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Articles by H. Tajik
Total Records ( 3 ) for H. Tajik
  F. S. Sabet Jalali , A. Ehsani , H. Tajik and S. Ashtari
  Unprocessed honey is well recognized as wound-healing remedy. However, to make use of the honey clinically acceptable, it should be sterilized. It is well-established that the antibacterial activity is heat-labile either by using sterilization by autoclaving, but the effectiveness of gamma-irradiation on the antibacterial activity of honey is unknown. Therefore, an investigation was carried out to assess the effects of the antibacterial activity of Iranian honey using the commercial gamma-irradiation sterilization procedure. The honeys were divided into 4 groups (0, 5, 15 and 25 KGy). Then each group was divided four sub-groups non-irradiated and irradiated (non-heated, 25C, 35C, 45C). Microbiological test of the honeys were carried out against control organisms (Staphylococcus aureus , Escherichia coli and Pseudomonas aeruginosa) and clinically isolated organisms were tested in an agar disk diffusion assay. Results showed that there were no significant changes using the antibacterial activity of gamma-irradiation sterilization of honey, even the radiation was 25 KGy. The results of the study indicate that a better antibacterial activity is derived using the gamma-irradiation technique to maintain sterility and produced undesirable effect on antibacterial activity of honey.
  S.S. Saei Dehkordi , S.M. Razavi Rohani , H. Tajik , M. Moradi and J. Aliakbarlou
  The antibacterial efficacy of unheated lysozyme (L) and Heat-Treated Lysozyme (HTL) at 62, 72 and 80C, separately and in combination with Zataria multiflora Boiss. essential oil (Z.EO) on E. coli O157:H7 and S. aureus at different pH (5, 6 and 7) and NaCl concentrations (0.5, 1.5 and 3%) was studied. A micro-broth dilution assay was applied to define the Minimal Inhibitory Concentrations (MICs). The inhibitory action of L on S. aureus was increased along with pH deduction and NaCl enhancement, whereas E. coli O157:H7 wasnt inhibited up to 1024 g mL 1 at similar circumstances. Both bacteria were inhibited by HTL (at 72 and 80C) at NaCl 0.5%, but at 1.5% of NaCl concentration, the inhibition was stopped. However, the inhibitory activity of HTL was greater than L. Z.EO was effective against both bacteria and inhibition was stronger at lower pH and higher NaCl concentrations. MICs of L and HTL combined with Z.EO were decreased. Enhancement of Z.EO concentrations was led to more prominent inhibition, whereas, by increasing NaCl concentration, inhibition was reduced considerably. The lowest MICs of L and HTL were achieved by increase of Z.EO to 300 g mL 1 along with decrease the pH and NaCl to 5 and 0.5%, respectively.
  H. Tajik and F.S.S. Jalali
  Achillea millefolium, belonging to the Asteraceae family, is used widely in many parts of the world. There are many reports about therapeutic application of yarrow in treatment of various diseases. But the documented information about the enhancing effect of yarrow extract on healing of the burn wound is few. The aim of this investigation was a clinical and microbiological evaluation of the accelerating effect of the yarrow (aqueous extract) on the burn wound healing in rabbit. A total of 10 male white rabbits, with mean weight of 2,000250 g were studied. Based on Hoekstra standard model, burn wounds (2025 mm2) were created in dorsal region of each animal. Five mililiter of aqueous extract of yarrow used on the experiment wounds every day for 21 days. In the control group, the wounds were washed with normal saline at same frequency and time of day. Clinical and microbiological examinations of the burn wounds were carried out on 0, 7, 14 and 21days of the experiment. The wounds were photographed and compared for rate of wound contraction with digital scanning software. Wounds treated with yarrow extract had more improved healing appearance and the rate of contraction in contrast to the control wounds. Furthermore, a lesser total count of microorganisms was found (20.4 104 cm-2) when yarrow extract was applied on burn wounds compared to the control wounds (10.5105 cm-2) on the day 21 (p<0.05). According to the results of this study, topical application of the aqueous extract of Achillea millefolium could enhance burn wound healing process in the rabbit from clinical and microbiological aspects.
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