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Articles by Hamdi Uysal
Total Records ( 2 ) for Hamdi Uysal
  Gultekin Yildiz , Pinar Sacakli , Tulin Gungor and Hamdi Uysal
  A study was conducted to determine the effect of, as a source of inulin, Jerusalem artichoke on intestinal pH, some blood parameters and liver enzymes of laying hens. Control and treatment groups were fed a diet containing 0, 5 and 10% Jerusalem artichoke, respectively. Twenty five weeks old, 45 commercial white laying hens were used in the experiment. In this experiment, inulin was effective on modifying of intestinal characteristics, blood metabolites and liver enzymes in laying hens. Fecal and intestinal pH values were not altered by dietary treatments. Although, unchanged serum cholesterol and albumen content, 5% JA increased glucose (p<0.001) and decreased fructose (p<0.01), triglyceride (p<0.01) and total protein (p<0.05) contents when compared with control diet. On the other hand, 10% JA reduced serum glucose as well as fructose levels. Serum SGOT levels was increased (p<0.01) by 5% JA addition and ALP levels was decreased (p<0.05) by 10% JA.
  Hamdi Uysal , Serpil Nalbanto lu , Tevhide Sel , Gulay Ciftci and Cigdem Altinsaat
  To our knowledge, this is the first study in analysing the glycosylated proteins of erythrocytes and lymphocytes of cattle infected with Theileria annulata at high percentage of parasitemia (50-70%). SDS-PAGE separation of extracts of erythrocytes showed that one protein band (28 kDa) was not observed in erythrocytes of bovine theileriosis in comparison to control cells. Interestingly, erythrocytes of bovine theileriosis, analysed by polyacrilamide gels and stained by PAS method, gave much more stronger bands than control erythrocytes although, erythrocytes gave weaker bands on coomassie blue stained polyacrilamide gels in comparison to control erythrocytes. Maackia Amurensis Agglutinin (MAA) lectin staining of semi-dry blotted nitrocelulose membranes after SDS-PAGE analysis of erythrocytes of cattle with theileriosis gave very similar bands with control cells indicating that glycoproteins of erythrocytes of cattle with and without theileriosis possess similar sialic acid structures. Furthermore, stainings of semi-dry blotted nitrocelulose membranes with Dig-glycan detection kit and MAA lectin after SDS-PAGE separation of lymphocytes of cattle with theileriosis gave more prominent glycosylated proteins than control lymphocytes. This result indicates the increased protein glycosylation in lymphocytes of bovine theileriosis when compared with control lymphocytes.
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