Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Articles by H.M. Parker
Total Records ( 8 ) for H.M. Parker
  H.M. Parker and C.D. McDaniel
  The Sperm Quality Index (SQI) is capable of predicting semen quality when semen is diluted 10-fold prior to analysis; yet when semen is diluted, changes in motility, gas exchange, Adenosine Triphosphate (ATP) and ion content occur. Therefore, the objectives of this research were to determine if the SQI could be a predictor of ATP, gas and ion content of semen and to determine if O2 and ATP content of semen could be used as predictors of seminal ion content when ejaculates are diluted 10-fold. Prior to semen analysis, ejaculates from 70 Ross males were diluted 10-fold using 0.85% saline and immediately analyzed to determine each ejaculate`s SQI as well as ATP, pH, O2, CO2, Na+, K+, Ca2+ and Cl- content. There was a positive correlation between the SQI and ATP present per sperm (r = 0.70, p<0.0001). However, the coefficients for the SQI with concentrations of O2, Ca2+, Na+ and Cl- present per sperm were negative (r = -0.70, -0.49, -0.51 and -0.53, respectively). The coefficient for the concentration of O2 per sperm with ATP per sperm was negative (r = -0.41) yet the coefficients for CO2, Ca2+, Na+, K+ and Cl- were positive (r = 0.30, 0.74, 0.77, 0.50 and 0.77, respectively). In conclusion, the SQI and O2 present per sperm can predict ATP and ion content in broiler breeder semen samples diluted 10-fold.
  K.J. Barker , J.L. Purswell , J.D. Davis , H.M. Parker , M.T. Kidd , C.D. McDaniel and A.S. Kiess
  A common practice in the commercial broiler industry is to reuse litter over multiple broiler flocks. Over time the bacterial populations in the reused litter increases but how those organisms are spatially distributed throughout the litter bed is unclear. Therefore, the goal of this project was to investigate the distribution of bacteria at three different depths of litter. Litter samples were collected from three commercial broiler houses on three different farms. Four samples from each house were collected using clear PVC pipes which were driven through the litter bed to the clay floor. Each pipe was transported up-right to the lab, where they were cut into three sections (top, middle and bottom) exposing the litter for processing. Litter from each section was serially diluted in peptone and streaked onto either tryptic soy agar or Levin eosin methylene blue agar plates. Plates were incubated under the appropriate atmospheric condition for 24 h at 37oC. After 24 h, plates were counted for total aerobes, anaerobes and coliforms. Results of this study indicate a significant difference (p<0.05) in bacterial counts between the different sections of the litter. The middle and bottom sections had significantly lower anaerobe and coliform counts compared to the bacterial counts in the top sections. In conclusion, the results suggest that the middle and bottom section of litter provide a less favorable environment for bacterial growth than the top section.
  J.B. Wells , C.D. Coufal , H.M. Parker , A.S. Kiess , K.M. Young and C.D. McDaniel
  Research has shown that exterior eggshell Aerobic Plate Counts (APC) are greatly decreased using a combination of Ultraviolet Light (UV) and hydrogen peroxide (H2O2). However, it is unknown how this treatment process would impact hatchability. Therefore, the objective of this experiment was to determine if spraying eggs with 1.5% H2O2 followed by UV irradiation for 8 min to reduce eggshell APC would affect hatchability. Eggs from 3 commercial houses (57 wk-old broiler breeders) were collected over 2 d (n = 1,944 eggs). Half were treated with 1.5% H2O2 and UV and the other half served as untreated controls (18 eggs/ tray). At time of treatment, 1 egg was randomly selected from each of 108 trays (n = 54 per treatment) for eggshell APC enumeration on TSA. Remaining eggs were stored at 18.3oC. Prior to set, 1 egg per tray from d 1 of collection was sampled for APC enumeration. The 16 remaining eggs/tray were weighed prior to incubation and at 18 d to determine egg weight loss. At hatch (21.5 d), chick weights were obtained, meconium samples were collected from 18 chicks per incubator and samples were plated on TSA to determine the presence of intestinal microorganisms. A 3 log10CFU/egg reduction in eggshell APC was found for treated eggs when compared to control eggs. At hatch, no differences in chick weight, egg weight loss, positive meconium samples, or hatchability were observed between treatments. In conclusion, UV irradiation for 8 min with 1.5% H2O2 reduced eggshell APC on broiler breeder eggs with no affect on hatchability.
