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Articles by F.A. Akinyosoye
Total Records ( 5 ) for F.A. Akinyosoye
  M.K. Oladunmoye , F.C. Adetuyi and F.A. Akinyosoye
  The effect of ethanolic leaf extract from Cassia hirsuta (L.) on the DNA profile of some selected pathogenic microorganisms were investigated using PCR-RAPD analysis to generate DNA fingerprints. The change in molecular configuration of organisms with and without extract shows a wide disparity between the sensitive and the resistant organisms. Thus, this study demonstrates that some of the organisms are susceptible to the antimicrobial activity of Cassia hirsuta extract while some are resistant. The mechanism of action of the extract or other antimicrobial agent on these organisms is due to alteration on the genome and the extract is mutagenic on the susceptible organisms.
  F.O. Omoya , B.E. Boboye and F.A. Akinyosoye
  The type of bacteria present on two insects (cockroach and mosquito) were investigated. These microorganisms were screened for microbial control of mosquito employing their degradative ability at various microbial cell loads. The degradation of the mosquito was observed spectrophotometrically for an incubation period of 5 to 7 days. Six bacterial species (Escherichia coli, Pseudomonas aeruginosa, Bacillus cereus, Serratia marcescens, Staphylococcus aureus and Aerobacter aerogenes) were isolated from cockroach. Mosquito borne E. coli, P. aeruginosa, B. cereus and Staph. aureus. All the bacteria digested the mosquito with activity ranging from 0.02 to 1.27. Microorganisms associated with cockroach showed higher degradation activity (0.02-1.27) during the incubation than those obtained from mosquito (0.02-1.00).
  D.V. Adegunloye , F.C. Adetuyi , F.A. Akinyosoye and M.O. Doyeni
  Microbial analysis of compost using cow dung as booster was investigated. Compost supported high population levels of bacteria with 86% of cultures tested being gram-positive and negative. The microbial identification system based on biochemical analysis identified different groups of bacteria. The microorganisms isolated and identified were Bacillus pumilus, B macereans, B. sphearicus B laterosporus, Micrococcus varians, Proteus mirabilis, Enterobacter aerogenes for bacteria, while Aspergillus rapens, A niger, A. flavus, A. fumigatus, Mucor mucedo, Rhizopus stolonifer, Varicosporium and Fusarium, were isolated for fungi. The microbial loads of the compost varies between 1.6 x 106 and 1.2 x 107 cfu/ml for bacteria, 5.0 x 104 and 5.0 x 107 cfu/ml.
  M.K. Oladunmoye , F.C. Adetuyi and F.A. Akinyosoye
  The rate of release of sodium and potassium ions by aqueous and ethanolic extract of leaves of Cassia occidentalis was investigated for some selected pathogenic bacteria in the genera Bacillus, Staphylococcus, Echerichia, Streptococcus, Klebsiella, Pseudomonas and Salmonella using flame photometer. The aqueous extract was found to be more effective in the leakage of Na+ and K+ ions than the ethanolic extract for all organisms investigated except Salmonella. The aqueous extract released 2.66 ppm sodium ions on Pseudomonas aeruginosa, whereas ethanolic extract released 13.3 ppm while the K+ ions released are 9.282 and 49.980 ppm for ethanolic and aqueous extract, respectively. It was found that only Salmonella typhii gives higher leakages of sodium and potassium ions for ethanolic extract than the aqueous extract. Comparison of the amount of Na + and K+ ions release by the plant extract with two commercial antibiotic (Chloramphenicol and tetracycline) showed that the latter gives a higher value than the former. For sodium ion, Bacillus substilis gives 167 ppm and 164 ppm for chloramphenicol and tetracycline respectively where as 2.28 and 3.42 ppm was measured for ethanorlic and aqeous extract of the Cassia occidentalis responsively. There was no significant difference in the amount of leaked Na+ ions and potassium ions between the two antibiotics. For Na+, Salmonella typhi has 164 ppm for chloramphenicol and 163 ppm for tetracycline while the value for Proteus vulgaris was 160 and 163 ppm, respectively.
  R.A.O. Gabriel , F.A. Akinyosoye and F.C. Adetuyi
  In order to investigate the role of the individual mould strains in the improvement of the nutritional status of jack beans during natural fermentation. The part played by individual mould strains was investigated using each as a starter culture inoculum, using pressure-cooking as the mode of thermal treatment. One hundred gramme of ground, 40 min, pressure cooked samples were inoculated with the single starter cultures under aseptic condition using 1 mL of each inoculum and the samples were homogenized using a sterile scapula after which fermentation was allowed to take place under controlled environment of temperature of 30±3°C for 14 days. Physical sensory changes, fermentation parameters, proximate and antinutritional composition were determined during fermentation. The total titratable acidity of the fermenting substrates varied between 0.07% for N. crassa fermented substrate to 0.26% for R. oryzae fermented substrate. While there was a general decrease in value for the pH of the fermenting substrates from 6.6 to 5.9. Proximate analysis revealed that the protein content of substrates increased from 26.21 g 100 g-1 in the control to 33.51 g 100 g-1 with N. crassa as the inoculum, while the fat content decreased from 11.95 g 100 g-1 in the control to 1.95 g 100 g-1 in the substrate fermented with A. niger, respectively. The mineral composition showed an increase in magnesium, sodium, potassium and iron compared with the control (30.07, 18.51, 23.51 and 0.00 μg g-1, respectively). The antinutrient composition revealed that both the canavanine and phytate content of the fermented substrates decreased significantly from 0.79 mg g-1 (control) to 0.53 mg g-1 (A. niger fermented substrate) and 5865.60 mg 100 mg-1 (control) to 1352.60 mg 100 g-1 (A. niger fermented substrate), respectively. Therefore, the use of single starter culture mould fermentation can be used to improve the nutritional quality of Canavalia ensiformis L.
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