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by
Helmi. A. Turk |
Total Records (
1 ) for
Helmi. A. Turk |
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Moustafa Y. El-Naggar
,
Mohamed A. Akeila
,
Helmi. A. Turk
,
Ali. A. El-Ebady
and
Mona. Z. Sahaly
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The primary physiological effect of TH4+ and Virkon-S on E. coli serotype O55:
K39 cells in comparison to formalin was investigated. The in vitro activity of the
disinfectants indicated that TH4+ and Virkon-S caused a leakage of sodium (Na),
potassium (K) ions and UV-absorbing materials. The leakage level was directly
proportional to disinfectant concentration and to the exposure time. Virkon-S
recorded higher efflux of Na+ and K+ ions than TH4+. The X-ray microanalysis
for E. coli cells treated with Virkon-S recorded a low elemental level for P, S, K,
Na, Cu, Zn and Mg. TH4+ caused a lowering in Na, K, S, Cl, Fe and P levels,
whereas Cu and Zn levels were similar to the control. On the other hand, TH4+
showed a superior effect on the leakage of UV-absorbing material compared to
Virkon-S. The amount of total protein of E. coli serotype O55: K39 decreased
with the increase of disinfectant concentration. Virkon-S recorded the highest
inhibition for protein synthesis, followed by formalin compared to TH4+. The
electrophoretic profiles demonstrated a considerable difference in the protein
pattern of the treated cells as compared to the control. Formalin treated cells
(0.015 and 0.007%) revealed that the absence of most of the protein bands
between 18.5-36 kDa and all bands above 36 kDa. Virkon-S treated cells (at
0.015%) masked the bands at 36-330 kDa, whereas Virkon-S (0.007%) and TH4+
(0.007 and 0.015%) addition showed a typical protein banding which matched that
of the control. |
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