  J.B. Wells , C.D. Coufal , H.M. Parker , A.S. Kiess , J.L. Purswell , K.M. Young and C.D. McDaniel
  Previous research has indicated that a single exposure of eggs to Ultraviolet Light (UV) in combination with 3% hydrogen peroxide (H2O2) results in a greater reduction of eggshell microorganisms compared to eggs treated with either UV or H2O2 alone. The objective of this study was to determine if hatchability would be affected if eggs were treated by repeated applications of UV and H2O2. In the first experiment, eggs receiving H2O2 and UV light for 2 min 6 times yielded the greatest reduction in aerobic plate counts (5.3 log10CFU/egg) when compared to other treatment groups that utilized various repetitions of H2O2 and UV light. The second experiment determined the effect on hatchability when using this combination tested in Experiment 1. In Experiment 2, a 4 log10CFU/egg reduction in eggshell aerobic plate counts was observed for eggs treated with UV and H2O2 when compared to untreated control eggs. There were no differences in hatchability, hatch residue, chick weight, residual yolk weight, or egg weight loss between control and treated groups. In conclusion, multiple applications of UV and H2O2 effectively reduced aerobic microorganisms on the eggshell to low levels with no detrimental effects on broiler breeder egg hatchability or chick quality parameters.
  K.N. Eberle , J.D. Davis , J.P. Purswell , H.M. Parker , C.D. McDaniel and A.S. Kiess
  In 2009, the USDA Food Safety and Inspection Service announced the development of new pathogen reduction performance standards for Salmonella and Campylobacter both on-farm and in the processing plant. The objective of this study was to evaluate the prevalence and distribution of Campylobacter in 3 newly constructed broiler houses for the first 4 flocks placed. Litter and fecal samples were collected from each house at 0, 28 and 48 d of production. Samples were serially diluted and spread onto Campy Cefex agar plates. Two 40 mL water samples were collected each production day and filtered through a 0.45 μm membrane before being placed onto a Campy Cefex agar plate. All plates were purged with a microaerophilic gas and incubated for 36 h at 42°C. Individual plates were screened for characteristic Campylobacter colonies and suspect colonies were confirmed using a latex agglutination kit. An additional 50 g of litter was collected from the evaporative cooling inlets, middle and tunnel ventilation fans to determine litter moisture and pH. Inside and outside temperatures were also collected. Out of 2300 litter, 900 fecal and 45 water samples, only 5, 6 and 1 of the collected samples, respectively, were confirmed Campylobacter positive. The middle of the house contained a higher litter moisture level (37%) than the evaporative cooling inlet end (33%) and tunnel ventilation fan end (34%) (p<0.05). Litter pH was not different across days, locations or flocks. Temperature averaged 26.8oC inside and 27.6oC outside. In conclusion, the newly constructed houses did not show a high prevalence of Campylobacter. Litter moisture was at levels conducive for Campylobacter growth. The high litter pH and low temperatures, along with other on-farm management strategies and the fact the broiler houses were brand new, may have suppressed Campylobacter’s ability to colonize the litter.
