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Articles by Hojat Ahmadi
Total Records ( 5 ) for Hojat Ahmadi
  Ali Sharifat Salmani , Seyed Davar Siadat , Mohammad Reza Fallahian , Hojat Ahmadi , Dariush Norouzian , Parichehr Yaghmai and Mohammad Reza Aghasadeghi
  Problem statement: Brucellosis is a globally found infectious disease and there is no licensed vaccine against human brucellosis. The present study carried-out to evaluate the potency of our modified extracted lipopolysaccharide (LPS) of B. abortus to elicit specific anti-Brucella antibodies in animal model (Rabbit) as a part of a candidate vaccine for brucellosis. Lipopolysaccharide is one of the main virulence factors and the most immunogenic structure of smooth strains of Brucella. Approach: Lipopolysaccharide of B. abortus S99 (S-LPS) initially extracted through an optimized method as described previously. After biochemical and pyrogenicity evaluations of the extracted S-LPS humoral immune response against the extracted LPS analyzed in animal model through serological assays such as Rose Bengal assay, Rapid agglutination (Rapid Wright) test and Standard agglutination test (SAT or Wright test) to demonstrate the specific elicited antibodies against the injected LPS. In addition, the interaction of LPS and anti-LPS antibodies was demonstrated by Agarose Gel Immunodiffusion (AGID) assay. Results: Higher doses of B. abortus S99 LPS caused less or equal body temperature increase in comparison to E. coli LPS doses. Sera of immunized animals had been reported positive by RBT because of B. abortus LPS immunogenicity which we extracted through our optimized method. The highest titer of anti-Brucella antibodies detected two weeks after the third immunization (assayed by rapid slide agglutination and standard agglutination tests). Anti-Brucella antibodies of immunized animals reacted more specifically with the LPS of B. abortus in comparison with E. coli LPS and precipitation lines between B. abortus LPS and immune sera appeared after 30 min while detected after three hours for E. coli LPS. Conclusions/Recommendations: The properties of B. abortus S99 LPS concluded from the present study results, suggest the possible use of this component as a carrier or a part of a sub-unit or conjugated vaccine for human brucellosis.
  Nima Rezaei , Asghar Aghamohammadi , Seyed Davar Siadat , Mostafa Moin , Zahra Pourpak , Mehdi Nejati , Hojat Ahmadi , Samineh Kamali , Dariush Norouzian , Bahman Tabaraei and Robert C. Read
  Common variable immunodeficiency (CVID) is a heterogeneous group of disorders characterized by hypogammaglobulinemia and increased susceptibility to recurrent pyogenic infections. This study was performed to subclassify CVID on the basis of the bactericidal antibody responses of patients to polysaccharide meningococcal vaccine. Twenty-five patients with CVID (18 male and 7 female) and 25 healthy volunteers received meningococcal polysaccharide vaccine A + C. Serum bactericidal antibody (SBA) titers were measured at baseline and after 3 weeks. Response was correlated with clinical and immunological manifestations of CVID. Twenty-four (96%) of the 25 normal controls had a protective SBA titer of ≥8 postvaccination, whereas only 16 (64%) of the 25 CVID patients had a protective titer (P value = 0.013). Among the patients with CVID who were nonresponders, there were significantly increased rates of bronchiectasis (P = 0.008), splenomegaly (P = 0.016), and autoimmunity (P = 0.034) in comparison with patients who had protective SBA titers. A reversed CD4/CD8 ratio was more common in the nonresponder group of patients (P = 0.053). We conclude that individuals with CVID who cannot produce protective postvaccination titers after receiving meningococcal polysaccharide vaccine are more likely to exhibit bronchiectasis, splenomegaly, and autoimmune diseases. Vaccination response may define subgroups of patients with CVID, enabling more effective monitoring and therapeutic strategies.
