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Sixteen different Helianthus annuus L. hybrids growing in Turkish Trakya region were included in this study. Three different methods were employed. While germination occurred in the first method following surface sterilization which was done without removing the testa, in the second one surface sterilization was done by removing the testa. Unlike these, however, in the third one, germination occurred in moist cotton media without removing the testa. Culture experiments were done in various media with varying hormone concentrations by using different explants of the obtained samples. Productive results were obtained in cultures in which cotyledon and hypocotyl explants were used. When sections of these explants were investigated, regeneration and callus formation were found to differ between native and hybrid types.
Nuclear DNA content of Trachelium jacquinii subsp.
dalgiciorum, an endemic taxon, was analysed using flow cytometry.
Plants were collected from a natural population. While some plants were
prepared as herbaria material, others were placed in pots and incubated
in growth chamber. Fresh leaves for flow cytometry analysis were chopped
in MgSO4 buffer (with dithiothreitol, propidium iodide and
triton x-100) on ice in a Petri dish and stained with propidium iodide
(with RNase-DNase free). Stained nuclei were analysed on an EPICS XL (Beckmann
Coulter) model flow cytometer. The mean DNA content per plant was based
on 10000 scanned nuclei. Nuclear DNA content (2C-value) of Trachelium
jacquinii subsp. dalgiciorum was found as 3.44±0.14
pg (1C-value 1.72 pg). This study contributes present data on nuclear
DNA content of angiosperm taxa.
