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Articles by G.P. Dubey
Total Records ( 4 ) for G.P. Dubey
  G.C. Minakshi , K. Vasanth , Tanupriya , K. Ilango , Aruna Agrawal and G.P. Dubey
  Diseases caused due to ‘oxidative stress’ initiated by Reactive Oxygen Species (ROS) is growing at an alarming rate. Several medicinal herbs explored till date were reported to possess numerous pharmacological properties used for combating different diseases. The present study was undertaken to investigate the inhibitory potential of Terminalia chebula by H2O2 induced oxidative stress in THP-1 cell line and also the presence of the marker compounds in T. chebula was confirmed by High Performance Liquid Chromatography (HPLC). Intracellular quantification of ROS was done using a flow cytometer. The percentage of gallic acid and ellagic acid in the extract was performed by HPLC were found to be 4.84% w/w and 2.56% w/w, respectively. Pretreatment of cells with T. chebula extract significantly reduced the intracellular ROS production in a dose-dependent manner. The present study illustrates the effective role of T. chebula in reducing the ROS production which is a causative factor for several disorders. The results describe the possibility and potentiality of natural sources to inhibit the pathogenesis of different diseases caused due to oxidative stress.
  G.C. Minakshi , K. Vasanth , Tanupriya , K. Ilango , R. Mohan Kumar , Aruna Agrawal and G.P. Dubey
  Garcinia indica have been used for centuries with claim for its medicinal value by traditional practitioners to treat numerous diseases. The anti-oxidant activity of G. indica fruit which were assessed by determining the total phenolic content, evaluating the 2, 2-diphenyl-1-picrylhydrazyl, (DPPH) activity, superoxide dismutase (SOD) activity, as well as its ability to inhibit Nitric Oxide (NO) and also their effect on H2O2 induced Reactive Oxygen Species (ROS) generation in human monocytic (THP-1) cells was investigated by tracking intensity of a cell permeable fluorescent dye. In addition, High Performance Liquid Chromatography (HPLC) was used to quantify the bioactive constituent of G. indica fruit extract responsible for its biological activity. The fruit extract of G. indica had the highest amount of hydroxycitric acid (HCA) and phenolic content showed potent antioxidant activity. The IC50 values for DPPH, SOD and NO scavenging activities were 50.34, 37.08 and 34.15 μg mL–1, respectively. Garcinia indica fruit extract (200 μg mL–1) attenuated ≥60% of H2O2 mediated ROS generation in THP-1 cells. The above data provides evidence that the fruit extract of G. indica is rich in natural anti-oxidants and thus justify its use in folk medicine especially in the management of free radical-mediated disorders.
  I. Jyothi , Tanu Priya , T.M. Vijayakumar , S. Ramesh Kannan , K. Ilango , A. Agrawal and G.P. Dubey
  Use of Clonazepam was very common in Parkinson’s patient with sleep problems such as rapid eye movement behavior Disorder. A pilot study was performed to evaluate the effects of Clonazepam on sleep pattern in Parkinson’s patient using video polysomnography. Overnight polysomnography was performed prospectively in three healthy males (age 51.33±3.51 years) and with three male Parkinson’s patient (age 60.00±8.00 years) with sleep disorders. The study was conducted in two phases using standard techniques in accordance with guidelines published by the American Academy of Sleep Medicine (AASM). For sleep stages evaluation, an electroencephalogram (4 channels with 2 central and 2 occipital) chin electromyogram (with 1 channel) and electro-oculogram (with 2 channels) were achieved. Polysomnography characteristics of healthy volunteers and Parkinson’s patient with and with out Clonazepam effect was evaluated. Sleep latency was compared between healthy volunteers and Parkinson’s patients in both the phases. The difference was highly significant [Phase I (p = 0.004, R2 = 0.896), Phase II (p<0.001, R2=0.999)], but there was no considerable effect of Clonazepam in PD Patients [Phase I, (p = 0.606, R2=0.072) Phase II (p = 0.726, R2 = 0.081)]. Clonazepam significantly increases the sleep efficiency [Phase I (p<0.001, R2 = 0.991) Phase II (p = 0.002, R2=0.998)] in Parkinson’s patients but Clonazepam did not have any significant effect on wake after sleep onset, stage I sleep, sleep latency and wakefulness. Hence a larger population based longitudinal study should be performed to validate these findings.
  K. Ilango , N. Kasthuri Bai , R. Mohan Kumar , K. Ananth Kumar , G.P. Dubey and Aruna Agrawal
  High altitude herbal medicines offers remedy for many diseases, particularly for which no medicine is available. As high altitude plants grow under stressful situations and exposed to high UV radiations, they are reported to have immense potential. Various scientific studies conducted on Hippophae species (Elaeagnaceae) during the last decade confirm its medicinal values. The present study was carried out with a view to lay down standards which could be useful to detect the authenticity of these medicinal plants, further the microscopical and physiochemical evaluation were studied to reveal the differences among Hippophae rhamnoides L. and Hippophae salicifolia D. Don. For the microscopic observation, free hand cross sections of the leaves of Hippophae rhamnoides and Hippophae salicifolia were stained with phloroglucinol and hydrochloric acid (1:1) and studied according to standard methods. Physiochemical analysis was carried out as per WHO guidelines on quality control methods for medicinal plants. The cross section of both the species of leaves were almost similar and revealed that the lower epidermal cells were completely covered with apressed stellate trichomes and abundant sunken stomata. Ash values of Hippophae rhamnoides leaves, showed higher ash content, compared to Hippophae salicifolia. The Pharmacognostic and phytochemical profile of Hippophae rhamnoides and Hippophae salicifolia are highly dependent on environmental adaptability of the plants. The present study helps in identification and differentiation of both the species of Hippophae.
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