  Payman Salami , Hojat Ahmadi and Alireza Keyhani
  Problem statement: The aim of this research is to determine the energy indices and to make a cost analysis of strawberry grown in open field in Kamyaran zone of Iran. Approach: The data used in the study were obtained from 35 local strawberry growers by using a face-to-face questionnaire in August-September 2009. Results: Total energy input for strawberry production was calculated to be 36822.9 MJ.ha-1. The Energy ratio was 0.48 and energy productivity was found to be 0.25 kg.MJ-1. About 74.5% of the total energy inputs used in strawberry production was non-renewable while only about 25.5% was renewable. The share of 56.6% of the total energy input was depended on the indirect form, whereas 43.4% of the total energy input was in the direct form. Specific energy was 3.96 Economic analyses showed that profit/cost ratio and net profit were 1.49 and 4616.9 $.ha-1, respectively. Conclusion: The net energy in the study area was negative. This means that the amount of output energy is less than input energy and production in this situation is irrational, thus efficient use of resources and proper land management is needed.
  Hojat Ahmadi , Hamzeh Fathollahzadeh and Hossein Mobli
  The physical and mechanical characteristics of Sonnati Salmas apricot fruits, pits and kernels were studied in this study. Technological properties such as dimensions, geometric mean diameter, sphericity, surface area, bulk density, true density, porosity, volume, mass, true density, bulk density, porosity, 1000-unit mass, coefficient of static friction on various surface and rupture force in 3 axes, were determined at 82.34, 16.48 and 13.03% moisture contents for apricot fruits, apricot pits and apricot kernels respectively. Bulk densities of fruits, pit and kernels were 443.2, 539.4 and 540.1 kg/m3, the corresponding true densities were 940.7, 1045.5 and 1023.6 kg/m3 and the corresponding porosities were 52.87, 48.40 and 47.21%, respectively. The volumes, mass and surface area of fruits were larger than those of pits and kernels. Static coefficient of friction of fruit on all surfaces (wood, glass, galvanize sheet and fiber glass sheet) were measured and static coefficient of friction was less bout for pits and kernels on glass and their value were 0.474 and 0.188, respectively. Rupture force of fruit, pit and kernel were 10.11, 497.79 and 18.92 N through length, 7.98, 322.59 and 41.97N through width and 7.01, 337.21 and 99.58 N through thickness. Results showed that rupture force through length were minimum and this result is very important factor in design post harvest machines, especially about apricot pit crasher machine.
  Reza Shapouri , Ashraf Mohabati Mobarez , Hojat Ahmadi , Bahman Tabaraie , Reza Hosseini Doust , Dariush Norozian , Ahmad Zavaran Hosseini and Davar Siadat
  We modified Brucella fermentation medium from FAO/WHO for enhancing of Brucella abortus S99 biomass. The modified media composed of 15 g L-1 peptic digest animal tissue, 15 g L-1 pancreatic digest of casein, 10 g L-1 yeast extract and 0.10 g L-1 sodium bisulphate. Glucose was added during the incubation by fed-batch method (1-30 g L-1). Agitation speed and air flow rates were controlled at 300-500 rpm and 4-8 L min-1, respectively. Cell density was 9-10% and viable count was 3-3.3x1011 mL-1. The modified conditions enhance the biomass production to more 2 times than the FAO/WHO method. Three methods were accomplished for LPS production: Extraction by butanol with enzymatic digestion, hot phenol extraction with enzymatic digestion, modified hot phenol with trichloroacetic acid (TCA) procedure. Yield of LPS extraction was 0.2, 0.8 and 1.3%, respectively. Method III results in a greater yield of LPS which is 6 and 1.5 times the yields of methods I and II, respectively. Protein contamination of LPS was <2, <2 and <2.9% and nucleic acid contamination of LPS was <1, <1 and <1.4%, respectively. The ketodeoxyoctonate content of LPS (in each of the three methods) was in agreement with ketodeoxyoctonate values obtained previously for highly purified LPS of B. abortus. According to present study, hot phenol with trichloroacetic acid (TCA) procedure is the most suitable procedure for large-scale LPS production from Brucellae, which can be employed for the production of Brucella biomass for vaccine and antigen preparations.
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