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Articles by G. Singh
Total Records ( 2 ) for G. Singh
  S.R. Mishra , Jaya Bharati , M.K. Bharti , G. Singh and M. Sarkar
  Fibroblast Growth Factor (FGF) family is considered as local cytokines produced from many physiological systems including ovary. The FGFs regulate many cellular processes like angiogenesis, cell proliferation, cell survival, organogenesis and embryonic development including ovarian folliculogenesis. Fibroblast Growth Factor 10 (FGF10) is one of most important member in FGF family which mostly mediates epithelial cell motility, differentiation, migration and wound healing in many cellular system. The FGF10 is also known to regulate the ovarian follicular dynamics in an autocrine and paracrine manner. Therefore, the present study was undertaken aimed with an objective to evaluate the expression and localization of FGF10 in different follicular size or groups during various stages of follicular development in buffalo ovary. The mRNA and protein expression of FGF10 transcripts did not differ significantly in all follicular size during follicular development. The FGF10 was exclusively localized in Granulosa Cell (GC) and Theca Interna (TI) showing a stage specific immunoreactivity throughout the follicular developmental process in buffalo ovary. The present findings suggest the species specific expression and localization pattern of FGF10 in buffalo ovarian follicle.
  S. Sharma , A.P. Garg and G. Singh
  The present study was carried out to optimized medium composition and culture conditions to enhance the bacteriocin production from Lactococcus lactis CCSULAC1 in MRS medium. The optimum temperature and initial pH for bacteriocin production was 7.5 and 35°C, respectively. Lactococcus lactis CCSULAC1 displayed the highest bacteriocin activity when grown in modified MRS medium containing soya extract (SEMRS) as nitrogen source instead of other selective constituents. Different culture media including MRS with 1.5% tween, TGEA, TGYA, Elliker’s media were also studied for bacteriocin production. The bacteriocin showed broad antimicrobial spectrum determined by well diffusion assay against E. coli, Enterobacter sp., S. aureus, Pseudomonas sp., B. polymyxa, B. subtilis, S. typhii, Micrococcus sp. but shown no effect on Candida albicans, Shigella sp., Klebsiella sp. and S. paratyphii. Replacement of soya extract with yeast extract demonstrated the better yield of bacteriocin. The modified SEMRS is a cost effective medium that can be helpful for large scale industries.
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