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Articles by H.S. Jayasinghearachchi
Total Records ( 5 ) for H.S. Jayasinghearachchi
  H.S. Jayasinghearachchi , G. Seneviratne and H.M.S.P.M. Weerasinghe
  Effects of tannic acid on rhizobial performance and legume-rhizobial N2 fixing symbiosis were examined using soybean as the test plant. Two strains, Bradyrhizobium elkanii SEMIA 5019 and B. japonicum TAL 102 were grown in continuous culture media with different concentrations of tannic acid for 28 days to produce tannic acid-affected rhizobia. Soybean inoculated by the affected rhizobia in gravel pots was evaluated for plant growth, nodulation and N accumulation after 40 days. Two other sets of soybean plants inoculated by unaffected, normal rhizobia were treated with tannic acid separately at planting or two weeks after planting. This was to examine the effect of soil solution tannic acid on the symbiosis. Plant growth, nodulation and N accumulation of soybean were reduced when the rhizobial strains were affected mainly by ≥0.2 mM tannic acid. This was conspicuous in B. japonicum TAL 102. Tannic acid was responsible for biochemical changes of the rhizobial proteins, thus resulting in their alterations, especially in B. japonicum TAL 102. When grown in culture media with 0.4 mM tannic acid, an increased shoot N accumulation was observed in inoculated soybean. It is concluded from this study that soil tannins have both favorable as well as unfavorable effects on the legume-rhizobial N2 fixing symbiosis. They reduce rhizobial performance by direct interactions whereas they enhance N2 fixing activity possibly by removing soil mineral N in the proximity of legume root system at planting.
  H.S. Jayasinghearachchi , G. Seneviratne and H.M.S.P.M. Weerasinghe
  .
  H.S. Jayasinghearachchi , G. Seneviratne and H.M.S.P.M. Weerasinghe
  Present study reports a novel method to fractionate and analyse a mixture of polyphenols without prior purification. It is based on compound separation of the extracts using one dimensional polyacrylamide gel electrophoresis (1D-PAGE) and subsequent determinations of approximate molecular masses and concentrations on the gel. This is a simple and rapid technique, which has important applications in complex interactions of soil-plant systems and also in different areas of plant sciences. These initial results justify the importance of its further improvements, to be used as a cost-effective tool for polyphenol research, compared to conventional analytical methods.
  H.S. Jayasinghearachchi and G. Seneviratne
  .
  H.S. Jayasinghearachchi and G. Seneviratne
  Mycelial colonization and biofilm formation with Pleurotus ostreatus, a mushroom fungus and its effects on the endophytic colonization of tomato by Pseudomonas fluorescens when the plant growth medium was treated with a co-culture of P. fluorescens and P. ostreatus, was investigated aseptically under in vitro conditions. Mycelial colonization by P. fluorescens started one day after co-culturing them in a broth culture. The bacteria colonized heavily on mycelial surfaces of P. ostreatus forming biofilms after 4 days of co-culturing. Endophytic populations of P. fluorescens in leaves, shoots and roots of the plant were higher when the plant growth medium was treated with a biofilmed inoculum than an inoculum with planktonic (freely swimming) bacterial cells (i.e., without the fungus), after 21 days of planting. Plant growth was not affected by P. ostreatus. The results of this study suggest that the formulations of such biofilm based inocula using compatible microbial combinations would be useful for the successful establishment of introduced microorganisms in the plants and the rhizosphere and the enhancement of overall productivity of agricultural ecosystems. Further studies are needed to evaluate these biofilmed inocula under field soil conditions.
 
 
 
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