with J = -57.38 cm-1, gNi = 2.25 and gCu = 2.02.
  Ying-Ying Kou , Jin-Lei Tian , Dong-Dong Li , Hui Liu , Wen Gu and Shi-Ping Yan
  A dimeric dichloro-bridged copper(II) complex [Cu2(pdon)2Cl4] · 2DMF (1) and two mononuclear copper(II) complexes [Cu(pdon)(DMSO)Cl2] · DMSO · H2O (2) and [Cu(pdon)3] · (ClO4)2 · 2.25CH3CN · 6H2O (3) (pdon = 1,10-phenanthroline-5,6-dione) have been synthesized and characterized. Variable-temperature magnetic susceptibility studies indicate the existence of weak anti-ferromagnetic coupling in the binuclear complex. The interaction of these complexes with CT-DNA (calf thymus DNA) has been studied using absorption and emission spectral methods. The apparent binding constants (Kapp) for 1, 2 and 3 are 5.20 times 105, 2.68 times 105 and 7.05 times 105 M-1, respectively, showing moderate intercalative binding modes. All of these complexes cleave plasmid DNA to nicked DNA in a sequential manner as the concentration or reaction time is increased. The cleavage mechanism between the complex and plasmid DNA is likely to involve singlet oxygen 1O2 and •OH as reactive oxygen species.
  Rong Li , Fuping Huang , Xiaojun Jiang , Mingyuan Liu , Yanying Song , Hui Liu and Jingyan Zhang
  Catalase-like activity of a dinuclear manganese-salen (Mn-salen) complex, [Mn(salen)(H2O)]2(ClO4)2 (salen = N,N ′ -bis(salicylidene)-1,2-diaminoethane), was investigated. The dinuclear Mn-salen complex exhibits higher catalase-like activity than that of the mononuclear Mn-salen compound, and its activity can be enhanced by an external base. Different reaction intermediates in the presence and absence of an external base were observed, and the catalytically active species was dimeric as evidenced by UV-Vis spectroscopic studies and mass spectrometry data.
  Hui Liu , Ke-Sheng Xin , Yu-Hong Jiang and Zhi-Yong Jiang
  The seroprevalence of Toxoplasma gondii infection in pregnant women was investigated in Shandong Province, China between August 2010 and September 2013. A total of 7956 serum samples collected from 15 representative administrative regions in Shandong Province, China were evaluated by Enzyme-Linked Immunosorbent Assay (ELISA) for the detection of specific antibodies. Overall, 13.33% (1218/9139; 95% CI: 12.63-14.02) of the samples was positive for specific IgG against T. gondii. The highest seroprevalence was found in low educational standards (15.77%; 533/3379; 95% CI: 14.55-17) and followed by secondary educational standards (12.15%; 454/3736; 95% CI: 11.1-13.2) and high educational standards (11.41%; 231/2024; 95% CI: 10.03-12.8). Furthermore, the seroprevalence of T. gondii was the highest in less more 8 weeks (time of pregnancy) (14.12%; 431/3053; 95% CI: 12.88-15.35), followed by 8-12 weeks (13.03%; 508/3900; 95% CI: 11.97-14.08) and >12 weeks (12.76%; 279/2186; 95% CI: 11.36-14.16). The present resulted indicated infection with T. gondii is prevalence in pregnant women in Shandong Province, China which provides relevant “base-line” data for conducting control strategies and measures against toxoplasmosis in this region and elsewhere in China. This is the first report of the comprehensive survey of T. gondii seroprevalence in pregnant women in Shandong Province, China.
