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Articles by F.J.C. Odibo
Total Records ( 3 ) for F.J.C. Odibo
  U.O. George-Okafor and F.J.C. Odibo
  There are limited reliable information on the commercial production and utilization of proteases for detergent and other industrial uses in Nigeria. Hence, the purification and characterization of Bacillus sp. Gs-3 protease for its potential industrial uses were investigated. The dialyzed crude enzyme was purified 17-fold in a two-step procedure involving carboxymethyl sepharose ionic-exchange chromatography and phenyl sepharose 6-fast flow hydrophobic interaction chromatography. The purified enzyme had its optimal activity at pH 9.0 and 90°C and was stable over a pH range 8.0-11.0. It readily hydrolyzed all the tested protein substrates but exhibited highest affinity for gelatin (Km 0.15 mg mL-1). It retained at least 66.2±0.02% of its original activity in the presence of the tested local commercial detergents and removed bloodstains completely. Its activity was significantly (p>0.05) enhanced by Cu2+ ion but strongly inhibited (75.6±0.07%) by Phenyl-methyl Sulfonyl Fluoride (PMSF). Thus, the enzyme demonstrated desirable properties suitable for its biotechnological applications especially in detergent industry.
  L.O. Eneje , F.J.C. Odibo and C.D. Nwani
  Two millet varieties (P. maiwa and Sossat) of similar nitrogen contents were malted for 6 days under similar conditions to investigate the pattern of diastatic power and hot water extract development of their endosperm materials. Both parameters were found to increase as germination lasted up to the 5th day of germination, supporting high amylolytic activity of starch degradation of enzymes. A very strong correlation analysis value of 0.9444 was found between the two varieties over the same germination time. In like manner when correlation analysis for the development of hot water extract was performed for both samples over the same period, a strong correlation value of 0.9287 was obtained. Both varieties have demonstrated high potential of producing enzymes during malting making them very good candidates for producing high quality malts for beverage production.
  E.U. Opara and F.J.C. Odibo
  Investigation was conducted in the laboratory to characterize, identify and determine the strains of bacteria responsible for bacterial spot disease of tomato in humid tropics of South Eastern Nigeria. The bacteria were isolated from infected tomato plant; leaf, fruit and seed and subjected to some standard bacteriological tests. The results of the cultural, physiological and biochemical analyses obtained showed that the bacteria isolated from bacterial spot lesions of tomato fruits, leaves and seed were gram negative, yellow aerobic rod shaped bacteria with a polar flagella. The bacteria colonies exhibited strong starch hydrolysis, metabolized glucose and produced acid from arabinose, sucrose and cellobiose but not from ducitol or sorbitol. Also nitrite was not reduced to nitrite based on bacteriological characteristics, the bacteria strains were identified as Xanthomonas campestris pv. vesicatoria (ex Doidge). The pathogens were found to be influenced by temperature with optimum temperature of 30-32°C but little or no growth at 20 and 45°C.
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