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Articles by G.H. Zhang
Total Records ( 3 ) for G.H. Zhang
  X.D. Hu , K. Jia , G.H. Zhang , Z.X. Lin , H.Y. Xia , D.W. Zhang , X.B. Zeng , H.S. Qiao , H. Zhang and S.J. Li
  Several Enzyme-Linked Immunosorbent Assay (ELISA) methods exist to diagnose Bovine Tuberculosis (BTB). In this study, an ELISA method was established with a mixture of esat-6 and cfp-10 as the diagnostic antigens. ELISA tests on 160 bovine serum samples (80 from cattle with BTB; 80 from healthy cattle) demonstrated that the sensitivity was 76.3% (61/80) and the specificity was 97.5% (78/80). A further screening test using ELISA and PPD was conducted with 335 serum samples collected at random from eight dairy herds (5 problematic herds and 3 BTB-negative herds) in Guangdong, China. In the problematic herds, the positive ELISA rate was 7.9% (16/202) for anti-esat-6 and cfp-10 antibodies and 5.4% (11/202) in the PPD. This study demonstrated that ELISA with the use of the esat-6 and cfp-10 mixture of antigens is simple and sensitive and can be used to analyze large numbers of samples for the serodiagnosis of BTB.
  M. Yu , Z.Y. Ning , Z.X. Cao , R.S. Li , C.L. Huang and G.H. Zhang
  In the present study, researchers describe a rescue system for duck hepatitis virus-1 using T7 RNA polymerase. This strain can not induce typical cytopathic effect in BHK-21 cells. First, there is used the vitro transcripts to infect BHK-21 cells. Then the infected cell supernatant were inoculated into chicken embryos to rescue the virus and the cDNA clone was engineered to contain one silent nucleotide change to create a BssHII site to distinguish from the parental virus. The rescued virus was able to result in embryonic death and have similar infection with parental virus in chicken embryos indicating that the cDNA clones were replication competent. It is proposed that this rescue system may benefit the investigation on viral virulence determinants and molecular virology of DHV-1 virus.
  Z.H. Sun , G.H. Zhang , X.Q. Wang , G. Yu , L.Y. Zhu , H.L. Fan and D. Xu
  The organic nonlinear optical crystal of amino-carboxyl acid family, l-lysinium trifluoroacetate (abbreviated as LLTF) was successfully grown from its aqueous solution by the temperature-lowering technique. Its growth morphology was investigated based on the X-ray single-crystal diffraction data and the growth habits were studied using the micro-crystallization method. The metastable zonewidth and induction period were determined for the aqueous solution growth of LLTF. The surface free energy values were calculated from the experimental data. Nucleation kinetics and fundamental growth parameters such as critical radius, critical free energy and volume free energy change were also evaluated. Crystals of LLTF were obtained by the optimized nucleation parameters.
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