  C.N. Obi , H.M. Parker , A. Corzo and C.D. McDaniel
  Because research revealing the impact of Lys on reproduction in Broiler Breeders (BB) is sparse, this study was conducted to evaluate the impact of digestible Lys (dLys) on BB semen characteristics and BW. Eighty males were caged individually from 20 to 39 wk of age. Treatment 1 and 2 diets had the same level of dLys (1,000 mg/rooster/day) in a corn-soybean meal based diet (Soy 1000) and distillers dried grains with solubles (DDGS; DDGS 1000) diet, respectively. Treatment 3, 4 and 5 diets had the inclusion of DDGS in order to titrate dLys intake levels of 850 (DDGS850), 700 (DDGS700) and 550 (DDGS550) mg/rooster/day, respectively. Body weight and semen samples were determined every 2 wk from 26 to 38 wk of age. Immediately after semen collection, samples were analyzed for semen volume, sperm viability, sperm concentration and the Sperm Quality Index (SQI). BW of roosters fed Soy 1,000 was higher than the other treatments from wk 26 through wk 38. This excess weight could be due to over estimating the energy content of DDGS resulting in diets that were not isocaloric. At 28 wk and continuing through wk 38, the percentage of dead sperm was highest in roosters fed Soy 1000. Also, at wk 38 plasma testosterone concentrations were higher for roosters fed Soy 1000. In conclusion, varying levels of dLys (1,000-550 mg/rooster/day) in a DDGS based diet does not appear to cause adverse effects on BB male semen quality during pre-peak and peak production.
  P. Santa Rosa , H.M. Parker , A.S. Kiess and C.D. McDaniel
  Parthenogenesis, embryos from unfertilized eggs, often exhibit delayed development at oviposition and throughout incubation at standard incubational temperature. Additionally, the first egg in a clutch sequence is more likely to exhibit parthenogenesis than subsequent eggs. Because the first egg in a clutch sequence stays in the hen’s body longer, it is possible that the temperature of the hen’s body accelerates parthenogenetic development. Increasing storage and incubational temperature may simulate the hen’s body temperature and increase the incidence of parthenogenesis. Therefore, the objective of this study was to determine if storage and incubational temperatures impact parthenogenetic development in virgin Chinese Painted quail. Daily eggs were collected, labeled and divided among 3 different storage temperatures (20, 30 or 40°C). Eggs were incubated at 37°C for 10 d or 42°C for 48 h then returned to the standard incubational temperature of 37°C for the remaining 8 d of incubation. After 10 d of incubation, albumen pH and parthenogen size was measured for each egg. At an incubational temperature of 42°C, eggs stored at 20°C yielded the highest percentage of parthenogens. Also, eggs stored at 20°C and incubated at 42°C yielded the lowest albumen pH. As storage temperature increased, parthenogen size increased when eggs were incubated at 37°C. Also when eggs were stored at 30°C, embryo size was larger when incubated at 42°C for 48 h as compared to incubation at 37°C. In conclusion, elevated egg storage or incubational temperatures alter albumen pH, the incidence of parthenogenesis and the size of parthenogenetic embryos.
  M.D. Haines , H.M. Parker , C.D. McDaniel and A.S. Kiess
  Lactobacillus resides in the hen’s gastrointestinal tract, vagina and cloaca. When fed to hens, Lactobacillus has improved egg weight, size and shell strength. However, data from our lab suggests Lactobacillus may be detrimental to semen and possibly fertility. Therefore, the objective of this study was to determine if semen exposed to Lactobacillus affected fertility in hens. For the experiment, semen was collected from 40 roosters and used to create 4 treatments: saline, broth, 103 cfu/mL of Lactobacillus and 106 cfu/mL of Lactobacillus. Each treatment was diluted 1:1 with pooled semen and sperm quality index readings were obtained prior to insemination. Eighty hens in each treatment were equally divided among 10 blocks and inseminated. Eggs were collected from 2 to 7 d post-insemination (DPI) and hens were artificial insemination again on DPI 8, eggs were collected for another 7 d. Eggs were incubated for 10 d, candled for fertility and a portion of the eggs were examined for Lactobacillus. The entire experiment was repeated twice. Semen quality was within the normal range for treatments 1, 2 and 3, but semen from treatment 4 was immotile before insemination. Additionally, 84% of the eggs from treatments 1, 2 and 3 were fertile; however, all eggs from treatment 4 were infertile. There was no difference between treatments for Lactobacillus counts in the yolk or on the shell. However, fertility and Lactobacillus shell counts declined over DPI. In conclusion, if Lactobacillus exceeds 106 cfu/mL in the hen or roosters reproductive tract, fertility could be impacted.
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility