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Articles by Hakan IGAN
Total Records ( 2 ) for Hakan IGAN
  Hayrunisa Hanci , Hakan Igan and Muhammet Hamidullah Uyanik
  Background: Many serological tests have been used for the diagnosis of human brucellosis. A new serological method is identified as Brucella Coombs gel test based on the principle of centrifugation gel system similar to the gel system used in blood group determination. In this system, if Brucella antibodies were present in the serum, antigen and antibody would remain as a pink complex on the gel. Otherwise, the pink Brucella antigens would precipitate at the bottom of the gel card system. Objective: In this study, we aimed to compare the Brucella Coombs gel test, a new, rapid screen and titration method for detection of non-agglutinating IgG with the Brucella Coombs test. Materials and Methods: For this study, a total of 88 serum samples were obtained from 45 healthy persons and 43 individuals who had clinical signs and symptoms of brucellosis. For each specimen, Rose Bengal test, standard agglutination test, Coombs test and Brucella Coombs gel test were carried out. Results: Sensitivity and specificity of Brucella Coombs gel test were found as 100.0 and 82.2%, respectively. Conclusion: Brucella Coombs gel test can be used as a screening test with high sensitivity. By the help of pink Brucella antigen precipitation, the tests’ evaluation is simple and objective. In addition, determination of Brucella antibody by rapid titration offers another important advantage.
  Halil YAZGI , M. Hamidullah UYANIK , Mustafa ERTEK , Ayse Esin AKTAS , Hakan IGAN and Ahmet AYYILDIZ
  Aim: The aim of the present study was to investigate the survival time of some nosocomial infectious agents on the surface of various covering materials.

Materials and Methods: Four kinds of flooring materials (vinyl and ceramic tile as floor covering material; and laminate and inox sheet as bench covering material) were experimentally contaminated with Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and vancomycin-resistant (VR) Enterococcus faecalis, which were frequently encountered in nosocomial infections. The survival periods of representative agents on the tested flooring materials were detected by culturing from the surfaces.

Results: It was found that the survival periods were shorter for all the tested bacteria on the vinyl surfaces, as compared to the ceramic surfaces; the difference was significant (P < 0.05). On the other hand, all the test bacteria survived shorter on the inox sheet than on the laminate surfaces (P < 0.05). On all of the 4 covering materials tested gram-positive bacteria were found to survive longer than the gram-negative ones.

Conclusions: It was concluded that vinyl as flooring material and inox sheet as surface material for benches and laboratory tables may be preferred in order to reduce bacterial colonisation in the hospital environment.

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