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Articles by F.A.M. Khalil
Total Records ( 2 ) for F.A.M. Khalil
  M.A. Hassanain , E.H. Abdel Rahman and F.A.M. Khalil
  The in vitro activity of three plant extracts; Artemisia cina, Peganum hormala and Calendula micrantha was investigated against Ascaridia galli adult worms. In vitro treatment of A. galli worms with different concentrations of each extract revealed potency of C. micrantha over the other two extracts as judged by the calculated LD50 values. These values were 2.66 ppm for C. micrantha, 33.7 ppm for P. harmala and 48.98 ppm for A. cina. The impact of the most potent extract, C. micrantha on A. galli worms was detected by scanning electron microscopy. C. micrantha treated worms showed enlargement and destruction of lips with subsequent damage of buccal cavity organelles. Moreover, papillae were disturbed and the amphids structures were lost. The cuticle showed wrinkled surface with loss of striations. Also, the cloacal structures were affected.
  H.A. Shalaby , F.M. El-Moghazy and F.A.M. Khalil
  In this study, specificity of snail feet and hepatopancreases antigens in antibody detection of their trematode parasite was evaluated via Western blot technique. Snails, of two different families, antigens; Lymnaea cailliaudi as intermediate host of Fasciola gigantica and Biomphalaria alexandrina as intermediate host of Paramphistomum microbothrium, were evaluated in detection of IgG antibodies against their trematode parasites after preparation of the required Hyper-Immune Sera (HIS) in rabbits. The results revealed higher specificity of snail feet in antibody detection than hepatopancreases antigens. Where, three of sex polypeptides of L. cailliaudi feet antigen; identified by F. gigantica HIS, showed specific positive reactivity. These polypeptides were at molecular weights of 59, 57 and 52 kDa. While, one of sex polypeptides of L. cailliaudi hepatopancreases antigen; identified by F. gigantica HIS, at molecular weight of 57 kDa was specific. Similarly, two polypeptides of B. alexandrina feet antigen; at molecular weights of 54 and 45 kDa, showed specific reactivity toward anti-paramphistomum antibodies. At a time, no specific reactivity had been shown by the antigenically active polypeptides of B. alexandrina hepatopancreases antigen. Owing to its cross-reactivity with incompatible trematode parasite, snail antigen can be used as a general starting antigen for immunodiagnosis of trematode infections.
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