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by
G. Mosayebi |
Total Records (
6 ) for
G. Mosayebi |
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A. Ghazavi
,
G. Mosayebi
,
E. Mashhadi
,
M.A. Shariat-Zadeh
and
M. Rafiei
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This study was planned to determine the levels of uric
acid (UA) and CRP at preeclampsia and their association with the severity
of the disease. In a cross-sectional, case-control study we measured UA
and CRP levels in blood samples from 46 women with preeclampsia, 23 normal
pregnant women and 23 non pregnant women matched for age, BMI, parity
and gestational age were measured. Twenty three patients had developed
severe and 23 mild preeclampsia. UA and CRP were measured by enzymatic
method and enzyme-linked immunosorbent assay, respectively. Roc curve
was used to determine the optimal cutoff value. Results showed CRP and
UA concentrations were higher in Preeclamptic group (33.77 ±25.97,
5.93 ±0.75) compared with normal pregnant group (17.31 ±19.54,
5.47 ±0.41). CRP levels were also significantly elevated in women
with severe preeclampsia compared to those mild preeclampsia (42.26 ±24.04,
16.81 ±22.03). Determination of serum CRP levels may be used as
marker for the severity of preeclampsia. We also suggest that serum UA
level of 5.5 mg dL-1 is best cutoff point for the diagnosis
of preeclampsia. |
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H. Abtahi
,
A. Ghazavi
,
M. Karimi
,
S. Mollaghasemi
and
G. Mosayebi
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The aim of this study was to test the antimicrobial activity of the methanolic
extract of bitter apricot seeds. Bitter apricot seeds used in folk medicine
in the treatment of skin diseases and parasitic diseases. It been traditionally
used to treat parasitic infections and skin diseases. Water and methanol
extracts of bitter apricot seeds were screened against some bacterial
strains. Seeds were extracted by percolation method. Aliquots of the extracts
at variable concentrations were then incubated with different bacterial
strains and the antimicrobial activities of the water and methanolic extracts
from bitter apricot seeds were determined by MIC. Three antibiotics were
used as reference compounds for antibacterial activities. Bitter apricot
seeds extract inhibited significantly the growth of the tested bacterial
strains. Among the bacterial strains tested, Staphylococcus aureus
was most susceptibility. The highest antibacterial was exhibited by water
extract. Results from these findings suggest that this bitter apricot
seeds extract may be used as natural antibacterial for treatment of some
of diseases, especially local skin diseases. |
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G. Mosayebi
,
A. Ghazavi
,
H. Salehi
,
M.A. Payani
and
M.R. Khazaei
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Sesame oil was evaluated in the treatment of in C57BL/6 mice. It has profound anti-inflammatory activity and been traditionally used to treat inflammatory disorders. EAE was induced by immunization of 6-8 week old mice with MOG35-55 with complete Freunds adjuvant. Therapy with sesame oil was started on day 3 before the immunization. Total Antioxidant Capacity (TAC) was assessed by Ferric Reducing-antioxidant Power (FRAP) method. Nitric Oxide (NO) production was also estimated by Griess reaction. For histological analysis, mice brain was harvested and sections were stained with Hematoxylin-Eosin. After daily intraperitoneal dosage the sesame oil significantly reduced the clinical symptoms in C57BL/6 mice with EAE. Also, treated mice displayed a significantly delayed disease onset compared with control mice. Sesame oil significantly increased TAC, but it’s effect on serum nitrite production was not significant. Typical brain leukocyte infiltration was observed in control mice compared with treated mice. Present results suggest for the first time that sesame oil therapy may be effective in the prevention of symptomatic EAE. This resistance to encephalomyelitis may be associated with inhibition of oxidative stress. |
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H. Abtahi
,
A.H. Salmanian
,
S. Rafati
,
G.B. Nejad
,
M. Saffari
,
A. Ghazavi
and
G. Mosayebi
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This study was evaluated the ability of DNA vaccine
encoding L7/L12 protein of Brucella sp. to induce cellular and
humoral immune responses in BALB/c mice and the profile of cytokines
and IgG sub classes were determined. Intra muscular vaccination of mice
using L7/L12 gene. Three vaccinations at 3 week intervals were performed.
Cytokines and IgG subclasses were analyzed 3 week after the last DNA vaccination.
Splenic lymphocytes from L7/L12pCDNA3-vaccinated mice produced high levels
of IFNγ (3100 pg mL-1) and low levels of IL-5 (300 pg
mL-1), 3 weeks post-vaccination. The L7/L12pCDNA3 immunizations
elicited high IgG2a isotype response in mice immunized. This antigen also
induced IgG1 titers which were slightly lower than the IgG2a titers. Immunological
analysis shows the appropriate immune response in BALB/c mice model
after vaccination with L7/L12 gene. The high level of IFNγ and low
level of IL-5 in combination with high IgG2a/ IgG1 ratio show the activation
of Th1 cell response. The lower bacterial cfu from vaccinated mice in
comparison with control groups show the efficiency of L7/L12 DNA vaccination
in mice model. |
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A. Ghazavi
,
G. Mosayebi
,
H. Salehi
and
H. Abtahi
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In this study, effect of ethanol extract of Saffron (Crocus sativus L.) in the treatment of Experimental Autoimmune Encephalomyelitis (EAE) in C57BL/6 mice was evaluated. EAE was induced by immunization of 8 week old mice with MOG35-55 with complete Freunds adjuvant. Therapy with saffron was started on day the immunization. Total Antioxidant Capacity (TAC) was assessed by Ferric Reducing-Antioxidant Power (FRAP) method. Nitric oxide (NO) production was also estimated by Griess reaction. For histological analysis, mice brain was harvested and sections were stained with Hematoxylin-Eosin. After daily oral dosage the saffron significantly reduced the clinical symptoms in C57BL/6 mice with EAE. Also, treated mice displayed a delayed disease onset compared with control mice. TAC production was significantly elevated in saffron treated mice. Effect of saffron on serum NO production was not significant. Typical spinal cord leukocyte infiltration was observed in control mice compared with saffron treated mice. These results suggest for the first time that saffron is effective in the prevention of symptomatic EAE by inhibition of oxidative stress and leukocyte infiltration to CNS and may be potentially useful for the treatment of Multiple Sclerosis (MS). |
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S. Mahmoudi
,
H. Abtahi
,
A. Bahador
,
G. Mosayebi
and
A.H. Salmanian
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Streptokinase (SK) is a potent plasminogen activator with widespread clinical use as a thrombolytic agent. In this study, we produce high level expression of recombinant streptokinase in E. coli by expression vector pET32a. Genomic DNA of streptokinase gene (SKC) was extracted, then amplified by polymerase chain reaction (PCR) method and sub-cloned to prokaryotic expression vector pET32a. Escherichia coli BL21 (DE3) pLysS were transformed with pET32a-skc and gene expression was induced by IPTG. The expressed protein was purified by affinity chromatography by Ni-NTA resin. High concentration of the recombinant protein obtained from the single-step purification by affinity-chromatography (Ni-NTA). The yield of recombinant streptokinase was nearly 470 mg L-1 of initial culture. Our data showed that production of recombinant streptokinase improved by pET32a in Escherichia coli. |
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