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Articles by Guo-Yue Chen
Total Records ( 4 ) for Guo-Yue Chen
  Ping-ping Zhuang , Qin-ce Ren , Wei Li and Guo-Yue Chen
  To estimate the allelic variation at the expressed sequence tag simple sequence repeats (EST-SSR) among Persian wheat (Triticum turgidum ssp. carthlicum L.) accessions collected from fifteen countries and provide information for wheat breeding and improvement in southwest China, 87 Persian wheat accessions from fifteen countries were investigated by using EST-SSR markers. EST-SSRs were molecular markers belonging to the transcribed region of the genome. Therefore, any polymorphism detected by EST-SSRs might reflect better relationship among species or varieties. Fourteen primer pairs could successfully amplify the fragments in the 87 accessions, of which tri-nucleotide repeats were the dominant type. A total of 33 eSSR alleles were detected, and the number of alleles detected by single pair primers ranged from 3 to 7 per locus, with an average of 3.71. Clustering analysis suggested that most of the accessions with adjacent geographic origins had the tendency to cluster together. Therefore, when used in Persian wheat genetic analysis, EST-SSR markers not only act as genetic markers but also reveal differences in related gene expression.
  Li-Juan Yang , Ya-Xi Liu , Bi-Ling Xu , Wei Li and Guo-Yue Chen
  One hundred and thirteen low-molecular-weight glutenin subunits encoding sequences and parts of upstream were characterized from Triticum dicoccoides. The encoded proteins of 113 genes had similar structures to previously characterized LMW-GS. These sequences had 856~1402 nucleotides in length with 2~26 repeat motifs. Most of the sequences were typical LMW-m glutenin subunits genes and the frequency of SNPs was 1.6 out of 10 bases and A-G mutation was the most frequent. Fourteen deduced amino acid sequences were found to be possessed with an additional cysteine residue in C-ter I. Thirty-six haplotypes were detected and phylogenetic analysis indicated that the 36 haplotypes could be classified into 3 haplotype groups. Individually classifications based on the four main domains of LMW-GS DNA sequences, 5’flanking, single peptide, N-terminals and C-terminal, were in agreement with the classification based on the coding regions. Consilient evolution was found between domains of LMW-GS as well as each domain and the whole coding region. The results revealed the important information of low-molecular-weight glutenin subunit gene family and contributed to our understanding of functional aspects of the low-molecular-weight glutenin subunit genes.
  Ya-Xi Liu , Wei Li , Yu-Ming Wei , Guo-Yue Chen and You-Liang Zheng
  This study characterizes 15 waxy genes from 15 accessions of the einkorn wheats Triticum urartu, T. boeoticum and T. monococcum. The mature protein coding sequences of waxy genes were analyzed. Nucleotide sequence variations in these regions resulted from base substitution and/or indel mutations. This work identified 8 distinct haplotypes from the diploid wheat waxy gene sequences. A main haplotype was found in 7 gene samples from the Au genome and Am genome. The waxy gene sequences from the Au and Am genomes could be obviously clustered into two clades, but the sequences from the Am genome of T. boeoticum and T. monococcum could not be clearly distinguished. The phylogenetic analysis revealed that the waxy gene sequences from the Am genome had accumulated fewer variations and evolved at a slower rate than the sequences from the Au genome. These results would contribute to the understanding of functional aspects and efficient utilization of waxy genes.
  Ya-Xi Liu , Xiao-Huan Sun , Bi-Ling Xu and Guo-Yue Chen
  Plant architecture is governed by the action of meristems. During vegetative development, the shoot apical meristem is responsible for initiating all of the above-ground structures including the nodes, internodes, leaves, axillary meristems and the inflorescence. Five barley mutants with low-tiller have been found, currently including, low number of tillers1 (lnt1.a), absent lower laterals1 (als1), intermedium-b (int-b), uniculm2 (cul2.b), uniculm4 (cul4) and semi brachytic (uzu). Specifically, the cul2.b mutant failed to develop tillers, while the lnt1.a mutant can produce 1-4 tillers. Genetic analysis indicated that two mutant phenotypes were caused by two recessive genes cul2.b and lnt1.a, respectively. In this study, two F2 populations, 279 individuals derived from BowmanxGSHO 531 and 184 individuals derived from BowmanxGSHO 1984 were developed for mapping the cul2.b and lnt1.a genes using Simple Sequence Repeats (SSR) markers. F3 populations were created to identify genotypes of F2 individuals. Ultimately, cul2.b was located between SSR markers GBM1212 and Bmag 0613 on the long arm of chromosome 6H, with distances of 12.7 and 13.2 cm to the two markers, respectively. Another five SSR markers (GBM 1319, GBM 1423, Bmag 0807, Bmag 0378 and Bmag 0003) on chromosome 6H were also found around the cul2 gene, with distances of 19.6, 33.3, 34.1, 71.5 and 80 cm to the cul2.b gene. The lnt1.a gene was positioned 7.8 cm away from GBM 1043 on chromosome 3H. This study narrowed the block of tiller development gene in the cul2 and lnt1 mutant. It is a benefit for further map-based clone of the genes.
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