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Articles by Hamid Reza Monsef-Esfahani
Total Records ( 3 ) for Hamid Reza Monsef-Esfahani
  Abdolhossein Miri , Hamid Reza Monsef-Esfahani , Mohsen Amini , Yaghoub Amanzadeh , Abbas Hadjiakhoondi and Reza Hajiaghaee
  Phytochemical and bioactivity studies of Teucrium persicum Boiss. have been investigated on the aerial parts of the plant. About 2 flavonoids, 5-hydroxy 3, 7, 4-trimethoxyflavone and 5-hydroxy 7, 3′, 4′-trimethoxyflavone were purified and identified from chloroform partition by spectroscopy methods. The crude extracts and isolated compounds were screened for their antioxidant activities using DPPH radical-scavenging, FRAP and Reducing power methods. Methanol extract and isolated flavonoids were found to be the most antioxidant active portions.
  Hamid Reza Monsef-Esfahani , Mohammad Ali Faramarzi , Venus Mortezaee , Mohsen Amini and Mohammad Reza Rouini
  A High-Performance Liquid Chromatography (HPLC) method was developed for determination of harmine, harmaline, harmol and harmalol in the extract of Peganum harmala seeds. The sample preparation was performed using liquid-liquid extraction. Chromatographic separation was achieved with a Tracer Excel 120 ODSA (150x4.6 mm) column, using a mixture of potassium phosphate buffer (10 mM, pH 7.0): acetonitrile (100:30; v/v) as mobile phase, in an isocratic mode at 1.5 mL min-1. UV detection (λ = 330 nm) was used. The calibration curves were linear (r2>0.998) in the concentration range of 0.5-20 μg mL-1 for all analytes. The lower limit of quantification for all analytes was 0.5 μg mL-1. The within and between day precisions in the measurement of QC samples at three tested concentrations were in the range of 0.6-10.2% for all analytes. The HPLC method is able to measure the harmala alkaloids in the plant extract. The method has suitable reproducibility, sensitivity and resolution for routine and accurate use with UV detection.
  Hamid Reza Monsef-Esfahani , Abdolhossein Miri , Mohsen Amini , Yaghoub Amanzadeh , Abbas Hadjiakhoondi , Reza Hajiaghaee and Yousef Ajani
  Essential oils from the aerial parts of Teucrium persicum Boiss. (Labiatae), collected in 3 stages (pre-flowering, flowering and post-flowering) from plants grown in the Fars province of Iran were obtained by steam distillation. The chemical composition of the oils were analyzed by GC-MS. Analysis of the chemical profile of the isolated oils revealed the presence of >80 compounds, mainly sesquiterpene hydrocarbons, oxygenated monoterpenes and sesquiterpenes. They contained α-terpinyl acetate, α-cadinene, 1,4-cadinadiene, linalool and cadinol. Average yield was 1%. The studied essential oils showed good antioxidant activities as calculated by 3 in vitro assays: DPPH radical scavenging, Ferric Reducing Power Assay (FRAP) and Reducing Power (RP).
 
 
 
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