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Articles by Hamid Rashid
Total Records ( 7 ) for Hamid Rashid
  Hamid Rashid , Rizwana Abdul Ghani and Zubeda Chaudhry
  Effects of media, growth regulators and genotype were investigated for callus induction, maintenance and regeneration in wheat (Triticum aestivum L. cvs., Chakwal 86, Rawal 87). The percentage of callus induction in Rawal 87 was 49.58 -75.62% on Murashige and Skoog (MS) medium and 58.31-91.58% on N6 medium. For Chakwal-86, callus induction frequency ranged from 52.08- 81.04% on MS medium and 53.12- 81.02% on N6 medium supplemented with 2mg/l 2, 4 dichlorophenoxy acetic acid(2,4-D) singly, and with three concentrations (0.1, 0.5 and 1mg/L) of 6-Benzylaminopurine (BAP). BAP proved to play no role in callus induction. Sorbitol promoted callus proliferation, on N6 medium supplemented with 2mg/L 2,4-D. For plant regeneration from calli, MS medium with 0.1mg/L IAA and four concentrations (0.5, 1.0, 2.5 and 5mg/L) of BAP were tested. Regeneration frequency was greater (31.9%) in Rawal 87 on the medium containing 0.5mg/L BAP whereas 2.5mg/L of BAP were found suitable for regeneration in Chakwal 86 (15.27%).
  Muhammad Azhar Bashir , Hamid Rashid and Muhammad Akbar Anjum
  Nodal segments, 1.5-3.0 cm long with 1 or 2 nodes taken from in vitro shoot cultures of six jojoba strains, were cultured on MS medium supplemented with 2.5 or 5.0 mg L-1 BA or 2.5 mg L-1 BA + 2.5 mg L-1 of one of NAA, IAA or IBA. Different growth parameters regarding shoot multiplication were recorded. BA at the rate of 2.5 mg L-1 caused the earliest sprouting of buds and produced the longest primary shoot, maximum number of nodes per primary shoot. However, BA at the rate of 5.0 mg L-1 produced the maximum number of shoots per explant. While the combination consisting of 2.5 mg L-1 BA + 2.5 mg L-1 IBA delayed the bud sprouting and produced the minimum number of shoots, the shortest primary shoot and the minimum number of nodes per primary shoot. Among the strains PKJ-3 led the other strains in performance, while PKJ-2 trailed in all parameters. The number of shoots and the length of primary shoot were significantly affected by the interactions between strains and growth regulators combinations.
  Muhammad Azhar Bashir , Muhammad Akbar Anjum and Hamid Rashid
  The study was conducted to initiate roots from in vitro derived shoots of six promising strains of jojoba i.e., PKJ-1 to PKJ-6. MS medium was supplemented with three auxins i.e., IBA, IAA and NAA each at the rate of 1.25, 2.5 and 5.0 mg L-1 in the one experiment and at the rate of 0.5, 1.0 and 1.5 mg L-1 in the other experiment. Different growth parameters pertaining to root formation were recorded. In the 1st experiment, the lowest concentration (1.25 mg L-1) of each auxin gave satisfactory results, the other two higher concentrations of auxins (2.5 and 5.0 mg L-1) caused callus induction. Efficacy of auxins was improved in the 2nd experiment as each auxin and its concentrations affected significantly the root characteristics of each strain. IBA and 0.5 mg L-1 were the most effective auxin and concentration, respectively. Among the strains, PKJ-3 performed the best of all, as it took the minimum time to root, produced maximum number of roots and attained longer primary root and higher percentage of rooted shoots in both experiments.
  Muhammad Ramzan Khan , Hamid Rashid and Azra Quraishi
  Hypocotyl explants from in vitro seedlings were cultured aseptically on Gamborg (B5) medium supplemented with various concentrations and combinations of dichlorophenoxyacetic acid, indoleacetic acid, naphthalene acetic acid and benzylaminopurine for callus formation and shoot regeneration. 2,4-D alone @ 2 mg/l gave the highest callus formation (96%). The results revealed that BAP @ 2 mg/l with IAA 0.5 mg/l was the most appropriate combination to produce the multiple shoots (14.5 shoots per culture). Efficient rooting was achieved on half-strength MS medium supplemented with 0.250mg/I IBA and 0.125mg/l IAA. In vitro raised plantlets were transferred to potted soil and finally to experimental fields to assess their acclimatizing potential.
  Ihsan Ullah , Hamid Rashid and Azra Quraishi
  Three wheat genotypes i.e chakwal-86, NR-58 and Inqilab-91 were tested. Chakwal-86 appeared to be the most responsive genotype to callus induction followed by NR-58 and Inqilab-91. It also produced significantly higher amount of callus compared to other genotypes. Callus induction frequencies varied from 66.65 to 100 percent in Chakwal 86, 49 to 75.80% in Inqilab-91 and 60.90 to 75.50% NR-58 on various levels of 2,4-D (2,4- dichlorophenoxy Acetic Acid). However, medium containing 2 mg of 2,4-D was found to be optimum for callus induction. Regeneration frequency of Chakwal-86 was 33.33% on the medium containing 0.1 mg of IAA (Indole Acetic Acid) and 2.5 mg of BAP (6-Benzyle Amino Purina). While NR-58 and Inqilab-91 showed regeneration percentage of 40 and 25% respectively on medium having 0.1 mg of IAA and 0.5 mg of BAP. Regenerated plants were transferred to free living conditions. Regenerated plants were evaluated for plant height, maturity and seed set. Regenerated plants showed favourable significant differences from control plants at 5 96 confidence level for most of the studied traits; such as plant height, days to maturity and etc.
  Hamid Rashid , Kinya Toriyama , Azra Quraishi , Kokichi Hinata and Kauser Abdulla Malik
  Shoot regeneration from calli of indica rice remains a hinting factor for undertaking transformation experiments. In this study, callus cultures were initiated from seeds of Basmati 370, Basmati 385 and Basmati 6129. A 2,4-0 concentration at 2 mg/I was found to be suitable for callus induction in Basmati 370 and Basmati 385 and was 3 mg/I for Basrnati 6129 in N6 medium. Callus induction frequency ranged from 23.1%-100% for Basmati 370, 73-100% for Basmati 385 and 40-100% for Basmati 6129. Inclusion of Benzyl antiparine (BAP) in callus induction medium has a negative effect for the frequency of callus induction and callus growth. Shoot regeneration was obtained from calti in Basmati varieties between 40%-55.7% in MS medium with 3% sucrose, 3% sorbitol, 2g/I casamino acids, 5 mg/I BAP, 1 mg/I Naphthalie acetic acid (NAA) and 4 g/I gelrite. A maximum shoot regeneration was found in Basmati 370, Regeneration frequency was increased 2-7 folds by the addition of sorbitol and gelrite.
  Hamid Rashid , Mehreen Saleem , Zubeda Chaudhry , S. Tallat Gilani and Asfari Sharif Qureshi
  Super Basmati is a commercial cultivar and is subjected to various stresses. This study was conducted to obtain high frequency regeneration from Super Basmati which is a pre-requisite for transformation protocol. Callus induction was obtained from N6 media with 2 mg l-1 2,4-D. The frequency of callus induction was 83.3% with N6 media and 75.05% on MS media. Regeneration of calli was the best on MS media with NAA 1 mg l-1 and BAP 5 mg l-1 i.e. 81.6%.
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