  Jeong -Ah Kang , Ying Zhou , Tahlia L. Weis , Hui Liu , Jodie Ulaszek , Nilesh Satgurunathan , Li Zhou , Koen van Besien , John Crispino , Amit Verma , Philip S. Low and Amittha Wickrema
  Erythropoietin and stem cell factor are the key cytokines that regulate early stages of erythroid differentiation. However, it remains undetermined whether additional cytokines also play a role in the differentiation program. Here, we report that osteopontin (OPN) is highly expressed and secreted by erythroblasts during differentiation. We also demonstrate that OPN-deficient human and mouse erythroblasts exhibit defects in F-actin filaments, and addition of exogenous OPN to OPN-deficient erythroblasts restored the F-actin filaments in these cells. Furthermore, our studies demonstrate that OPN contributes to erythroblast proliferation. OPN knock-out male mice exhibit lower hematocrit and hemoglobin levels compared with their wild-type counterparts. We also show that OPN mediates phosphorylation or activation of multiple proteins including Rac-1 GTPase and the actin-binding protein, adducin, in human erythroblasts. In addition, we show that the OPN effects include regulation of intracellular calcium in human erythroblasts. Finally, we demonstrate that human erythroblasts express CD44 and integrins β1 and α4, three known receptors for OPN, and that the integrin β1 receptor is involved in transmitting the proliferative signal. Together these results provide evidence for signal transduction by OPN and contribution to multiple functions during the erythroid differentiation program in human and mouse.
  Song Han , Hong Yi , Shi-Jin Yin , Zong-Yun Chen , Hui Liu , Zhi-Jian Cao , Ying-Liang Wu and Wen-Xin Li
  The potassium channel Kv1.3 is an attractive pharmacological target for immunomodulation of T cell-mediated autoimmune diseases. Potent and selective blockers of Kv1.3 are potential therapeutics for treating these diseases. Here we describe the design of a new peptide inhibitor that is potent and selective for Kv1.3. Three residues (Gly11, Ile28, and Asp33) of a scorpion toxin BmKTX were substituted by Arg11, Thr28, and His33, resulting in a new peptide, named ADWX-1. The ADWX-1 peptide blocked Kv1.3 with picomolar affinity (IC50, 1.89 pM), showing a 100-fold increase in activity compared with the native BmKTX toxin. The ADWX-1 also displayed good selectivity on Kv1.3 over related Kv1.1 and Kv1.2 channels. Furthermore, alanine-scanning mutagenesis was carried out to map the functional residues of ADWX-1 in blocking Kv1.3. Moreover, computational simulation was used to build a structural model of the ADWX-1-Kv1.3 complex. This model suggests that all mutated residues are favorable for both the high potency and selectivity of ADWX-1 toward Kv1.3. While Arg11 of ADWX-1 interacts with Asp386 in Kv1.3, Thr28 and His33 of ADWX-1 locate right above the selectivity filter-S6 linker of Kv1.3. Together, our data indicate that the specific ADWX-1 peptide would be a viable lead in the therapy of T cell-mediated autoimmune diseases, and the successful design of ADWX-1 suggests that rational design based on the structural model of the peptide-channel complex should accelerate the development of diagnostic and therapeutic agents for human channelopathies.
  Sarah J. Coulthurst , Kathryn S. Lilley , Peter E. Hedley , Hui Liu , Ian K. Toth and George P. C. Salmond
  Erwinia carotovora subsp. atroseptica is an enterobacterial phytopathogen causing economically significant soft rot disease. Pathogenesis is mediated by multiple secreted virulence factors, many of which are secreted by the type II (Out) secretion system. DsbA catalyzes the introduction of disulfide bonds into periplasmic and secreted proteins. In this study, the extracellular proteome (secretome) of wild type E. carotovora subsp. atroseptica SCRI1043, and dsbA and out mutants, was analyzed by spectral counting mass spectrometry. This revealed that dsbA inactivation had a huge impact on the secretome and identified diverse DsbA- and Out-dependent secreted proteins, representing known, predicted, and novel candidate virulence factors. Further characterization of the dsbA mutant showed that secreted enzyme activities, motility, production of the quorumsensing signal, and virulence were absent or substantially reduced. The impact of DsbA on secreted virulence factor production was mediated at multiple levels, including impacting on the Out secretion system and the virulence gene regulatory network. Transcriptome analyses revealed that the abundance of a broad, but defined, set of transcripts, including many virulence factors, was altered in the dsbA mutant, identifying a new virulence regulon responsive to extracytoplasmic conditions. In conclusion, DsbA plays a crucial, multifaceted role in the pathogenesis of E. carotovora subsp. atroseptica.
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